Applied Chemistry for Engineering (공업화학)

The Korean Society of Industrial and Engineering Chemistry (한국공업화학회)
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Study on the Antibacterial Effects of Silver Nanoparticles Prepared by Sonication Method

초음파법으로 제조된 은 나노 입자의 항균특성 연구

Cho, Kyung-Hwan;Park, Soo-Gil
조경환;박수길

  • Published : 20041200
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Abstract

The antibiological effect of nanoparticles on staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) using silver nanoparticles (Ag-NPs), < 10 nm, prepared by the sonication of $AgClO_4$ was studied. To determine the antibacterial activity, we applied $100\;{\mu}L$ of 0.054% Ag-NPs solution to each culture medium containing test organism and evaluated the antibacterial activities by the cup diffusion method. Only Ag-NPs stabilized with poly(N-vinyl 2-pyrrolidone) [PVP] showed antibacterial activity and the minimum inhibitory concentration (MIC) to S. auresu and E. coli were 5 ppm. And the complete sterilizing concentrations for the test organisms were 50 and 100 ppm, respectively. Also we observed the morphological alteration and cell damages caused by Ag-NPs treatment using a scanning electron microscopy (SEM). Above results supports the evidence that Ag-NPs have strong antibacterial activity to S. aureus and E. coli.

본 연구에서는 염화은($AgClO_4$)을 초음파 처리하여 얻은 10 nm 이하의 은 나노입자의 항균력을 측정하기 위해 그램 양성균인 Staphylococcus aureus (S. aureus)와 그램 음성균인 Escherichia coli (E. coli)를 배양한 배지에 0.054%은 나노용액 $100\;{\mu}L$를 넣고 cup diffusion method로 실험을 행하였다. 그 결과 poly(N-vinyl 2-pyrrolidone) [PVP]로 안정화시킨 은 나노 입자에서만 항균활성을 보였으며 S. aureus와 E. coli에 대한 최소 억제농도(minimum inhibitory concentration, MIC)는 5 ppm이었으며 완전 살균농도는 50 ppm과 100 ppm이었다. 은 나노입자에 의해 사멸된 균의 형태상 변화와 세포 손상을 scanning electron microscopy (SEM)으로 확인하였다. 또한 은 나노 입자가 양성균(황색포도상 구균)과 음성균(대장균)에서의 특수한 향균 효과를 증명할 수 있었다.

Keywords

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