Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
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Elimination of Lily Symptomless Virus through Shoot Apex Culture in Lilium Oriental Hybrids

오리엔탈나리의 경정배양에 의한 lily symptomless virus 제거

woo, Jin-Ha;Nam, Hyo-Hoon;Choi, Kyeong-Bae;Park, In-Sook;Kim, Kiu-Weon
우진하;남효훈;최경배;박인숙;김규원

  • Published : 20040000
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Abstract

This study was conducted to eliminate the lily symptomless virus (LSV) from LSV-infected Lilium Oriental Hybrids ‘Casa Blanca’ and ‘Marco Polo’ by shoot apex culture. Filamentous virus of 650nm and spherical virus of 30nm were detected in leaves of virus-infected plants of ‘Casa Blanca’ with DN method. The particles of filamentous and spherical viruses were sporadically distributed in cytoplasm of leaf cell. The filamentous virus was estimated by LSV with ISEM. Virus-infected plants of ‘Casa Blanca’ were presumed to be in the state of complex infection of LSV and other viruses. More number of leaf primordia attached to shoot apical meristem, larger size of shoot apex explant resulted in the promotion of the survival rate and enlargement of bulblets. The result of virus detection for plantlets taken from in vitro culture and after-generation in vitro by DTBIA method shows that LSV was eliminated completely from ‘Casa Blanca’ solely with cultured shoot apical meristem and ‘Marco Polo’ with one leaf primodium attached to shoot apcial meristem. From above result, LSV is considered not to exist in shoot apical meristem of Lilium Oriental Hybrids ‘Casa Blanca’ and ‘Marco Polo’.

본 연구는 경정배양에 의해 오리엔탈나리 ‘카사블랑카’ 및 ‘마르코폴로’의 LSV 감염주로부터 LSV를 제거하고자 하였다. ‘카사블랑카’의 바이러스 감염주의 잎에서는 650nm의 사상형 바이러스와 30nm 정도의 구형 바이러스 입자가 DN법으로 관찰되었다. 사상형과 구형의 바이러스 입자는 잎 세포의 세포질에 산재하였다. 사상형 바이러스는 ISEM법에 의해 LSV로 추정되었다. ‘카사블랑카’의 바이러스 감염주는 LSV와 다른 바이러스에 복합감염되어 있는 것으로 추정되었다. 기내에서의 생존율과 소인경의 비대는 경정분열조직에 엽원기 수가 많을수록 즉 경정외식체의 크기가 클수록 증가하였다. 경정배양에 의한 LSV의 제거율은 기내 수확당시와 기내다음세대에서 맹아된 잎을 채취하여 DTBIA법으로 검정한 결과 ‘카사블랑카’는 경정분열조직만 배양했을 때, ‘마르코폴로’는 경정분열조직에 엽원기가 1매 이하 부착되었을 때 완전히 제거되었다. 이로 봐서 오리엔탈나리 ‘카사블랑카’의 경정분열조직에는 LSV가 존재하지 않는 것으로 판단되었다.

Keywords

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