Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

The Effects on Antimicrobial and Cytotoxicity of Brassica oleracea L. Fractions.

적채 분획물의 항균, 암세포 증식 억제효과

  • 박윤자 (신라대학교 식품영양학과, 마린-바이오 산업화 지원센타) ;
  • 전광혜 (신라대학교 식품영양학과, 마린-바이오 산업화 지원센) ;
  • 김선희 (부산대학교 의과대학 생화학교) ;
  • 배송자 (신라대학교 식품영양학과, 마린-바이오 산업화 지원센타)
  • Published : 2004.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to investigate the affects on antimicrobial and cytotoxicity of red cabbage (Brassica oleracea L.,BO). In the paper disc test, antimicrobial activity of BO fractions was increased in proportion to its concentration. Among the various solvent fractions of methanol extract (BOM) of BO, the ethylacetate partition layer (BOMEA) showed the strongest antimicrobial activity We also determined the cytotoxicity and Quinone Reductase induced effect of BO extract and fractions on human cancer cells. The cytotoxicity of BO fractions on HepG2, HeLa and MCF-7 cells was evaluated by MTT assay. The BOMEE and BOMEA showed strong cytotoxic effects on all cancer cell lines we used. The quinone reductase induced effect of BO fractions on HepG2 cells, the hexane partition layer (BOMH) at a dose of 200 $\mu{g}$/ml was 2.88 times more effective compared to the control values of 1.0.

노화억제, 항균, cholesterol 저하작용 등 다양한 생리활성을 가지고, 기관지염, 천식 등 호흡기 질환에도 옛부터 효과가 있는 것으로 알려져 있으며 식품으로 애용되고 있는 적채를 metanol (BOM)로 먼저 추출하고 이를 hexane 분획물(BOMH), ethyl ether 분획물(BOMEE), ethyl acetate 분획물(BOMEA), butanol 분획물(BOMB) 및 물 분획물(BOMA) 등 다섯가지의 각 용매별로 분획하여 적채의 항균, 암세포 증식 억제 및 QR유도 효과를 연구하였다. 먼저 적채의 각 분획물을 Streptococcus mutans, Bacillus subtilis, Escherichia coli. Pseudomonas aeruginosa 및 Aspergil-lus oryzae의 5가지 균주에 첨가시료농도를 증가시키면서 첨가하였다. 즉, 각 분획 별 시료를 500, 1000, 1500 및 2000 $\mu{g}$/ml의 농도로 사용균주에 각각 처리하였을때 BOMEA에서 비교적 높은 항균 활성 효과를 나타내었고, 그 다음으로는 BOMEE이었다. 적채의 암세포 증식억제 효과(cytotoxicity)를 MTT assay 로 실험한 결과, 3종의 암세포주 HepG2, HeLa 및 MCF-7은 모두 BOMEE에서 높은 암세포 증식억제 효과를 보였으며, HepG2 세포주를 이용한 암예방 QR 유도 활성은 다른 분획층에 비해 비극성 용매층인 BOMH과 BOMEE에서 유의적으로 QR유도 활성을 증가시키는 것으로 나타났다. 이 결과를 기초로 독특한 보라색을 띄며 식탁에서 널리 애용되고 있는 자주색 대표식품인 적채의 항균 및 암세포 성장저지 및 QR 유도 효과를 일으키는 생리활성 물질의 존재와 기전 규명에 유익한 자료가 될 것으로 사료된다.

Keywords

References

  1. Bissett, D. L., J. R. Chatter and D. P. Hannon. 1991. Chronic ultraviolet radiation-induced increases in skin iron and the photopotentive effect of topically applied iron chelators. Photochem, photobiol. 54, 215-223 https://doi.org/10.1111/j.1751-1097.1991.tb02009.x
  2. Bae, S. J. 2002. The effects on antimicrobial and anticarcinogenic activity of momordica charantia L., The Korean Journal of Natrition 35, 880-885
  3. Carmichael, J., W. G. De Graff, A. F. Gazder, J. D. Minna and J. B. Mitchell. 1987. Evalution of a tetrazolium based semiautomated colorimetric assay : assessment of chemosensitivity testing. Cancer Res 47, 936-942
  4. Choi, J. S., S. H. Park and I. S. Kim. 1989. Studies on the active principles of wild vegetables on biotransformation of drug. Kor. J. Parmacogn 20, 117-122
  5. Chun, H. J., E. Y. Choi, S. C. Yoon, J. W. Nam, S. H. Beak and W. Woo, 2001. Inhibitory effects of ethanol extract of atractylodis rhizoma alba on melanin biosynthesis. Yakhak Hoeji 45, 269-275
  6. Davidson, P. M. and M. E. Parish. 1989. Methods for testing the efficacy of food antimicrobials. Food Technol January: 148-155
  7. John, T. P., S. S. Sharad, S. S. Mohammad, T. T. Robert and C. Yogesh. 1998. Mechanisms of anticarcinogenic properties on curcumin ; the effect of curcumin on glutathione linked detoxification enzymes in rat liver. The international. J. Biochem. and Cell Biology 30, 445-456 https://doi.org/10.1016/S1357-2725(98)00015-6
  8. Kim, J. D. and J. H. Kim. 1999. Development of anti- oxidant tea for anti-aging. Bull. Yosu Nat'l. Univ 14, 401- 409
  9. Kim, Y. D., S. K. Kang and O. J. Choi. 2001. Antimicrobial activity of coriander (coriandrum sativum L.) exreact. J. Korean Soc. Food Sci. Nitr 30, 692-696
  10. Kwack, S. N., 1991. Studies on salt tolerace of vegetables, bulletin of institute of littoral biota. Mokpo National University 8, 51-64
  11. Lee, S. M., S. H. Rhee and K. Y. Park. 1997. Antimutagenic effect of various cruciferous vegetables in Salmonella assaying system. J. Fd Hyg. Safety 12, 321-327
  12. Lee, S. M. and S. H. Rhee. 1997. Inhibitory effect of various Cruciferous vegetables on the growth of human cancer cells. Korean J. Life Science 7, 234-240
  13. Michael, C., Alley, Dominic A Scudiero, Anne Monks, Miriam L. Hursey, Maciej J. Czerwinski, Donald L. Fine, Betty J. Abbortt, Joseph G. Mayo, Robert H. Shoemaker and Michael R. Boyd 1988. Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Res 48, 589-601
  14. Micozzi, M.S. and J. A. Tangrea. 1989. General introduction : Relation for the nutritional prevention of cancer. In 'Nutrition and cancer prevention', Moon, T.E. and Micozzi, M.S. (ed), Marcel Dekker. Inc., New York. 3
  15. Ong, T. M., W. Z. Whong, S. Stewart and H. E. Brockman. 1986. Chlorophyllin; a potent antimutagen against environmental and dietary complex mixture. Mutat. Res 173, 111-115 https://doi.org/10.1016/0165-7992(86)90086-2
  16. Park, B. J., H. S. Suh, G. S. Chung and J. K. Sohn 1987. Studies on protoplast culture and fusion in cruciferae. Korean J. Breed 19, 230-234
  17. Park, H. J., 1998. Induction of quinone reductase and its regulatory mechanism at the transcriptional level by Scutellaria baicalensis, Ph. D. Dissertation, Yonsei University
  18. Park, Y. J., M. H. Kim and S. J. Bae. 2002. Enhancement of anticarcinogenic effect by combination in lycii fructus with vitamin C. J. Korean Soc. Food Sci. Nutr 31, 143-148 https://doi.org/10.3746/jkfn.2002.31.1.143
  19. Prochaska, H. J. and A. B. Santamaria, 1988. Direct measurement of NAD(P)H : Quinone reductase from cells cultured in microtiter wells : A screening assay for anticarcinogenic enzyme inducers. Anal, Biochem. 169, 328-336 https://doi.org/10.1016/0003-2697(88)90292-8
  20. Shim, S. M., S. W. Choi. and S. J. Bae. 2000. Effects of quinone reductase induction and cytotoxicity of the angelica radix extracts. J. Korean Soc. Food Sci. Nutr 29, 147-152
  21. Yang, M. J., M. G. Kim, S. J. Lim, H. S. Ann, and R. M. Ahn. 1999. Inhibitory effects of water-acetone extracts of chestnut inner shell, pine needle and hop on the melanin biosynthesis. Yakhak Hoeji 43, 494-501

Cited by

  1. Effect of Growth Inhibition and Quinone Reductase Activity Stimulation of Makgeoly Fractions in Various Cancer Cells vol.37, pp.3, 2008, https://doi.org/10.3746/jkfn.2008.37.3.288