KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Quantification of Tocopherol and Tocotrienol Content in Rice Bran by Near Infrated Reflectance Spectroscopy

근적외선분광분석기를 이용한 미강의 Tocopherol과 Tocotrienol 함량 분석

  • Published : 2004.06.01
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Abstract

Near infrared reflectance spectroscopy (NIRS) is a rapid and accurate analytical method for determining the composition of agricultural products and feeds. This study was conducted to determine tocopherol and tocotrienol contents in rice bran by using NIRS system. Total 80 rice bran samples previously analyzed by HPLC were scanned by NIRS and over 60 samples were selected for calibration and validation equation. A calibration equation calculated by MPLS(modified partial least squares) regression technique was developed and coefficient of determination for tocopheyol and tocotyienol content were 0.975 and 0.984, respectively, in calibration sets. Each calibration equation was fitted to validation set that was performed with the remaining samples not included is the calibration set, which showed high positive correlation both in tocopherol and tocotrienol content file. This results demonstrate that the developed NIRS equation can be practically used as a rapid screening method for quantification of tocopherol and tocotrienol contents in rice bran.

미강에 함유되어 있는 토코페롤 및 토코트리에놀의 함량을 비파괴적으로 신속하게 추정하기 위하여 NIRS(근적외선 분광분석기)를 이용한 분석 방법을 검토하였다. 벼 유전자원 80계통의 미장을 사용하여 HPLC에서 분석된 토코페롤 및 토코트리에놀의 함량치를 NIRS 스펙트럼에 적용시킨 후 검량식을 작성하였다. NIRS의 검량식을 몇가지 방법에 의하여 비교 분석한 결과 2차 미분된 스펙트럼을 MPLS(Modified Partial Least Squares)를 이용한 회귀식에 이용하는 것이 가장 적합하였다. HPLC를 이용한 유전자원들의 성분 함량과 NIRS에서 도출된 검량식과의 상관계수는 토코페롤과 토코트리에놀이 각각 0.992, 0.953을 나타내었다. 이들 검량식은 validation file 에서도 0.846 및 0.956의 높은 상관을 보여 미강 상태에서 토코페롤 및 토코트리에놀의 함량을 NIRS를 이용하여 신속하게 분석할 수 있을 것으로 판단되었다.

Keywords

References

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