Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
권호 표지

Development of Nuclear Ribosomal ITS DNA Marker for Tricholoma matsutake

Lee, Jae-Soon;Noh, Eun-Woon;Choi, Young-Im;Lee, Wi-Young;Kah, Kang-Hyeon

  • Published : 2004.06.30
⟨ Previous Next ⟩

Abstract

A PCR marker based on nuclear ribosomal intratranscribed spacer (ITS) DNA variation was developed to detect Tricholoma matsutake in both soil and pine root tissues. The nuclear ribosomal ITS regions of the mushroom were amplified, restriction digested and sequenced to standardize patterns for T. matsutake. The amplification products of other test samples including mycelium of T. matsutake, T. matsutake-like mushroom collected from pitch pine forest, pine roots and soil from pine mushroom forest were compared for restriction pattern. Based on ITS sequence, a new primer set was designed to specifically amplify T. matsutake DNA. The new primer set faithfully differentiated T. matsutake DNA from those of other samples. It was possible to detect T. matsutake from root tissue infected with the fungi and from soil sample taken from the forest where matsutake mushrooms were produced. The mushroom collected from pitch pine forest turned out to be T. matsutake

Keywords

References

  1. Crang, R. F. E. 1988. Artifacts in specimen preparation for scanning electron microscopy, In：Artifacts in Biological Electron Microscopy, pp. 107-129(Crang, R.F.E. and K.L. Klomparens, Eds.), Plenum Press, NY
  2. Crossley, A and D. Fowler. 1986. The weathering of scots pine epicuticular wax in polluted and clean air. New Phytologist 103：207-218 https://doi.org/10.1111/j.1469-8137.1986.tb00609.x
  3. Dickinson, S. 1969. Studies in the physiology of obligate parasitism. VI. Directed growth. Phytopathologische Zeitschrift 66：38- 49 https://doi.org/10.1111/j.1439-0434.1969.tb03084.x
  4. Green, F., M. Larsen and T. Highley. 1990. Ultrastructural morphology of the hyphal sheath of wood decay fungi modified by preparation for scanning electron microscopy. Biodeterioration Research 3：311-325
  5. Günthardt-Goerg, M. S., T. Keller, R. Matyssek and C. Scheidegger. 1994. Environmental effects on Norway spruce needle wax. European Journal of Forest Pathology 24：92-111 https://doi.org/10.1111/j.1439-0329.1994.tb01062.x
  6. Hanover, J. W and D. A. Reicosky. 1971. Surface wax deposits on foliage of Picea pungens and other conifers. American Journal of Botany 58：681-687 https://doi.org/10.2307/2441014
  7. Hansen, E. M. 1972. Germination behavior, vesicle formation by Cronartium ribicola and the infection of Pinus strobus. Ph. D. Thesis. University of Wisconsin, Madison. 118p
  8. Hansen, E. M and R. F. Patton. 1975. Types of germination and differentiation of vesicles by basidiospores of Cronartium ribicola. Phytopathology 65：1061-1071 https://doi.org/10.1094/Phyto-65-1061
  9. Hansen, E. M and R. F. Patton. 1977. Factors important in artificial inoculation of Pinus strobus with Cronartium ribicola. Phytopathology 67：1108-1112
  10. La, Y. J and C. K. Yi. 1995. Occurrence and management of Korean pine blister rust in Korea. Proceedings of the 4th IUFRO Rusts of Pines Working Party Conference. Tsukuba, Japan. p. 171-175
  11. Lanner, R. M. 1990. Biology, taxonomy, evolution, and geography of stone pines of the world. Pages 14-24 In W.C. Schmidt and K.J. McDonald, compilers. Proceedings-Symposium on whitebark pine ecosystems：ecology and management of a high-mountain resource. U.S. Department of Agriculture, Forest Service, Inter- mountain Research Station, general technical report INT-270, Ogden, Utah, USA
  12. Larsen, M. J and F. Green Ⅲ. 1992. Mycofibrillar cell wall extension in the hyphal sheath of Postia placenta. Canadian Journal of Microbiology 38：905-911
  13. Leyton, L and I.P. Armitage. 1968. Cuticle structure and water relations of the needles of Pinus radiata(D. Don). New Phytologist 67：31-38 https://doi.org/10.1111/j.1469-8137.1968.tb05451.x
  14. Leyton, L and B. E. Juniper. 1963. Cuticle structure and water relations of pine needles. Nature 198：770-771 https://doi.org/10.1038/198770a0
  15. McDonald, G. I and R. J. Hoff. 2000. Blister rust：An introduced plague. 'Whitebark pine communities：ecology and restoration' D.F, Tombeck, S.F. Arno, and R.E. Keane, editors. Island Press, Washington, D.C. USA
  16. Patton, R. F and R. N. Spear. 1978. Scanning electron microscopy of infection of Scotch pine needles by Scirrhia acicola. Phytopathology 68：1700-1704 https://doi.org/10.1094/Phyto-68-1700
  17. Patton, R. F and R. N. Spear. 1980. Stomatal influences on white pine blister rust infection. Phytopathologia Mediterranea 19：1-7
  18. Spaulding, P. C. 1922. Investigations of the white pine blister rust. USDA Bull. No. 957. 100p
  19. Spaulding, P. C. 1929. White pine blister rust：a comparison of European with North American conditions. USDA Technical Bulletin No. 87. 85p
  20. Todd, W. J. 1986. Effects of specimen prepa- ration on the apparent ultrastructure of micro- organism, In：Ultrastructure Techniques for icroorganism, pp. 87-100
  21. Trimble, J. L., J. M. Kelly, S. A. Tolin and D. M. Orcutt. 1982. Chemical and structural characterization of the needle epicuticular wax of two clones of Pinus strobus differing in sensitivity to ozone. Phytopathology 12：652- 656
  22. Woo, K.-S., L. Fins, G. I. McDonald and M. V. Wiese. 2001. Differences in needle mor- phology between blister rust resistant and susceptible western white pine stocks. Canadian Journal of Forest Research 31：1880-1886 https://doi.org/10.1139/cjfr-31-11-1880
  23. Woo, K.-S., L. Fins, G. I. McDonald, D. L. Wenny and A. Eramian. 2002. Effects of nursery environment on needle morphology of Pinus monticola Dougl. and implications for tree improvement programs. New Forests 24：113-129 https://doi.org/10.1023/A:1021230304530