Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
권호 표지

The Transformation of a Barley Cultivar, “Saechalssalbori” using Particle Bombardment

유전자총에 의한 새찰쌀보리 미숙배 형질전환

Choi, Chang-Hyun;Yun, Choong-Hyo;Hwang, Cheol-Ho
최창현;윤충효;황철호

  • Published : 20040000
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Abstract

Although barley is the fourth most important cereal crop in the world next to wheat, maize, and rice, efficient transformation method has not been established using Korean domestic barley. In order to find several parameters involved in particle bombardment, Bobwhite, a wheat cultivar showing a high efficiency of tranformation was examined in parallel with to Saechalssalbori, a domestic barley. The different sets of transformation with varying amounts of DNA and gold particle were performed using immature embryos as target explants. The highest levels of a transient GUS expression were shown in combinations of 4 $\mu$g of DNA, 400 $\mu$g of gold particle in Bobwhite and 7 $\mu$g of DNA, 400 $\mu$g of gold particle in Saechalssalbori, respectively. Immature embryos were transformed under different bombardment conditions in two levels of pressures (1100 psi, 900 psi), three levels of micro-carrier flying distances (6 cm, 9 cm, 12 cm) and two size of the particle (1 $\mu$m, 0.6 $\mu$m). The result showed the most stable transient expression of GUS with 1 $\mu$m of particle at 1100 psi in both Bobwhite and Saechalssalbori. Using immature embryos of Bobwhite, the embryogenic callus induced two times more in DC medium under a dim light and a regeneration efficiency showed six times more than any other combination examined. In general a pre-conditioning of osmotic treatment for 4 hours of immature embryo and calli derived from mature embryos had increased the number of transgenic calli only in the DC medium under a dim light.

Keywords

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