Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

Effects of Benincasa hispida Seeds Intake on Blood Glucose and Lipid Levels in Streptozotocin Induced Diabetic Rats

동과 종자의 섭취가 Streptozotocin 유발 당뇨 흰쥐의 혈당 및 혈장 지질수준에 미치는 영향

  • 임숙자 (덕성여자대학교 자연과학대학 식품영양학과) ;
  • 김예리 (덕성여자대학교 자연과학대학 식품영양학과)
  • Published : 2004.05.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was designed to examine the effects of diets containing different levels of seeds of Benincasa hispida (wax-gourd)on plasma glucose, insulin levels and lipid profiles in streptozotocin (STZ)-induced diabetic rats. Sprague-Dawley rats were induced diabetes mellitus by STZ injection (45 mg/kg) into the tail vein and were divide into 4 groups: normal, STZ-control, two experimental groups. Normal and STZ-control groups were fed an AIN-93 diet and 2 experi-mental groups were fed a modified diet containing 2.5% and 5.0% of wax gourd seed powder for 4 weeks. The weight gain and feed efficiency ratio in 5.0% seed groups were higher than in STZ-control group. A significant lowering effects of plasma glucose levels were observed in the wax gourd seed 2.5% and 5.0 groups. The plasma insulin level did not differ after treatment with wax gourd seed in experimental rats. The intake of 2.5% wax gourd seed did not result in sig-nificant change in plasma cholesterol levels compared to diabetic control group. The intake of 2.5% wax gourd seeds had normalized the increased levels of plasma triglyceride and free fatty acids after 4 weeks. In 5% wax gourd seed group, cholesterol, triglyceride and free fatty acid levels were elevated compared to 2.5% wax gourd seed group. The results of the present study indicate that 2.5% wax gourd seed group would be more effective to control the diabetes than 5% wax gourd seed group.

본 연구는 220 g 내외의 Sprague-Dawley계 수컷 흰쥐를 이용하여 정상군, 당뇨대조군, 동과 종자 2.5% 섭취군 및 동과 종자 5.0% 섭취군으로 구분하여 실험하였다. 동과 종자의 첨가량을 각각 2.5%와 5.0%로 달리하여 4주 동안 섭취시킨 후 혈장 중의 포도당과 지질함량에 미치는 영향을 검토하였다. 당뇨대조군에 비해 동과 종자 5.0%를 섭취한 군에서는 4주 후의 체중이 초기에 비하여 7.4% 증가함을 볼 수 있었다. 혈당 수준은 실험 1주째부터 당뇨대조군에 비해 당뇨실험군이 유의적으로 감소하기 시작하여 실험 4주 후에는 유의적으로 감소되었다. 혈장 콜레스테롤 수준은 동과 종자 5.0%를 섭취한 군에서 실험 1주째부터 유의적으로 낮아지기 시작하여 실험 4주째까지 그 수준을 유지하였다. 혈장 중의 HDL -cholesterol, 중성지방 및 유리지방산 수준은 정상군에 비해 당뇨대조군에서 유의적으로 높게 나타났고, 혈장 중성지방 수준에서는 당뇨대조군에 비해 종자 2.5% 섭취군에서 유의적으로 낮게 나타났다. AST 활성도는 정상군과 당뇨유발군간의 유의적인 차이가 나타나지 않았고, ALT 활성도는 정상군에 비해 당뇨대조군에서 유의적으로 높게 나타났다. 이상의 실험결과 동과 종자 2.5%와 5.0%의 섭취가 streptozotocin 유발 당뇨 쥐에서 혈당강하 효과를 보였을 뿐만 아니라 2.5% 섭취에서는 지질함량을 저하시키는 효과도 있는 것으로 보여졌다.

Keywords

References

  1. Cancer Res (suppl) v.43 Inhibition of neoplasia by minor dietary constituents Wattenberg,L.W.
  2. Proc Nutr Soc v.49 Inhibition of carcinogenesis by minor constituents of the diet Wattenberg,L.W. https://doi.org/10.1079/PNS19900022
  3. Korean J Mycol v.12 Studies on constituents of the higher fungi of Korea. An antitumor fraction from the culture filtrate of Lentinus edodes DMC7 Chung,S.K.;Choi,E.C.;Kim,B.K.
  4. The Korean Nutr Soc v.34 no.1 Effect of nutrition education on diabetic management in diabetic patients Lim,H.S.;Chun,J.H.;Kim,Y.S.;Nam,M.S.
  5. Top Clin Nutr v.3 Source and amount of dietarys mellitus Coluston,A.M.;Hollenbeck,C.B.
  6. Diabetes Care v.4 Lipid disorders in diabetics Goldberg,R.B. https://doi.org/10.2337/diacare.4.5.561
  7. Am J Med v.83 Abnormal lipoprotein metabolism in noninsulin dependent diabetes mellitus Reaven,K.M.
  8. Diabetes Care v.6 The role of circulating glucose and triglyceride concentrations and their interaction with other risk factors as determinations of arterial disease in nine deabetic. Population samples from the WHO multinational study West,K.M.;Ahnja,M.M.;Bennet,P.H. https://doi.org/10.2337/diacare.6.4.361
  9. Korean J Nutr v.30 no.1 The effect of Trichosantes kirilowii Max. subfractions on the insulin activity in streptozotocin induced diabetic rats and their acute toxicity Lim,S.J.;Choi,S.S.
  10. Vegetables of Korea Lee,W.S.
  11. The Encyclopedia Britannica of Korea
  12. Handbook of Oriental Medicine Huh,J.
  13. J. Ethnopharmacology v.78 Extracts of Benincasa hispida prevent development of experimental ulcers Grover,J.K.;Adiga,G.;Vats,V.;Athi,S.S. https://doi.org/10.1016/S0378-8741(01)00334-8
  14. Encyclopedia of Chinese Medicine Lee,K.S.;Ahn,D.K.;Shin,M.K.;Kim,C.M.
  15. Korean J Nutr v.36 no.4 Effects of Benincase hispida intake on blood glucose and lipid levels in streptozotocin induced diabetic rats Lim,S.J.;Jeong,J.G.;Kim,M.W.;Choi,S.S.;Han,H.K.;Park,J.E.
  16. J. Nutr v.127 Components of the AIN-93 diets as improvements in the AIN-76A diet Reeves,P.G.
  17. Cancer Chemother Rep v.29 Studies on the diabetogenic actions of streptozotocin Rakieten,N.;Rakieten,M.L.;Nadkarni,M.V.
  18. J. Clin Invest v.48 Diabetogenic action of streptozotocin: Relationship of dose to metabolic response Junod,A.;Lambert,A.E.;Stauffacher,W.;Renod,A.E. https://doi.org/10.1172/JCI106180
  19. Diabetologia v.27 Mechanism of streptozotocin-induced and alloxaninduced damage in rat β-cells Wilson,G.L. https://doi.org/10.1007/BF00276973
  20. Diabetes v.29 Amino acid transport in isolated hepatocytes from steptozotocin diabetic rats Samson,M. https://doi.org/10.2337/diabetes.29.12.996
  21. Proc Soc Exp Biol Med v.126 Studies of the diabetogenic action of streptozotocin Junod,A.;Lambert,A.E.;Orci,L.;Picet,R.;Gonet,A.E.;Renold,A.E.
  22. Duksung Bull Pharm Sci v.2 Pharmacological studies on the water extract of fractus of Lycium chinese Mill Lee,S.S.;Kim,J.W.
  23. Pharmacol Rev v.22 Drugs producing diabetes through damage of the insulin secreting cells Rerup,C.C.
  24. Scandiv J Lab Invest v.12 On the enzymatic determination of blood glucose Raabo,E.;Terkildsen,T.C.
  25. J Clin Endocrinol Metab v.33 Use of polyethylene glycol to separate free and antibody-bound peptide hormones in radioimmunoassays Desbuquois,B.;Aurbach,G.B. https://doi.org/10.1210/jcem-33-5-732
  26. Clin Chem v.20 Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in werum Richimond,W.
  27. J Clin Chem v.20 Enzymatic determination of total serum cholesterol Allain,C.C.;Poon,L.S.;Chan,C.S.G.;Richmond,W.;Paul C Fu
  28. Clin Chem v.24 Cholesterol in high-density lipoprotein: Use of $Mg^{2+}$/dextran sulfate in its measurement Finely,P.R.;Schifman,R.B.;Williams,R.J.;Luchti,D.A.
  29. J Clin Chem v.21 Serum triglyceride determined colorimetry with an enzyme that produces hydrogen peroxide Giegel,J.L.;Ham,S.B.;Clema,W.
  30. A summary of clinical tested.(revised ed.) v.29 Kim,J.C.
  31. Am J Clin Pathol v.28 A colorimetric method the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase Reitman,S.;Frankel,S.
  32. Eur J Nutr v.121 The effect of fish oil on triglycerides, cholesterol, fibrinogen and malondialdehyde in human supplemented with vitamin Haglund,O.;Loustarines,R.;Wallin,R.;Wibell,I.;Sladeen,T.
  33. PC_STAT. dept. Food Sci Univ. Georgia Rao,M.;Blane,K.;Zonnenberg,M.
  34. J Biol Chem v.249 Effect of streptozotocin diabetes and insulin treatment on the rate of protein synthesis in tissues of the rat in vivo Pain,V.M.;Garlick,P.
  35. Korean J Nutr v.34 Effects of soy oligosaccharides on blood glucose and lipid metabolism in streptozotocin-induced diabetic rats Kim,M.H.;Kim,H.Y.;Kim,W.K.;J.Y.;Kim,S.H.
  36. Endocrinol v.119 Phenylethanolamin N-methyltransferase in the basis of STZ diabetic rats Fisher,K.J.;Stewart,J.K. https://doi.org/10.1210/endo-119-6-2586
  37. Diabetes v.40 Regulation of glucose metabolism in livers and kidneys of NOD mice Sococher,M.;Kunjara,S.;Baquer,N.Z.;Mclean,P. https://doi.org/10.2337/diabetes.40.11.1467
  38. Diabetes v.38 Vasopressin in rats with genetic and streptozotocin-induced diabetes Brooks,D.P.;Nutting,D.F.;Crofton,J.L.;Share,L. https://doi.org/10.2337/diabetes.38.1.54
  39. Diabetes v.39 Cholesterol metabolism in alloxan-induced diabetic rabbits O'Meara NMG;Devery RAM;Owens,D.;Collins,P.B.;Johson,A.H.;Tomkin,G.H. https://doi.org/10.2337/diabetes.39.5.626
  40. Korean J Nutr v.30 no.7 Effects of mushroom proteinbound polysaccharides ion blood glucose level and energy metabolism in streptozotocin-induced diabetic rats Kim,M.W.;Park,M.H.;Kim,G.H.
  41. Metabolism v.22 Plasma triglyceride transport kinetics in diabetes mellitus Nikkila,E.A.;Kekki,M. https://doi.org/10.1016/0026-0495(73)90024-3
  42. J Korean Soc Food Nutr v.20 Effects of nicotinamide on the serum lipid composition in streptozotocin-induced diabetic rats Choi,J.W.;Sohn,K.H.;Kim,S.H.
  43. Diabetes v.45 Hematocrit and risk of NIDDM Wannamethee,S.G.;Perry,I.J.;Shaper,A.G. https://doi.org/10.2337/diabetes.45.5.576