Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effect of Follicular Fluid Proteins and Gonadotropins on Progesterone Secretion by Buffalo Granulosa Cells In vitro

  • Vinze, Mukesh (Division of Animal Biochemistry, National Dairy Research Institute) ;
  • Sharma, M.K. (Division of Animal Biochemistry, National Dairy Research Institute) ;
  • Singh, Dheer (Division of Animal Biochemistry, National Dairy Research Institute)
  • Received : 2003.10.30
  • Accepted : 2004.06.16
  • Published : 2004.11.01
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Abstract

In the mammalian ovary the follicular fluid contains proteins and peptides which play an important role in growth, development and maturation of oocytes. The gonadotropins and some other factors work synergistically and regulate ovarian functions. In the present study the effect of follicular fluid proteins (FFP) and gonadotropins on progesterone secretion by granulosa cells (GC) from buffalo ovary, was investigated during culture. The follicular fluid was collected from small (<5 mm), and medium (5-8 mm) follicles obtained from buffalo ovaries. The follicular fluid from medium follicles was fractionated with ammonium sulphate at 80% saturation. The precipitated protein fraction was further resolved in to minor (peaks I, III) and major (peak II) proteins using gel filtration (Sephadex G-200). The FFP from small follicles and major FFP (peak II) at a dose of 200 $\mu$g/well, significantly stimulated progesterone secretion by pooled GC (3${\times}10^{5}$ cells/2 ml medium/well). The minor FFP did not show any stimulatory effect. There was a significant increase in progesterone secretion by pooled GC in presence of FFP and LH (10 ng/well), however, FSH (20 ng/well) with FFP exhibited an inhibitory effect. The major FFP and gonadotropins were also studied for their effect on progesterone production by GC isolated from medium and large size follicles. The GC from medium follicles were more responsive to FSH and FFP whereas GC from large follicles exhibited enhanced progesterone secretion with LH and FFP. These results indicated that FFP have their own stimulatory effect and also act synergistically with gonadotropins. The significantly different response shown by GC, for steroid hormone secretion, is based on their stage of growth and differentiation. The purification and characterization of such steroidogenic proteins may help in elucidating their role in growth and differentiation of granulosa cells.

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References

  1. Alpizer, E. and L. J. Spicer. 1993. Effect of interleukin on proliferation and follicle stimulating hormone-induced estradiol production by bovine granulosa cells, in vitro: Dependence on size of follicles. Biol. Reprod. 49:38-43. https://doi.org/10.1095/biolreprod49.1.38
  2. Andrews, P. 1965. Estimation of molecular weight of proteins by Sephadex gel filteration. Biochem. J. 91:222-223.
  3. Asem, E. K., F. Lintner, H. V. Biellier, W. H. Burke and F. Hertelendy. 1983. Comparision of turkey LH and ovine LHinduced progesterone production in granulosa cells of the turkey and of the domestic fowl. Gen. Comp. Endocrinol. 52: 445-451.
  4. Bendel, J. J., D. X. Lobb, A. Chuma, M. Gyster and J. H. Dorrington. 1988. Bovine theca cells secrete factor(s) that promote granulosa cells proliferation. Biol. Reprod. 38:780-86. https://doi.org/10.1095/biolreprod38.4.780
  5. Cabrera, V. O., N. V. Andino and M. D. Acosta. 1986. Protein electrophoretic patterns of bovine and porcine ovarian follicular fluid. J. Endocrinol. Invest. 8:489-493.
  6. DiZerega, G. S., S. A. Tonetta and G. Westhof. 1987. A postulated role for naturally occurring aromatase inhibitors in follicle secretion. J. Steroid Chem. 27:375-383.
  7. Hillensjo, T., S. Chari, L. Nilsson, L. Hamberger, E. Daume and G. Sturm. 1983. Inhibition of progesterone secretion in cultured human granulosa cells by a low molecular weight fraction of human follicular fluid. J. Clin. Endocrinol. Metabol. 56:835-838.
  8. Huang, C. J., Y. Li, M. H. Stromer and L. L. Anderson. 1992. Synergistic effect of insulin-like growth factor-I and gonadotropins on relaxin and progesterone secretion by aging corpora lutea of pigs. J. Reprod. Fert. 96:415-425.
  9. Huang, Wei-Tung, Lu She-Ghi, Tang Pin-Chi, Wu Shinn-Chih, Cheng San-Pao and Ju Jyh-Charng. 2002. Biochemical compositions of follicular fluid and the effects of Culture conditions on in vitro development of pig oocytes. Asian-Aust. J. Anim. Sci. 15:1403-1411.
  10. Kamboj, M. and B. S. Prakash. 1993. Relationship of progesterone in plasma and whole milk of buffaloes during cyclicity and early pregnancy. Trop. Anim. Health Prod. 25:185-192.
  11. Khan, S. A., P. Halin, J. Bartlett, Ch. De Geyter and E. Nieschlag. 1988. Characterization of a factor from human ovarian follicular fluid which stimulates Leydig cell testosterone production. Acta Endocrinol. (Copen) 118:283-290.
  12. Khan, S. A., C. Keck, T. Gudermann and E. Nieschlag. 1990. Isolation of protein from human follicular fluid which exerts major stimulatory effects on in vitro steroid production of testicular , ovarian and adrenal cells. Endocrinology 126:3043-52.
  13. Kigawa, T., T. Ogawa, R. Matsuoka and S. Koike. 1986. Inhibitory effect of the procine follicular fluid on estradiol and progesterone secretion by cultured rat granulosa cells. Endocrinol. JPN 33:221-232.
  14. Kulkarni, R. A. 1988. Follicular fluid proteins of Indian buffalo. Proceedings of II World Buffalo Congress held in India, during Dec. 12-16.
  15. Kumar, S. and H. C. Pant. 1990. Purification of inhibitor(s) from buffalo follicular fluid to compensatory ovarian hypertrophy in mice. Indian J. Anim. Reprod. 11:135-140.
  16. Laemmli, U. K. 1970. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227:680-685. https://doi.org/10.1038/227680a0
  17. May, J. V. and D. W. Schomberg. 1981. Granulosa cells differentiation in vitro. Effect of insulin on growth and functional integrity. Biol. Reprod. 25:421-431.
  18. McArdle, C. A., C. Kohl, K. Reiger, I. Groner and U. Wehrenberg. 1991. Effect of gonadotropins, insulin and insulin-like growth factor-I on ovarian oxytocin and Progesterone production. Mol. Cell. Endocrinol. 78:211-220.
  19. McGaughey, R. W. and J. C. Danniel. 1972. Protein patterns of ovarian follicular fluid from pigs. Biol. Reprod. 7:128-135.
  20. Mishra, O. P., J. N. Pandey and P. G. Gawande. 2003. Study on biochemical constituents of caprine ovarian follicular fluid after superovulation. Asian-Aust. J. Anim. Sci. 16:1711-1715.
  21. Monniaux, D. and C. Pisselet. 1992. Control of proliferation and differentiation of ovine granulose cells by insulin-like growth factor-I and follicle stimulating hormone in vitro. Biol. Reprod. 46:109-119.
  22. Nandedkar, T. D., A. L. Kadam, S. B. Moodbidri. 1988a. Control of follicular maturation in mouse by a non-steroidal regulator from sheep follicular fluid. Internatl. J. Fertil. 33:52-59.
  23. Shalgi, R., P. F. Kraicer, A. Rimon, M. Pinto and N. Soferman. 1973. Proteins of human Follicular fluid: The blood follicles barrier. Fert. Steril. 24:429-434. https://doi.org/10.1016/S0015-0282(16)39730-8
  24. Shiraishi, S. 1986. Studies on inhibin in human follicular fluid. Acta Obstet. Gynaecol. JPN 38:741-749.
  25. Singh, D., M. K. Sharma and R. S. Pandey. 1999. Biochemical and hormonal characterization of follicles from follicular and luteal phase ovaries of goat and sheep. Indian J. Expt. Biol. 37:434-438.
  26. Sluss, P. M., A. L. Schneyder, M. A. Franke, L. E. Reichert Jr.. 1987. Porcine follicular fluid contains both follicle stimulating hormone agonist and antagonist activities. Endocrinology 120:1477-1485.
  27. Snedecor, G. W. and W. G. Cochran. 1967. Statistical methods (6th Ed.) Oxford and IBH Publ. Co., New Delhi.
  28. Stein, A. L., G. S. DiZerega and S. A. Tonetta. 1990. Steroidogenesis in procine follicles cells cultured in serum-free media. Gynaecol. Obstet. Invest. 29:285-288.
  29. Tsafriri, A. 1988. Local non-steroidal regulators of ovarian function . In: 'Physiology of Reproduction' Vol.1, (Ed. E. Khobil and J. Neill), Raven Press, N.Y. pp. 527-565.
  30. Wang, L. J., M. Brannstrom, S. A. Robertson and R. J. Norman. 1992. TNF-alpha in the human ovary: presence in follicular fluid and effect on cell proliferation and prostaglandin production. Fertil. Steril. 58:931-940.
  31. Wickings, E. J. 1986. Gonadotropic control of steroidogenesis in human granulosa lutein cells. J. Reprod. Fertil. 76:677-684. https://doi.org/10.1530/jrf.0.0760677

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