Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Identification of New Microsatellite DNAs in the Chromosomal DNA of the Korean Cattle (Hanwoo)

  • Kim, J.W. (Institute of Biotechnology, Yeungnam University) ;
  • Hong, J.M. (Institute of Biotechnology, Yeungnam University) ;
  • Lee, Y.S. (School of Biological Resources, Yeungnam University) ;
  • Chae, S.H. (School of Biological Resources, Yeungnam University) ;
  • Choi, C.B. (Institute of Biotechnology, Yeungnam University) ;
  • Choi, I.H. (Institute of Biotechnology, Yeungnam University) ;
  • Yeo, J.S. (Institute of Biotechnology, Yeungnam University)
  • Received : 2003.10.02
  • Accepted : 2004.05.12
  • Published : 2004.10.01
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Abstract

To isolate the microsatellites from the chromosomal DNA of the Korean cattle (Hanwoo) and to use those for the genetic selection, four bacteriophage genomic libraries containing the chromosomal DNA of six Hanwoo steers showing the differences in meat quality and quantity were used. Screening of the genomic libraries using $^{32}P-radiolabeled 5'-({CA})_{12}-3$nucleotide as a probe, resulted in isolation of about 3,000 positive candidate bacteriophage clones that contain $(CA)_n$-type dinucleotide microsatellites. After confirming the presence of microsatellite in each positive candidate clone by Southern blot analysis, the DNA fragments that include microsatellite and flanking sequences possessing less than 2 kb in size, were subcloned into plasmid vector. Results from the analysis of microsatellite length polymorphism, using twenty-two PCR primers designed from flanking region of each microsatellite DNA, demonstrated that 208 and 210 alleles of HW-YU-MS#3 were closely related to the economic traits such as marbling score, daily gain, backfat thickness and M. longissimus dorsi area in Hanwoo. Interestingly, HW-YU-MS#3 microsatellite was localized in bovine chromosome 17 on which QTLs related to regulation of the body fat content and muscle ypertrophy locus are previously known to exist. Taken together, the results from the present study suggest the possible use of the two alleles as a DNA marker related to economic trait to select the Hanwoo in the future.

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References

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