Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Urease-positive Vibrio parahaemolyticus Isolated from Kamak Bay in Yeosu in 2002 and 2003

Park, Mi-Yeon

  • Published : 20040800
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Abstract

Among 92 Vibrio parahaemolyticus strains isolated, only 5 were urease-positive, and all 92 strains showed no hemolysin activity. V. parahaemolyticus YKB4 and YKB 14 were isolated from seawater, YFB20 from black rockfish (Sebases schlegeli), and YFO21 and YFO22 from olive flounder (Paralichthys olivaceus). The five urease-positive strains showed no hemolysin and protease activities, but showed biochemical characteristics identical to reference strain, V. parahaemolyticus KCTC2471, except in urease production. They showed urease activities at mid-stationary phase, and maximal activity at late-stationary phase. Mortalities by urease-positive V. parahaemolyticus YKB4, YKB14, YFO21, and YFO22 were significantly high, 60-80%, while YFB20 showed 20% in ICR mice. Protease-positive V. parahaemolyticus FM39 and FM50 showed 40 and 60% mortality, respectively. Hemolysin-positive V. parahaemolyticus showed no mortality, as with non-pathogenic V. parahaemolyticus KCTC2471. Mortality by urease-positive V. parahaemolyticus strains occurred within 12 hr of injection, and vibrio strains could be isolated from viscera of injected mice.

Keywords

References

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