Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Molecular Differentiation of Bacillus spp. Antagonistic Against Phytopathogenic Fungi Causing Damping-off Disease

  • Cho, Min-Jeong (School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Young-Kwon (Institute of Korea Biotech) ;
  • Ka, Jong-Ok (School of Agricultural Biotechnology, Seoul National University)
  • Published : 2004.06.01
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Abstract

Gram-positive antagonistic bacilli were isolated from agricultural soils for possible use in biocontrol of plant pathogenic fungi, Fusarium oxysporum, Rhizoctonia solani, and/or Pythium ultimum. Among the 65 antagonistic Gram-positive soil isolates, 22 strains were identified as Bacillus species by 16S rDNA sequence analyses. Four strains, including DF14, especially exhibited multiple antagonistic properties against the three damping-off fungi. Genotypic properties of the Bacillus isolates were characterized by rapid molecular fingerprinting methods using repetitive extragenic palindromic-PCR (REP-PCR), ribosomal intergenic spacer-length polymorphisms (RIS-LP), 16S rDNA PCR-restriction fragment length polymorphisms (PCR-RFLP), and strain-specific PCR assays. The results indicated that the REP-PCR method was more valuable than the RIS-LP and 16S rDNA PCR-RFLP analyses as a rapid and reliable approach for bacilli typing and identification. The use of strain-specific primers designed based on 16S rDNA sequence comparisons enabled it to be possible to selectively detect a strain, DF14, which is being used as a biocontrol agent against damping-off fungi.

Keywords

References

  1. J. Mol. Biol. v.215 Basic local alignment tool Altschul,S.F.;W.Gish;W.Miller;E.W.Myers;D.J.Lipman https://doi.org/10.1016/S0022-2836(05)80360-2
  2. Adv. Microb. Ecol. v.4 The immunofluorescence approach in microbial ecology Bohlool,B.B.;E.L.Schmidt https://doi.org/10.1007/978-1-4615-8291-5_6
  3. Phytopathology v.89 Multiphasic analysis of xanthomonads causing bacterial spot disease on tomato and pepper in the Caribbean and Central America:Evidence for common lineages within and between countries Bouzar,H.;J.B.Jones;R.E.Stall;F.J.Louws;M.Schneider;J.L.W.Rademaker;F.J.de Bruijn;L.E.Jackson https://doi.org/10.1094/PHYTO.1999.89.4.328
  4. J. Clin. Microbiol. v.33 PCR amplification of rRNA intergenic spacer regions as a method for epidemiologic typing of Clostridum difficle Cartwright,C.P.;F.Stock;S.E.Beekmann;E.C.Williams;V.J.Gill
  5. J. Microbiol. Biotechnol. v.12 no.2 Molecular identification of vaginal Lactobacillus spp.isolated from Korean women Chang,C.E.;S.I.Pavlova;L.Tao;E.K.Kim;S.C.Kim;H.S.Yun;J.S.So
  6. Appl. Environ. Microbiol. v.59 Isolation and characterization of actinomycete antagonists of a fungal root pathogen Crawford,D.L.;J.M.Lynch;J.M.Whipps;M.A.Outsley
  7. Appl. Environ. Microbiol. v.58 Use of repetitive(repetitive extragenic palindromic and enterobacterial repetitive intergeneric consensus)sequences and the polymerase chain reaction to fingerprint the genomes of Rhizobium meliloti isolates and other soil bacteria de Bruijn,F.J.
  8. Appl. Environ. Microbiol. v.66 Use of repetitive DNA sequences and the PCR to differentiate Escherichia coli isolates from human and animal sources Dombek,P.;L.K.Johnson;S.T.Zimmerley;M.J.Sadowsky https://doi.org/10.1128/AEM.66.6.2572-2577.2000
  9. FEMS Microbiol. Lett. v.171 Biocontrol of plant disease:A (Gram-)positive perspective Emmert,E.A.B.;J.Handelsman https://doi.org/10.1111/j.1574-6968.1999.tb13405.x
  10. J. Gen. Microbiol. v.139 Typing of Clostridum difficile strains by PCR-amplification of variable length 16S-23S rDNA spacer regions Gurtler,V. https://doi.org/10.1099/00221287-139-12-3089
  11. Microbiology v.142 New approaches to typing and identification of bacteria using the 16S-23S rDNA spacer region Gurtler,V.;V.A.Stanisich https://doi.org/10.1099/13500872-142-1-3
  12. Plant Cell v.8 Biocontrol of soilborne plant pathogens Handelsman,J.;E.V.Stabb https://doi.org/10.1105/tpc.8.10.1855
  13. Phytopathology v.69 Control of Rhizoctonia solani on cotton seedlings with Pseudomonas fluorescens and with an antibiotic produced by the bacterium Howell,C.R.;R.D.Stipanovic https://doi.org/10.1094/Phyto-69-480
  14. J. Bacteriol. v.180 Novel division level bacterial diversity in a Yellowstone hot spring Hugenholtz,P.;C.Pitulle;K.L.Hershberger;N.R.Pace
  15. Appl. Environ. Microbiol. v.59 Use of repetitive sequences and the polymerase chain reaction technique to classify genetically related Bradyrhizobium japonicum serocluster 123 strains Judd,A.K.;M.Schneider;M.J.Sadowsky;F.J.de Bruijn
  16. Bacteriol. Rev. v.41 The peptide antibiotics of Bacillus:Chemistry,biogenesis, and possible role Katz,E.;A.L.Demain
  17. J. Clin. Microbiol. v.30 Molecular epidemiology of Pseudomonas cepacia determined by PCR ribotyping Kostman,J.R.;T.D.Edlind;J.J.LiPuma;T.L.Stull
  18. Nucleic Acid Techniques in Bacterial Systematics 16S/23S rRNA sequencing Lane,D.J.;E.Stackebrandt(ed.);M.Goodfellow(ed.)
  19. J. Microbiol. Biotechnol. v.12 no.2 Plant growth promoting Pseudomonas fluorescens GL20:Mechanism for disease suppression,outer membrane receptors for ferric siderophore,and genetic improvement for increased biocontrol efficacy Lim,H.S.;J.M.Lee;S.D.Kim
  20. Nucleic Acids Res. v.28 no.1 The RDP(Ribosomal Database Project)continues Maidak,B.L.;J.R.Cole;T.G.Lilburn;C.T.Parker Jr.;P.R.Saxman;J.M.Stredwick;G.M.Garrity;B.Li;G.J.Olsen;S.Pramanik;T.M.Schmidt;J.M.Tiedje https://doi.org/10.1093/nar/28.1.173
  21. Biol. Control. v.7 Biological control of gray mold in pears by antagonistic bacteria Mari,M.;M.Guizzardi;G.C.Pratella https://doi.org/10.1006/bcon.1996.0060
  22. J. Appl. Microbiol. v.89 Genotypic and phenotypic differentiation of an antifungal biocontrol strain belonging to Bacillus subtilis Marten,P.;K.Smalla;G.Berg https://doi.org/10.1046/j.1365-2672.2000.01136.x
  23. Microb. Ecol. v.28 Epiphytic bacteria antagonistic to Curvularia leaf spot of yam Michereff,S.J.;N.S.S.Silveira;A.Reis;R.L.R.Mariano
  24. Appl. Environ. Microbiol. v.64 Secondary metabilite-and endochitinase-dependent antagonism toward plant-pathogenic microfungi of Pseudomonas fluorescens isolates from sugar beet rhizosphere Nielsen,M.N.;J.Sorensen;J.Fels;H.C.Pedersen
  25. Appl. Environ. Microbiol. v.68 Genotypic and phenotypic diversity within species of purple nonsulfur bacteria isolated from aquatic sediments Oda,Y.;W.Wanders;L.A.Huisman;W.G.Meijer;J.C.Gottschal;L.J.Forney https://doi.org/10.1128/AEM.68.7.3467-3477.2002
  26. Appl. Environ. Microbiol. v.62 Use of repetitive intergenic DNA sequences to classify pathogenic and disease-suppressive Streptomyces strains Sadowsky,M.J.;L.L.Kinkel;J.H.Bowers;J.L.Schottel
  27. Appl. Environ. Microbiol. v.58 Isolation of 2,4-diacetylphloroglucinol from a fluorescent pseudomonad and investigation of physiological parameters influencing its production Shanahan,P.;D.J.O'Sullivan;P.Simpson;J.D.Glennon;F.O'Gara
  28. Adv. Appl. Microbiol. v.24 Recent chemical studies on peptide antibiotics from the genus Bacillus Shoji,J. https://doi.org/10.1016/S0065-2164(08)70640-3
  29. Manual of Methods for General Bacteriology Smibert,R.M.;N.R.Krieg;P.Gerhardt(ed.);R.G.E.Murray(ed.);R.N.Costilow(ed.);E.W.Nester(ed.);W.A.Wood(ed.);N.R.Krieg(ed.);G.B.Philips(ed.)
  30. Numerical Taxonomy Sneath,P.H.A.;R.R.Sokal
  31. PAUP:phylogenetic analysis using parsimony,version 4.0 Swofford,D.L.
  32. Methods Mol. Cell Biol. v.5 Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction Versalovic,J.;M.Schneider;F.J.de Bruijn;J.R.Lupski
  33. Appl. Environ. Microbiol. v.64 Genotypic characterization of Bradyrhizobium strains nondulating endemic woody legumes of the Canary islands by PCR-restriction fragment length plymorphism analysis of genes encoding 16S rRNA(16S rDNA)and 16S-23S rDNA intergenic spacers,repetitive extragenic palindromic PCR genomic fingerprinting, and partial 16S rDNA sequencing Vinuesa,P.;J.L.W.Rademaker;F.J.de Bruijn;D.Werner
  34. Annu. Rev. Phytophthol. v.26 Biological control of soilborne plant pathogens in the rhizosphere with bacteria Weller,D.M. https://doi.org/10.1146/annurev.py.26.090188.002115