Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Prevention of Orotic Acid-induced Fatty Liver in Rats by Capsaicin

Cha, Jae-Young;Jun, Bang-Sil;Cho, Young-Su

  • Published : 20041000
⟨ Previous Next ⟩

Abstract

Effects of capsaicin on triglyceride concentrations in liver and serum, and phosphatidate phosphohydrolase (PAP) activity involved in triglyceride synthesis in rats fed diets with or without 1% orotic acid were studied. Liver triglyceride concentration was markedly increased by orotic acid-feeding, whereas this raise was significantly reduced by simultaneous feeding of capsaicin and orotic acid. Orotic acid-feeding significantly increased activity of PAP, rate-limiting enzyme for triglyceride synthesis, while addition of 0.04% capsaicin to orotic acid-diet decreased the activity in liver. Serum glutamate-oxaloacetate transaminase (GOT) and glutamate-pyruvate transaminase (GPT) activities were not significantly different among experimental groups. Hepatocytes in orotic acid-induced fatty liver contained numerous lipid droplets, which were prevented by addition of capsaicin. Capsaicin reduces liver triglyceride accumulation and enhances PAP activity induced by orotic acid administration.

Keywords

References

  1. Bioscience Biotechnology and Biochemistry v.62 no.3 Association between hepatic triacylglycerol accumulation induced by administering orotic acid and enhanced phosphatidate phosphohydrolase activity in rats Cha, J.Y.;Mameda, Y.;Oogami, K.;Yamamoto, K.;Yanagita, T. https://doi.org/10.1271/bbb.62.508
  2. J. Pham. Exp. Ther. v.291 Down-regulation of rat hepatic microsomal cytochromes P-450 in microvesicular steatosis induced by orotic acid Su, G.M.;Sefton, R.M.;Murray, M.
  3. J. Lipid Res v.26 Early effects of dietary orotic acid upon liver lipid synthesis and bile cholesterol secretion in rats Tokmakjian, S.D.;Haines, D.S.M.
  4. Biochem. J. v.325 Decreased secretion of very-low-density lipoprotein triglyceride and apolipoprotein B is associated with decreased intercellular triglyceride lipolysis in hepatocytes derived from rats fed orotic acid or n-3 fatty acids Hebbachi, A.M.;Seelaender, M.C.L.;Baker, P.W.;Gibbons, G.F. https://doi.org/10.1042/bj3250711
  5. J. BioI. Chem. v.268 Transit and sorting of apolipoprotein B within the endoplasmic reticulum and Golgi compartments of isolated hepatocyte from normal and orotic acid-fed rats Catwright, I.J.;Hebachi, A.M.;Higgins, J.A.
  6. Biochirn. Biophys. Acta v.711 Reduction of beta-oxidation capacity of rat liver mitochondria by feeding orotic acid Miyazawa, S.;Furuta, S.;Hashimoto, T. https://doi.org/10.1016/0005-2760(82)90064-9
  7. Proc. Jpn. Com. Biochem. Lipids v.38 Triglyceride metabolism of fatty liver and the prevention by dietary n-3 fatty acid Yanagita, T.;Oogami, K.;Yamamoto, T.;Cha, J.Y.;Nunez, J.
  8. J. Jpn. Soc. Nutr. Food Sci. v.49 Effect of fish oil on liver lipid accumulation and decrease of serum lipid in rats dosed with orotic acid Hosoyamada, Y.;Kuroda, K.;Kobatake, Y. https://doi.org/10.4327/jsnfs.49.95
  9. J. Nutr. v.111 Effect of some dietary additions to either an arginine-devoid diet or a diet supplemented with orotic acid re-fed after starvation on liver lipid content during essential fatty acid deficiency in rats Aoyama, Y.;Yohida, A.;Ashida, K. https://doi.org/10.1093/jn/111.5.895
  10. Bioscience Biotechnology and Biochemistry v.56 no.1 Relationship between colonic products from com bran hemicellulose and the lipid metabolism in rats fed on diets supplemented with orotic acid Miyasaka, H.;Sanada, H.;Ayano, Y. https://doi.org/10.1271/bbb.56.157
  11. Plant Foods for Human Nutrition v.56 no.4 Effect of hesperetin, a citrus flavonoid, on the liver triacylglycerol content and phosphatidate phosphohydrolase activity in orotic acid-fed rats Cha, J.Y.;Cho, Y.S.;Kim, I.;Anno, T.;Rahman, S.M.;Yanagita, T. https://doi.org/10.1023/A:1011884200848
  12. Food Sci. Biotechnol. v.3 The changes in capsaicin, dihydrocapsaicin and capsanthin in Kochujangs with different mashing methods Chun, M.S.;Lee, T.S.;Noh, B.S.
  13. Carcinogenesis v.16 no.10 Chemoprotective effects of capsaicin and diallyl sulfide against mutagenesis or tumorigensis by vinyl carbomate and Nnitrosodimethylamine Surh, Y.J.;Lee, R.C.;Park, K.K.;Mayne, S.T.;Liem, A.;Miller, J.A. https://doi.org/10.1093/carcin/16.10.2467
  14. J. Food Sci. Nutr. v.7 Inhibitory effect of capsaicin against carcinogen-induced oxidative damage in rats Yu, R.;Choi, M.A.;Kawada, T.;Kim, B.S.;Han, I.S.;Yoo, H. https://doi.org/10.3746/jfn.2002.7.1.067
  15. British Journal Of Nutrition v.90 no.3 Effect of capsaicin on substrate oxidation and weight maintenance after modest body-weight loss in human subjects Lejeune, M.P.G.;Kovacs, E.M.R.;Westerterp-Pantenga, M.S. https://doi.org/10.1079/BJN2003938
  16. J. Jpn. Soc. Nutr. Food Sci. v.53 The effects of capsaicin on decrease of weight and consumption of energy in obesity rats by high-fat diet Yagi, N.;Noguchi, T.;Okada, A. https://doi.org/10.4327/jsnfs.53.227
  17. Bioscience Biotechnology and Biochemistry v.63 no.12 Antioxidative effects of turmeric, rosemary and capsicum extracts on membrane phospholipid peroxidation and liver lipid metabolism in mice Asai, A.;Nakagawa, K.;Miyanawa, T. https://doi.org/10.1271/bbb.63.2118
  18. Indian J. Med. Res. v.90 Effect of capsaicin on triglyceride accumulation and secretion in ethanol fed rats Sambaiah, K.;Satyanarayana, M.N.
  19. Journal of Ethnopharmacology v.75 no.2 Biphasic membrane effects of capsaicin, an active component in Capsicum species Tsuchiya, H. https://doi.org/10.1016/S0378-8741(01)00200-8
  20. J. Jpn. Soc. Nutr. Food Sci. v.56 Favorable effects of vanillylnonanamide-b-D-glucoside on serum lipids in hyperlipidemic rats Tani, Y.;Fujioka, T.;Hamada, H.;Kunimatsu, M.;Furuichi, Y. https://doi.org/10.4327/jsnfs.56.181
  21. J. BioI. Chem. v.226 A simple method for the isolation and purification of total lipids from animal tissues Folch, J.;Lees, M.;Sloane-Starley, G.H.
  22. J. BioI. Chem. v.193 Protein measurement with the folin phenol reagent Lowry, O.H.;Rosenbrough, N.J.;Farr, A.L.;Ranll, R.J.
  23. Biochim. Biophys. Acta v.796 The role of Mg2+-dependent phosphatidate phosphohydrolase in pulmonary glycerolipid biosynthesis Walton, P.A.;Possmayer, F.
  24. Biometrische Zeitschrift v.1 no.1 Multiple range and multiple F test Duncan, D.B. https://doi.org/10.1002/bimj.19590010102
  25. Bioscience Biotechnology and Biochemistry v.66 no.6 Effects of fatty liver induced by niacin-free diet with orotic acid on the metabolism of tryptophan to niacin in rats Fukudawatari, T.;Morikawa, Y.;Sugimoto, E.;Shidata, K. https://doi.org/10.1271/bbb.66.1196
  26. Lab. Invest. v.30 Production and prevention of fatty liver in rats fed clofibrate and orotic acid diets containing sucrose Novikoff, P.M.;Roheim, P.S.;Novikoff, A.B.;Edelstein, D.B.
  27. Proc. Soc. Exp. BioI. Med. v.90 Fatty liver induced by orotic acid feeding Standerfer, S.D.;Handler, P. https://doi.org/10.3181/00379727-90-22005
  28. J. BioI. Chem. v.236 Fatty liver induced by orotic acid. I. Accumulation and metabolism of lipids Creasey, W.A.;Hankin, L.;Handschumacher, R.E.
  29. J. Lipid Res. v.36 Assembly of very low density lipoprotein in rat liver: Astudy of nascent particles recovered from rough endoplasmic reticulum Swift, L.L.
  30. J. Nutr. v.116 Effects of capsaicin on lipid metaboilsm in rats fed high fat diet Kawada, T.;Hagihara, K.;Iwai, K. https://doi.org/10.1093/jn/116.7.1272
  31. Hepatology v.18 no.4 The activity of the metabolic form of hepatic phosphatidate phosphohydrolase correlates with the severity of alcoholic fatty liver in human beings Day, C.P.;James, O.F.;Brown, A.S.;Bennett, M.K.;Fleming, I.N.;Yeaman, S.J. https://doi.org/10.1002/hep.1840180413
  32. Lipids v.31 no.8 Dietary sunflower oil reduces plasma and liver triacylglycerols in fasting rats and is associated with decreased liver microsomal phosphatidate phosphohydrolase activity Fremont, L.;Gozzelino, M.T. https://doi.org/10.1007/BF02522983
  33. The American Journal of Chinese Medicine v.31 no.5 Protective effect of Nigella sativa seeds against carbon tetrachloride-induced liver damage Al-Ghamdi, M.S. https://doi.org/10.1142/S0192415X03001399
  34. Res. Commun. Mol. Pathol. Pharmacal. v.102 Liver-protective activities of aucubin derived from traditional oriental medicine Chang, I.M.
  35. Journal of Veterinary Medical Science v.60 no.4 Prevention of orotic acid-induced fatty liver in male rats by dihydroepiandrosterone and/or phenobarbital Goto, H.;Yamashita, S.;Makita, T. https://doi.org/10.1292/jvms.60.513
  36. Bioscience Biotechnology and Biochemistry v.64 no.9 Suppression of Dgalactosamine-induced liver injury by mushroom in rats Lee, E.W.;He, P.;Kawagishi, H.;Sugiyama, K. https://doi.org/10.1271/bbb.64.2001
  37. Tokai J. Exp. Clin. Med. v.11 Ultrastructural and enzyme histochemical studies in orotic acid-induced fatty liver Murakoshi, M.;Misawa, M.;Watanabe, K.
  38. Archiv der Pharmazie v.323 no.9 Hypolipidemic activity in rodents of phenobarbital and related derivatives Hall, I.H.;Patrick, M.A.;Maguire, J.H. https://doi.org/10.1002/ardp.19903230905