Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effect of Methyl Jasmonate on in vitro Bulblet Formation and Enlargement from Shoot Clump of Allium victorialis

산마늘 다신초 덩어리로부터 인경 형성과 비대에 미치는 methy jjasmonate의 영향

  • Park, So-Young (Biotechnology Division, Korea Forest Research Institute) ;
  • Ahn, Jin-Kwon (Biotechnology Division, Korea Forest Research Institute) ;
  • Lee, Wi-Young (Biotechnology Division, Korea Forest Research Institute) ;
  • Park, Hae-Chin (Biotechnology Division, Korea Forest Research Institute)
  • 박소영 (국립산림과학원 생물공학과) ;
  • 안진권 (국립산림과학원 생물공학과) ;
  • 이위영 (국립산림과학원 생물공학과) ;
  • 박혜진 (국립산림과학원 생물공학과)
  • Published : 2004.03.01
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Abstract

The effect of abscisic acid (ABA) and methyl jasmonate (MeJA) on bulblet formation from the culture of Allium victorialis var. platyphyllum Makino was studied. Shoot clumps were cultured on MS medium containing ABA (0, 0.01∼2.0mg/L) and MeJA (0.01∼5.0mg/L). ABA at low concentrations (0.01mg/L) induced shoot proliferation without bulblet formation. However, bulblet formation started on the medium containing MeJA approximately in 4-6 weeks of culture. Furthermore, 1.0mg/L MeJA resulted in bulblet formation at high frequency (100％) as compare to the control (46.1％). Cortical cells of the bulblets enlarged on medium with MeJA had dense protein-like substance in expanded and round cells when examined under the microscope. The data described here show that formation and enlargement of bulblets from Allium victorialis can be improved by addition of appropriate concentration of MeJA.

산마늘의 다신초 덩어리로부터 인경형성을 위해 ABA와 MeJA가 첨가된 MS 배지에서 배양하였다. 저농도 (0.01mg/L)의 ABA처리구는 인경 형성보다 오히려 신초의 증식을 유도하였다. 인경은 MeJA처리구에서 배양 4∼6주 후 형성되기 시작하여 1.0mg/L MeJA 처리구에서 다신초 덩어리로부터 100% 인경형성이 이루어졌다. 조직학적 관찰 결과 생장조절제가 첨가되지 않은 배지의 인경과는 달리 MeJA처리구에서 비대된 인경의 외피세포는 크고 둥글며 단백질 등의 물질로 가득 차 있었다. 산마늘의 다신초 덩어리로부터 인경형성에 MeJA가 촉진적임을 알 수 있었다.

Keywords

References

  1. Creelman RA, Mullet JE (1997) Biosynthesis and action of jasmonates in plants. Annu Rev Plant Physiol Plant Mol Biol 48: 355-381 https://doi.org/10.1146/annurev.arplant.48.1.355
  2. Debeljak N, Regvar M, Dixon KW, Sivasithamparam K (2002) Induction of tuberisation in vitro with jasmonic acid and sucrose in an Australian terrestrial orchid, Pterostylis sanguinea. Plant Growth Reg 36: 253-260 https://doi.org/10.1023/A:1016570319387
  3. Han BH, Yae BW, Goo DH, Ko JY (1999) Effects of inorganic salts in MS medium, sucrose, and activated charcoal on bulblet formation from in vitro bulb scales in Lilium Oriental hybrid 'Casa Blanca'. J Kor Soc Hort Sci 26:103-107
  4. Haruki K, Yamada K, Hosoki T, Ohta K (1996) Effect of sugar and temperature on the growth of miniature bulblets of Lilium japonicum Thunb cultured on a rotary shaker. J Jap Soc Hort Sci 65: 363-371 https://doi.org/10.2503/jjshs.65.363
  5. Hays DB, Wilen RW, Sheng C, Molony MM, Pharis RP (1999) Embryo-specific gene expression in microspore-deiived embryos of Brassica napus. An interaction between abscisic acid and jasmonic acid. Plant Physiol 119:1065-1072 https://doi.org/10.1104/pp.119.3.1065
  6. Kastner U, Klahr A, Keller ERJ, Kahane R (2001) Formation of onion bulblets in uitro and viability during medium-term storage. Plant Cell Rep 20: 137-142 https://doi.org/10.1007/s002990000295
  7. Kim WB, Kim JG, Lee EA, Kim BH, Kim JK, Lim HT (1996) Plant regeneration from bulb explants of Allium uictorialis var. platyphyllum Makino. Korean J Plant Tiss Cult 23:123-127
  8. Koda Y, Kikuta Y (1991) Possible involvement of jasmonic acid in tuberization in Yam plants. Plant Cell Physiol 32: 629-633
  9. Koda Y (1997) Possible involvement of jasrnonates in various morphogenic events. Physiol Planta 100:639-946 https://doi.org/10.1111/j.1399-3054.1997.tb03070.x
  10. Kovac M, Ravnikar (1998) Sucrose and jasmonic acid interact in photosynthetic pigment metabolism and development of potato (solanum tuberosum L. cv. Sante) grown in vitro. Plant Growth Reg24:101-107 https://doi.org/10.1023/A:1005965626250
  11. Mita T, Shibaoka H (1983) Changes in microtubules in onion leaf sheath cells during bulb development. Plant Cell Physiol 24: 109-117
  12. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Planta 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  13. Niimi Y, Nakano M, Isogai N (1999) Effects of temperature and illuminating conditions on regeneration and development of bulblets in scale culture of seven Lilium spp. J Jap Soc Hort Sci 68: 28-34 https://doi.org/10.2503/jjshs.68.28
  14. Nojiri H, Yamane H, Seto H, Yamaguchi I, Murofushi N, yoshihara T, Shibaoka H (1992) Qualitative and quantitative analysis of endogenous jasmonic acid in bulbing and non-bulbing onion plants. Plant Cell Physiol 33:1225-1231
  15. Parthier B (1991) Jasmonates, new regulators of plant-growth and development - many facts and few hypothesis on their actions. Bot Acta 104:446-456 https://doi.org/10.1111/j.1438-8677.1991.tb00257.x
  16. Ravnikar M, Zel J, Plaper I, Spacapan A (1993) Jasmonic acid stimulates shoot and bulb formation of garlic in vitro. Plant Growth Reg 12: 73-77 https://doi.org/10.1007/BF00193236
  17. Reinbothe S, Reinbothe C, Lehmann J, Parthier B (1992) Differential accumulation of methyl jasmonate-induced mRNAs in response to abscisic acid and desiccation in barley (Hordeum uulgare). Physiol Plant 86:49-56 https://doi.org/10.1111/j.1399-3054.1992.tb01310.x
  18. Santos I, Salema R (2000) Promotion by jasmonic acid of bulb formation in shoot cultures of Narcissus triandrus L. Plant Growth Reg 30:133-138 https://doi.org/10.1023/A:1006381127884
  19. Van den Berg JH, Ewing EE (1991) Jasmonates and their role in plant growth and development, with special references to the control of potato tuberization: a review. Am Potato J 68: 781 -794 https://doi.org/10.1007/BF02853808
  20. Yamagishi M (1998) Effects of culture temperature on the enlargement, sugar uptake, starch accumulation, and respiration of in vitro bulblets of Lilium. japoncum Thumb. Sci Hort 73: 239-247 https://doi.org/10.1016/S0304-4238(97)00159-3
  21. Yamazaki H, Nishijima T, Yamato Y, Koshioka M, Miura H (1999) Involvement of abscisic acid (ABA) in bulb dormancy of Allium wakegi Araki I. Endogenous levels of ABA in relation to bulb dormancy and effects of exogenous ABA and fluridone. Plant Growth Reg 29:189-194 https://doi.org/10.1023/A:1006212427997
  22. Yeung EC (1999) The use of histology in the study of plant tissue culture systems-some practical comments, In Vitro Cell Dev Biol 35:137-143 https://doi.org/10.1007/s11627-999-0023-z
  23. Zel J, Debeljak N, Ucman R, Ravnikar M (1997) The effect of jasmonic acid, sucrose and darkness on garlic (Allium satiuum L.cv.ptujski jesenski) bulb formation in vitro, In Vitro Cell Dev Biol 33:231-235 https://doi.org/10.1007/s11627-997-0028-4

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