Journal of Apiculture (한국양봉학회지)

The Apicultural Society of Korea (한국양봉학회)
권호 표지

Anti-inflammatory Effect of the venom from Asian honeybees (Apis cerana L.) on Inducible Nitric Oxide Synthase and Tumor Necrosis Factor-α in RAW 264.7 Cell Line

국내산 동양종 꿀벌의 벌독에 의한 RAW 264.7 세포주에서 Nitric Oxide와 Tumor Necrocis Factor-a에 미치는 항염증 작용에 관한 연구

Han, Sang-Mi;Yeo, Ju-Hong;Lee, Myeong-Ryeol;Lee, Man-Yeong;Park, Gwan-Gyu;Gwon, Hae-Yong;Lee, Gwang-Gil;U, Sun-Ok;Baek, Ha-Ju
한상미;여주홍;이명렬;이만영;박관규;권해용;이광길;우순옥;백하주

  • Published : 20041200
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Abstract

Several investigators have evaluated the effects of honeybee venom (Apis mellifer L.) to biological activity such as anti-inflammatory and antinociceptive effects. But, the biological activity of the venom from Asian honeybees (Apis cerana L.) is not evaluated yet. In this study, the production of proinflammatory cytokines, nitric oxide (NO) and tumor necrosis factor-α (TNF-α), was examined in a macrophage like cell line, RAW 264.7 cells, in the presence of the venom from Asian honeybees (A. cerana) water-soluble (BVW) extracts. RAW 264.7 cells activated with lipopolysaccharide (LPS) were treated with various doses of BVW extracts. Supernatants were analyzed for the production of NO and TNF-α using Griess reagent and enzyme-linked immunosorbent assay (ELISA), respectively. BVW extracts up to 1 ug/ml still required to inhibit NO and TNF-α induced by LPS. Thease results suggest the possibility that BVW may be effective therapeutic agents for inflammatory diseases.

Keywords

References

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