Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effect of Cellulose Degrading Bacteria Isolated from Wild and Domestic Ruminants on In vitro Dry Matter Digestibility of Feed and Enzyme Production

  • Sahu, N.P. (Central Institute for Research on Buffaloes) ;
  • Kamra, D.N. (Microbiology Section, Centre of Advanced Studies in Animal Nutrition, Indian Veterinary Research Institute) ;
  • Paul, S.S. (Central Institute for Research on Buffaloes)
  • Received : 2002.11.14
  • Accepted : 2003.08.21
  • Published : 2004.02.01
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Abstract

Cellulolytic bacterial strains have been isolated from the faeces of wild (blackbuck, Antilope cervicapra; nilgai, Baselophus tragocamelus chinkara, Gazella gazella spotted deer, Axis axis and hog deer, Cervus porcinus) and rumen liquor of domestic (sheep, Ovis aries) ruminants. Five best cellulose degrading bacterial isolates (Ruminococcus sp.) were used as microbial feed additive along with buffalo rumen liquor as inoculum to study their effect on digestibility of feed and enzyme production in in vitro conditions. The bacterial isolate from chinkara (CHI-2) showed the highest per cent apparent dry matter (DM) digestibility ($35.40{\pm}0.60$), true dry matter digestibility ($40.80{\pm}0.69$) and NDF ($26.38{\pm}0.83$) digestibility (p<0.05) compared to control ($32.73{\pm}0.56$, $36.64{\pm}0.71$ and $21.16{\pm}0.89$, respectively) and other isolates at 24 h of incubation with lignocellulosic feeds (wheat straw and wheat bran, 80:20). The same isolate also exhibited the highest activities of fibre degrading enzymes like carboxymethylcellulase, xylanase, ${\beta}$-glucosidase and acetyl esterase. The bacterial isolate from chinkara (Gazella gazella) appears to have a potential to be used as feed additive in the diet of ruminants for improving utilization of nutrients from lignocellulosic feeds.

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References

  1. Agarwal, N., I. Agarwal, D. N. Kamra and L. C. Chaudhary. 2000. Diurnal variation in the activities of hydrolytic enzymes in diffrent fractions of rumen contents of Murrah buffaloes. J. Appl. Anim. Res. 18:73-80.
  2. Agarwal, N., N. Kewalramani, D. N. Kamra, D. K. Agarwal and K. Nath. 1991. Hydrolytic enzymes of buffalo rumen comparison of cell free rumen fluid, bacterial and protozoal fractions. Buffalo J. 7:203-207.
  3. Baumgardt, B. R., M. W. Taylor and T. L. Cason. 1962. Evaluation of forages in the laboratory. ii Simplified artificial rumen procedure for obtaining repeatable estimates of forage nutritive value. J. Dairy Sci. 45:62.
  4. Bryant, M. P. and L. A. Burkey. 1953. Cultural methods and some characteristics of some of the more numerous groups of bacteria in the bovine rumen. J. Dairy Sci. 36:205-217.
  5. Cheng, K. J., C. W. Forseberg, H. Minato and J. W. Costerton. 1991. Microbial physiology and ecology of feed degradation within the rumen. In: (Ed. T. Tsuda, Y. Sasaki and R. Kawashima) Physiological aspects of digestion and metabolism in ruminants. Academic Press, Toronto, Otario, Canada.
  6. Francois Domingue, B. M. D. W. Dellow, P. R. Wilson and T. N. Barry. 1991. Comparative digestion in deer, goats and sheep. Newzealand J. Agric. Res. 34:45-53. https://doi.org/10.1080/00288233.1991.10417792
  7. Goering, H. K. and P. J. Van Soest. 1989. Forage fibre analyses (apparatus, reagents, procedures and some applications). Agriculture Hand book no. 379-ARS-USDA, Washington, DC.
  8. Ha, J. K., S. S. Lee, S. W. Kim, In K. Han, K. Ushida and K. Cheng. 2001. Degradation of rice straw by rumen fungi and cellulolytic bacteria through mono, co or sequential culture. Asian-Aust. J. Anim. Sci. 14:797-802.
  9. Johnson, K. G., J. D. Fontana and C. R. Mackenzie. 1988. In: (Ed. W. A. Wood and S. T. Kellogg) Methods of Enzymology. Academic Press, San diego, CA, 160:551-560.
  10. Kamra, D. N., S. Saha, N. Bhatt, L. C. Chaudhary and N. Agarwal. 2003. Effect of diet on enzyme profile, biochemical changes and in sacco degradability of feeds in the rumen of buffalo. Asian-Aust. J. Anim. Sci. 16:374-379.
  11. Latupeinissa, C. and G. Dryden. 1998. Comparative in vitro digestibility of feedstuffs by deer and sheep. Proc. Nutr. Soc. Aust. 22:53.
  12. Miller, G. L. 1959. Use of dinitrosalisilic acid reagent for determination of reducing sugars. Anal. Chem. 31:426-428. https://doi.org/10.1021/ac60147a030
  13. Ru, Y. J., P. C. Glatz, Z. H. Miao, K. Swanson, S. Falkenberg and S. Wyatt. 2002. Comparison of digestibility of grain and forage by sheep, red and fallow deer. Asian-Aust. J. Anim. Sci. 15:800-805.
  14. Sahu, N. P. 2002. Studies on cellulose degrading bacteria isolated from small ruminants and their effect on nutrient utilization in buffaloes. Ph.D Thesis. Indian Veterinary Research Institute, Deemed to be University, Izatnagar, India.
  15. Sahu, N. P. and D. N. Kamra. 2002. Microbial ecosystem of the gastro-intestinal tract of wild herbivorous animals. J. Appl. Anim. Res. 21:207-230.
  16. Shewale, J. G. and J. C. Sadana. 1978. Cellulase and $\beta$-glucosidase by a basidomycete species. Can. J. Microbiol. 24:1204-1216.
  17. Snedecor, G. W. and W. G. Cochran. 1989. Statistical Methods, 8th edn, Iowa state University, Iowa, USA.
  18. Tarakanov, B. V. and D. Y. Lavlinskii. 1998. Cellulolytic bacteria of the genus Ruminococcus from bovine rumen. Microbiology-New York 67:425-428.
  19. Teather, R. and P. J. Wood. 1982. Use of congo-red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl. Environ. Microbiol. 43:777-780.
  20. Tilley, J. M. A. and R. A. Terry. 1963. Two stage technique for in vitro digestion of forage crops. J. Br. Grassland Soc. 18:104-109.
  21. Van Soest, P. J. and J. B. Robertson. 1985. A laboratory manual for Animal Science 612, Cornell University, USA.

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