Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Antioxidative Effects of Red Ginseng Saponins on Paraquat-induced Oxidative Stress

Paraquat 유도 산화적 스트레스에 대한 홍삼 사포닌의 항산화 효과

  • Published : 2004.03.01
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Abstract

This study was carried out to investigate the effect of the active ingredients from ginseng on paraquat(PQ) toxicity. Oxidative stress was induced by intraperitreatneal injection of PQ at a single dose of 25 mg/kg. Saponin treated groups were given protopanaxadiol saponins(PPD) or protopanaxatriol saponins(PPT)(5 mg/kg, orally) per day for 1, 3, & 7 days. We also investigated the relationship between lipid peroxidation and ginseng saponins by measuring the levels of superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase (GPx), reduced glutathione(GSH), malondialdehyde (MDA), and hydrogen peroxide(H$_2$O$_2$) in liver tissue. The activities of SOD, CAT, and GPx were generally high in the PPD group; the SOD activity on each day was the highest in the PPD group. The H$_2$O$_2$ content was the lowest in the PPD group. The GSH levels were significantly increased in the PPD. The levels of MDA(the end product of lipid peroxidation) were significantly lower in the red ginseng component groups than in the PQ group; the levels were especially low in the PPD groups. These results led us to conclude that the antioxidant effects of extracts from red ginseng prevent oxidative damage by direct antioxidant effects involving SOD, CAT, & GPx, and increasing the ability of the body to synthesize endogenous antioxidants.

본 연구에서는 PQ의 독성과 홍삼 성분의 항산화 활성성분을 알아보기 위해 생쥐를 이용하여 PQ 투여 후 총 사포닌, PPD, PPT성분을 1, 3, 7일간 경구 투여한 후, 간 조직에서의 SOD, CAT GPx의 활성과 GSH, MDA 및 과산화수소 함량등을 측정 비교함으로써 홍삼의 항산화활성 성분이 PQ 독성에 대한 회복 작용 그리고 지질과산화와의 상호 관련성을 검토하였다. 항산화 효소인 SOD, CAT GPx활성도는 전반적으로 PPD계가 높았고, SOD 활성은 PPD 투석군에서 전일에 걸쳐 가장 높은 활성을 보였다. 과산화수소의 함량은 PQ 투여군 기준으로 PPD 투여군에서 가장 낮은 결과를 보였다. 자유 라디칼에 의해 생성된 지질과산화의 최종산물인 MDA의 함량은 사포닌 투여군에서 유의성 있게 감소하였으며, 특히 PPD에서 다른 투여군들에 비해 현저한 함량 감소를 보였다. 이와 같이 사포닌의 항산화효과는 SOD, CAT및 GPx와 같은 항산화 효소의 직접적인 작용과 홍삼의 특정 성분들이 생체 내에서 내인성 항산화 물질의 합성능력을 강화시킴으로서 산화적 손상에 대한 회복작용을 향상시키는 결과로 생각된다.

Keywords

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