Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Optimized Production of Lacticin NK24, a Bacteriocin Produced by Lactococcus lactis NK24 Isolated from Jeot-gal

Lee, Na-Kyoung;Kim, Kee-Tae;Kim, Cheon-Jei;Paik, Hyun-Dong

  • Published : 20040200
⟨ Previous Next ⟩

Abstract

Lactococcus lactis NK24 isolated from Jeot-gal (Korean traditional fermented fish-based food) produces lacticin NK24. Effects of several parameters on the production of lacticin NK24 by L. lactis NK24 were studied. MRS medium was selected for enhanced production of the bacteriocin. Bacteriocin activity during pH 6-controlled production was higher than that of pH-uncontrolled one. The highest lacticin NK24 activity (1,638,400 AU/mL) in batch fermentation was obtained when fermentation paramenters such as pH 6.0, $32^{\circ}C$, and 1% inoculum (v/v) were used. Enhanced production of lactic in NK24 was investigated in fed-batch fermentations using different sets of carbon and/or nitrogen sources. Production of lacticin NK24 increased by feeding of 10 g/L of each glucose and peptone. Under optimized conditions, lacticin NK24 activity reached 6,553,600 AU/mL in fed-batch, fourfold higher than that in batch fermentation.

Keywords

References

  1. Biochimie v.70 no.3 Bacteriocins of lactic acid bacteria Klaenhammer, T.R. https://doi.org/10.1016/0300-9084(88)90206-4
  2. Food Sci. Biotechnol. v.12 Isolation of a bacteriocin-producing Lactobacillus plantarum strain from Kimchi Kim, H.T.;Park, J.Y.;Lee, G.G.;Kim, J.H.
  3. J. Gen. Microbiol. v.138 Influence of the carbon source on nisin production in Lactococcus lactis subsp. lactis batch fermentations De Vuyst, L.;Vandamme, E.J.
  4. Applied Microbiology and Biotechnology v.48 no.4 The effect of nisin concentration and nutrition depletion on nisin production of Lactococcus lactis Kim, S.W.;Hall, R.J.;Dunn, N.W. https://doi.org/10.1007/s002530051078
  5. Bioscience Biotechnology and Biochemistry v.62 no.5 production by Lactococcus lactis IO-1 using xylose as carbon source Chinachoti, N.;Matsusaki, H.;Sonomoto, K.;Ishizaki, A.;Nisin, Z. https://doi.org/10.1271/bbb.62.1022
  6. Journal of Industrial Microbiology and Biotechnology v.18 no.1 Growth and bacteriocin production by Enterococcus faecium DPCI146 in batch and continuous culture Parente, E.;Brienza, E.;Ricciardi, Addario G. https://doi.org/10.1038/sj.jim.2900368
  7. Applied Microbiology and Biotechnology v.45 no.5 Continuous production of pediocin by immobilized Pediococcus acidilactici PO2 in a packed-bed bioreactor Cho, H.Y.;Yousef, A.E.;Yang, S.T. https://doi.org/10.1007/s002530050734
  8. Biotechnology Letters v.23 no.8 Production of a novel bioflocculant by fed-batch culture of Citrobacter sp Jang, J.H.;Ike, M.;Kim, S.M.;Fujita, M. https://doi.org/10.1023/A:1010312607171
  9. Food Microbiology v.18 no.1 Partial characterization of lactic in NK24, a newly identified bacteriocin of Lactococcus lactis NK24 isolated from Jeot-gal Lee, N.K.;Paik, H.D. https://doi.org/10.1006/fmic.2000.0368
  10. Food Sci. Biotechnol. v.12 Partial characterization of a bacteriocin produced by Propionibacterium freudenreichii KCCM 31227 Paik, H.D.;Park, H.J.
  11. Journal of Applied Microbiology v.91 no.3 Factors affecting production of an antilisterial bacteriocin by Carnobacterium piscicola strain A9b in laboratory media and model fish systems Himelbloom, B.;Nilsson, L.;Gram, L. https://doi.org/10.1046/j.1365-2672.2001.01401.x
  12. Journal of Applied Microbiology v.88 no.4 Production of a nisin-like bacteriocin by Lactococcus lactis subsp. lactis A164 isolated from Kimchi Choi, H.J.;Cheigh, C.I.;Kim, S.B.;Pyun, Y.R. https://doi.org/10.1046/j.1365-2672.2000.00976.x
  13. Appl. Environ. Microbiol. v.45 Lantibiotic nisin Z fermentative production by Lactococcus lactis IO-1: relationship between production of the lantibiotic and lactate and cell growth Matsusaki, H.;Endo, N.;Sonomoto, K.;Ishizaki, A.
  14. J. Appl. Bacteriol. v.75 The influence of pH and growth rate on production of the bacteriocin, bavaricin MN, in batch and continuous fermentations Kaiser, A.L.;Montville, T.J.
  15. Journal of Applied Microbiology v.87 no.3 Nisin, temperature and pH effects on growth and viability of Pectinatus frinsingensis, a Gram-positive, strictly anaerobic beer-spoilage bacterium Chihib, N.E.;Monnerat, L.;Membre, J.M.;Tholosan, J.L. https://doi.org/10.1046/j.1365-2672.1999.00841.x
  16. Appl. Environ. Microbiol. v.65 Nisin production by a mixed-culture system consisting of Lactococcus lactis and Kluyveromyces marxianus Shimizu, H.;Mizuguchi, T.;Tanaka, E.;Shioya, S.
  17. Korean J. Appl. Microbiol. Biotechnol. v.20 Cultural conditions of Lactococcus sp. 112-1 for production of bacteriocin-like substance Yoo, J.Y.;Lee, I.S.;Chung, K.S.;Choi, S.Y.;Koo, Y.J.;Kwon, D.J.
  18. Journal of Applied Microbiology v.86 no.3 Production of a nisin Z pediocin mixture by pH-controlled mixed-strain batch cultures in supplemented whey permeate Goulhen, F.;Meghrous, J.;Lacroix, C. https://doi.org/10.1046/j.1365-2672.1999.00675.x
  19. Applied and Environmental Microbiology v.66 no.2 Bacteriocin production with Lactobacillus amylovorus DCE 471 is improved and stabilized by fed-batch fermentation Callewaert, R.;De Vuyst, L. https://doi.org/10.1128/AEM.66.2.606-613.2000
  20. J. Microbiol. Biotechnol. v.9 Identification and characterization of bacteriocin-producing lactic acid bacteria isolated from Kimchi Lee, H.J.;Park, C.S.;Joo, Y.J.;Kim, S.H.;Yoon, J.H.;Park, Y.H.;Hwang, I.K.;Ahn, J.S.;Mheen, T.I.
  21. Appl. Environ. Microbiol. v.64 Chemostat production of plantaricin C by Lactobacillus plantarum LL441 Bruno Barcena, J.M.;Sineriz, F.;De Llano, G.;Rodriguez, A.;Juan, E.S.
  22. Journal of Applied Microbiology v.88 no.3 Production of sakacin P by Lactobacillus sakei in a completely defined medium Moretro, T.;Aasen, I.M.;Storro, I.;Axelsson, L. https://doi.org/10.1046/j.1365-2672.2000.00994.x