Isolation of Bacillus subtilis Producing the Cheongkukjang with Reduced Off-flavor by Suppressing the Growth of Bacteria Causing Off-flavor

Rhee, Joo-Hyung;Park, Ki-Hwan;Yoon, Kwang-Ro;Yim, Choong-Bin;Lee, In-Hyung

  • Published : 20041200

Abstract

Off-flavor compounds and microbial flora of newly developed Cheongkukjang with reduced off-flavor were compared to those of commercial Cheongkukjangs. Contents of unique and off-flavor compounds were 8.2 to 20.4 times lower in newly developed Cheongkukjang than other commercial products. Planococcus sp. and Marinococcus sp. at $7{\times}10^{7}$ and $1{\times}10^{7}$ cfu/g, respectively, were detected in commercial Cheongkukjangs, but not in newly developed Cheongkukjang, suggesting that these microbial flora affect Cheongkukjang flavor. Slime of newly developed Cheongkukjang suppressed growths of Planococcus sp. and Marinococcus sp. found in straw used for newly developed Cheongkukjang fermentation, whereas those of commercial Cheongkukjangs did not. Results suggest B. subtilis existing in newly developed Cheongkukjang suppressed growths of off-flavor-causing Planococcus sp. and Marinococcus sp..

Keywords

References

  1. Korean J. Appl. Microbiol. Biotechnol. v.26 Medium optimization for fibrinolytic enzyme production by Bacillus subtilis KCK-7 isolated from Korean traditional Cheongkukjang Lee, S.K.;Heo, S.;Bae, D.H.;Choi, K.H.
  2. Korean J. Appl. Microbiol. Biotechnol. v.26 Purification and charaterization of fibrinolytic enzyme excreted by Bacillus subtilis K-54 isolated from Chung Guk Jang Yoo, C.K.;Seo, W.S.;Lee, C.S.;Kang, S.M.
  3. J. Korean Soc. Food Sci. Nutr. v.27 Production and characterization of fibrinolytic enzyme: Optimal condition for production of the enzyme from Bacillus sp. KP-6408 isolated from Chungkookjang Kil, J.O.;Kim, G.N.;Park, I.
  4. Appl. Environ. Microbiol. v.62 Purification and characterization of fibrinolytic enzyme produced from Bacillus sp. strain CK-11-4 screened from Chungkook-jang Kim, W.;Choi, K.;Kim, Y.;Park, H.;Choi, J.;Lee, Y.;Oh, H.;Kwon, I.;Lee, S.
  5. Microorganism Ind. v.23 Present status of industries and research activities of Korean fermented soybean products Yoo, J.Y.
  6. Korean J. Food Sci. Technol. v.35 Characteristics of flavor and functionality of Bacillus subtilis K-20 Chunggukjang Kim, Y.S.;Jung, H.J.;Park, Y.S.;Yu, T.S.
  7. Korean J. Food Sci. Technol. v.21 Changes in flavor of Cheongkukjang during fermentation Choi, S.H.;Ji, Y.A.
  8. J. Korean Soc. Food Sci. Nutr. v.27 Characteristics of Chunggugjang produced by Bacillus subtilis DC-2 Choi, U.K.;Ji, W.D.;Chung, Y.G.
  9. J. Korean Soc. Agric. Chem. Biotechnol. v.42 Volatile compounds of Chonggugjang prepared by different fermentation methods and soybean cultivars Choe, J.S.;Yoo, S.M.;Kim, H.R.;Kim, J.S.;Chang, C.M.
  10. J. Korean Soc. Hyg. Sci. v.8 Changes of flavor during Cheongkukjang fermentation by Bacillus sp. CS-17 Choi, U.K.;Lee, S.I.;Son, D.H.;Ji, W.D.
  11. Food Sci. Technol. v.13 Characteristic flavor compounds of traditional Korean Chongkukjang Lee, E.J.;Kim, J.K.
  12. International Journal of Food Microbiology v.29 no.2 Control of ammonia formation during Bacillus subtilis fermentation of legumes Allagheny, N.;Obanu, Z.A.;Campbell-Platt, G.;Owens, J.D. https://doi.org/10.1016/0168-1605(95)00069-0
  13. J. Korean Soc. Food Nutr. v.24 Flavor component, fatty acid and organic acid of natto with spice added Kim, B.N.;Park, C.H.;Ham, S.S.;Lee, S.Y.
  14. Studies on chemical composition of commercial Cheongkukjang and flavor compounds of Cheongkukjang by Mugwort (Artmisia asiatica) or Red pepper seed oil Joo, H.K.
  15. Korean J. Food Sci. Technol. v.34 Flavor improvement of Cheongkukjang by addition of Yucca (Yucca shidigera) extract In, J.P.;Lee, S.K.;Ahn, B.K.;Chung, I.M.;Jang, C.H.
  16. The method for Cheongkukjang preparation and its use for the manifature of hamberger patti Suh, B.R.;Yim, C.B.
  17. J. Korean Soc. Food Sci. Nutr. v.31 Quality characteristics of the Cheongkukjang fermented by the mixed culture of Bacillus natto and B. licheniformis Youn, K.C.;Kim, D.H.;Kim, J.O.;Park, B.J.;Yook, H.S.;Cho, J.M.;Byun, M.W. https://doi.org/10.3746/jkfn.2002.31.2.204
  18. Agric. Chem. Biotechnol. v.37 Change of protein and amino acid composition during Cheongkukjang fermentation using Bacillus licheniformis CN-115 Seok, Y.R.;Kim, Y.H.;Kim, S.;Woo, H.S.;Kim, T.W.;Lee, S.H.;Choi, C.
  19. Morphology;Manual of Methods for General Bacteriology Murray, R.G.E.;Gerhardt, P.(ed.)
  20. Practical Atlas for Bacterial Identification Cullimore, D.R.
  21. Bergey's Manual of Determinative Bacteriology Holt, J.G.;Krieg, N.R.;Sneath, P.H.A.;Staley, J.T.;Williams, S.T.
  22. J. Food Hyg. Saf. v.16 Antimicrobial activities of viscous substance from Chongkukjang fermented with different Bacillus spp Youn, H.K.;Choi, H.S.;Hur, S.H.;Hong, J.H.