Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
권호 표지

Significance and Detection of Latent Infection of Colletotrichum gloeosporioides on Strawberry

딸기탄저병균 Colletotrichum gloeosporioides 잠재감염주의 중요성 및 검출

Nam, Myeong-Hyeon;Lee, In-Ha;Gwon, Gyeong-Hak;Kim, Hong-Gi
남명현;이인하;권경학;김홍기

  • Published : 20040000
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Abstract

To investigate the primary inoculum for latent infection on strawberry (Fragaria×ananassa Duch.), healthy plants (HP) derived from tissue culture and symptomless local farm plants (LFP) were grown in a strawberry nursery field in Nonsan Strawberry Experiment Station and local farms from 2001 to 2002. Among HP, occurrence of anthracnose was firstly detected in 2.2% of daughter plants on July 10th (98 days after planting). Meanwhile, among LFP, 10% of mother plants were wilted on June 10th (68 days after planting). In local farms, anthracnose did not occur in the nursery field where HPs were planted, but anthracnose incidence in LFP were detected from 5 to 60%, based on the survey in Aug. 2001 and Sept. 2002. To detect rapidly latent infection of Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. on strawberry, 5 min ultraviolet light (UV) radiation, water rinse, 30 sec herbicide paraquat (2.5mL․L^^1) treatment, and 30 sec 70% ethanol treatment were tested, respectively. Acervulus formation of C. gloeosporioides was observed on petioles treated with either UV, water, paraquat, or ethanol. However, acervulus formation were not detected in non-treatment samples. Paraquat treatment was faster than other treatments for developing acervulus of C. gloeosporioides. Rapid and accurate detection of latent infection on strawberry anthracnose should be possible effectively by paraquat treatment on petioles.

딸기탄저병 1차 전염원으로서의 잠재감염주 역할을 조사하기 위해 2001년～2002년에 걸쳐 조직 배양된 건전묘와 병징이 없는 농가관행묘를 논산딸기시험장과 농가포장에서 재배하였다. 건전묘 가운데 탄저병 발생은 7월 10일(정식 후 98일)에 최초로 자묘에서 2.2% 발병한 반면 농가관행묘는 모주의 10%가 6월 10일(정식 후 68일)에 발병되었다. 2001년 8월과 2002년 9월에 농가포장의 탄저병 발생을 조사한 결과, 건전묘의 육묘포장에서는 발생하지 않았지만 농가관행묘 포장에서는 5～60%까지 검출되었다. 딸기식물체에서 탄저병균 Colletotrichum gloeosporioides(Penz.) Penz. & Sacc.의 잠재감염을 빠르게 검출하기 위해 UV 조사 5분, 물 세척, paraquat(2.5ml/L) 침지 30초, 70% 에탄올 침지 30초 처리하여 관찰하였다. C. gloeosporioides의 분생포자 형성은 UV, 물, paraquat, 에탄올 등의 처리에서 관찰되었으나 무처리에서는 관찰되지 않았다. paraquat처리는 C. gloeosporioides의 분생포자 형성이 가장 빨리 나타나 잠재감염주 검출에 효과적일 수 있다.

Keywords

References

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