KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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The Effects of Extracts from Ginseng, Wormwood and Pine needle in Pulmonary Structure and Anti-oxidant Enzyme in Smoking

흡연 시 인삼, 쑥, 솔잎 추출물이 폐 세포의 구조와 항산화 효소에 미치는 영향

  • Jung, Hyuk (Department of Biochemistry, Kyungpook National University) ;
  • Kim, Sang-Ki (Department of Biochemistry, Kyungpook National University) ;
  • Kim, Sang-Guk (Department of Biochemistry, Kyungpook National University) ;
  • Sung, Mi-Young (Department of Biochemistry, Kyungpook National University) ;
  • Kim, Hyun-Jung (Department of Biochemistry, Kyungpook National University) ;
  • Kim, Bum-Hak (Department of Biochemistry, Kyungpook National University) ;
  • Kim, Yu-Young (Department of Biochemistry, Kyungpook National University)
  • 정혁 (경북대학교 자연과학대학 생화학과) ;
  • 김상기 (경북대학교 자연과학대학 생화학과) ;
  • 김상국 (경북대학교 자연과학대학 생화학과) ;
  • 성미영 (경북대학교 자연과학대학 생화학과) ;
  • 김현정 (경북대학교 자연과학대학 생화학과) ;
  • 김범학 (경북대학교 자연과학대학 생화학과) ;
  • 김유영 (경북대학교 자연과학대학 생화학과)
  • Published : 2004.04.01
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Abstract

Cigarette smoke causes atypical structure of pulmonary (cell structural) and oxidative damage. Therefore, we carried out to determine if exposure to cigarette smoke alters pulmonary structure and anti-oxidant related enzyme in a ICR mice model, when natural product extracts using by manual sprayer. The mice were divided into five groups, control group, sham-treated group (Sham), natural product extracts-treated group (NPE), natural product extracts-treated with smoke-exposed group (NPE-SM) and smoke-exposed (SM) group. All groups are similar to control group in weight, but SM group is lower than the other groups. Microscopic image of the pulmonary structure in SM group showed deleterious alterations in the morphology, but the other groups are maintained in original structure. In anti-oxidant related enzyme, SOD (superoxide dismutase) and catalase, SM group represents the lowest enzyme activity among all groups. These results indicate that the natural product extracts is an efficient tissue protective agent against smoke-induced lung injury.

본 실험에서 메탄올을 추출 용매로 한 인삼, 쑥, 그리고 솔잎 추출물을 수동분무기를 이용하여 호흡을 통해 직접 실험동물에게 투여하였을 경우 그 효과가 폐의 구조를 유지하는 데 도움을 줄뿐만 아니라 항산화 효소에도 길항적인 효과를 나타냄을 알 수 있었다. 즉, 추출물을 흡입시킴으로서 세포막의 지질과산화를 억제하여 세포의 구조와 기능을 유지함으로써 흡연으로 인한 세포의 파괴 및 불규칙적인 구조 이상을 줄일 뿐만 아니라 추출물에 포함되어 있는 인삼의 saponin, 쑥의 sesquiterpene과 cineol, 솔잎의 rutin 등의 항산화제의 작용에 의해 흡연으로 생긴 유해 라디칼의 분해를 촉진시키거나 항산화 효소의 활성을 증가시킴으로써 폐의 정상적인 기능에 도움을 주는 것으로 생각된다. 특히, 지금까지 연구되어진 in vitro나 in vivo에서의 경구투여나 주사를 통한 방법이 아니라 호흡을 통해 직접 폐에 투입함으로써 그 결과가 빠르게 나타날 수 있을 것으로 생각된다.

Keywords

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