Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Cytotoxicity of Ethanol Extract of Raphanuse Sativus on a Human Lung Cancer Cell Line

인체 폐암 세포주에 대한 무의 에탄올 추출물의 세포독성

  • 임효빈 (호서대학교 식품생물공학전공 및 벤처전문대학원 첨단산업기술전공) ;
  • 이건순 (한국농업전문대학) ;
  • 채희정 (호서대학교 식품생물공학전공 및 벤처전문대학원 첨단산업기술전공)
  • Published : 2004.02.01
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Abstract

This study was desinged to investigate the cytotoxic effect of ethanonl extract of radish, Raphanuse sativus on human lung cancer cell lines (A-549) using MTT(3-〔4,5-dimethylthiazol-2-yl〕-2-yl)-2,5-diphenyltetrazolium bromide) assay. Radish trunk and radish root were extracted by a mixed solvent of water and ethanol (5 : 5, v/v) after drying, The extracts were used for anticancer activity assay. $IC_{50}$/ (50% inhibitory concentration) of radish trunk and radish root were 0.015% and 0.03%, respectively. Radish trunk extract showed higher anticancer activity than radish root extract at the same concentration of 0.01％.

무의 에탄올추출물을 이용하여 폐암에 대한 세포독성을 조사하였다. 청운 무를 무줄기와 무뿌리로 나누어 수세, 정선, 탈수한 후 에탄올과 물(5 : 5, v/v)의 흔합용매로 추출하고 폐암 세포주 A-549를 사용하여 MTT(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide)법을 사용하여 항암 활성을 분석하였다. 각 well의 570 nm에서의 흡광도를 측정하고 대조군의 흡광도에 대한 백분율을 산출하였다. 실험 결과 무줄기 추출물과 무뿌리 추출물은 모두 A-549에 대한 세포 독성을 가지고 있었다. 무줄기 추출물의 $IC_{50}$/(50% inhibitory concentration)은 0.015%이었고 무뿌리 추출물의 $IC_{50}$/은 0.03%이었다. 동일 농도(0.01%)에서 무줄기 추출물이 무뿌리 추출물보다 A-549에 대한 항암활성이 더 뛰어났다.

Keywords

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