YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Activity of Glutathione S-transferase and Effect of Alcohol Decomposition on the Fruit of Hovenia dulcis Thunb

지구자의 Glutathione S-transferase 활성과 알코올 분해효과

  • Cha, Bae-Cheon (Department of Bio-industry and technology, Sangji University) ;
  • Lee, Eun-Hee (Department of Bio-industry and technology, Sangji University) ;
  • Lee, Eun (Department of Bio-industry and technology, Sangji University) ;
  • Park, Hang-Ho (Hanoeul Food CO., LTD.)
  • 차배천 (상지대학교 생명자원과학대학 생명산업학과) ;
  • 이은희 (상지대학교 생명자원과학대학 생명산업학과) ;
  • 이은 (상지대학교 생명자원과학대학 생명산업학과) ;
  • 박행호 ((주)한얼식품)
  • Published : 2004.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

For the determination of glutathione S-transferase activity, a new method was established by using HPLC system. Moreover, amount of enzyme for a optimum reaction was determined by a comparative study with a variety concentration of enzyme. Using a established method, activity of glutathione S-transferase that is alcohol metabolizing enzyme was investigated on the fruit of Hovenia dulcis Thunb. As the result of experiment, EtOH and $H_2O$ extracts of the fruit of Hovenia dulcis Thunb showed visible a synergistic effect of glutathione S-transferase activity. On a continuous experiment, EtOH and $H_2O$ extracts of the fruit of Hovenia dulcis Thunb showed alcohol decomposition activity on the in vivo test using rat. These results suggest that the fruit of Hovenia dulcis Thunb may be useful in the prevention of hangover.

Keywords

References

  1. Park, S. M., Kang, B. K. and Chung, T. H. : The effect of mildronate on serum alcohol concentration and hangover syndrome. J Korean Soc. Food Sci. Nutr. 27, 168 (1998)
  2. 이정균 : 알코올 및 알코올 중독, 정신의학 18장, 일조각, 서울 p. 390 (1989)
  3. Kim, C. I. : Cause and effect of hangover. Food Industry and Nutrition. 4, 26 (1999)
  4. Kim, D. J., Kim, W., Yoon, S. J., Choi, B. M., Kim, J. S., Go, H. J., Kim, Y. K. and Jeong, J. S. : Effects of alcohol hangover on cytokine production in healthy subjects. Alcohol. 31, 167 (2003) https://doi.org/10.1016/j.alcohol.2003.09.003
  5. Devaney, M., Graham, D. and Greeley, J.: Circadian variation of the acute and delayed response to alcohol: investigation of core body temperature variations in humans. Pharmacol. Biachem. Behav. 75, 881 (2003)
  6. Swift, R. and Davidson, D. : Alcohol hangover mechanisms and mediators. Alcohol Health Res. World. 22, 54 (1998).
  7. Trevisan, L. A., Boutros, N., Petrakis, I. L. and Krystal, J. H. : Complications of alcohol withdrawal, Pathophysiological insights. Alcohol Health Res. World. 22, 61 (1998)
  8. Lieber, C. S. : Medical disorders of alcoholism. N. Engl. J. Med. 333 , 1058 (1995) https://doi.org/10.1056/NEJM199510193331607
  9. Park, S. C., Han, J. G., Han, J. A. and Park, Y. C. : Aspartate decrease lipid peroxidation and protein carbonylation in liver of chronic ethanol-fed rats. Korean J. Biochem. 21, 145 (1994)
  10. Armstrong, R. N. : Structure, catalytic mechanism and evolution of the glutathione transferases. Chem. Res. Taxicol. 10, 2 (1997) https://doi.org/10.1021/tx960072x
  11. Habig, W. H., Pabst, M. J. and Jakoby, W. B. : Glutathione S-transferases. J Biol. Chem. 249, 7130 (1974)
  12. Zang, K. and Wong, P. : Inhibition of the effluxof glutathione S-conjugates by plant polyphenols. Biochem. Phannacol. 52, 1631 (1996) https://doi.org/10.1016/S0006-2952(96)00570-9
  13. Jungyakdesajon : Sohakkyan, Sanghae Science Publ. Co., Sanghae, p. 413 (1985)
  14. Kim, T.J. : Korean resources plants, Seoul National University Press, Seoul, Korea, p. 72 (1996)
  15. Kim, O. K. : Protective effects of extracts of Hovenia dulcis Thunb on hepatotoxicity in carbon tetrachloride intoxicated rats. J. Korean Soc. Food Sci. Nutr. 30, 1260 (2001)
  16. Hase, K., Ohsugi, M., Xiong, Q., Basnet, P., Kadota, S. and Namba, T. : Hepatoprotective effect of Hovenia dulcis Thunb on experimental liver injuries induced by carbon tetrachloride or D-galactosamine/lipopolysaccharide. BioI. Pharm. Bull. 20, 381 (1997)
  17. Cho, J. Y., Moon, J. H. and Park, K. H. : Isolation and identification of 3-methoxy-4-hydroxybenzoic acid and 3-methoxy-4-hydroxycinnamic acid from hot water extracts of Hovenia dulcis Thunb and confirmation of their antioxidative and antimicrobial activity. Korean J. Food Sci. Technol. 32，1403 (2000)
  18. Yoshikawa, M., Murakami, T., Ueda, T., Yoshizumi, S. and Ninomiya, K. : Bioactive constitutents of chinese natural medicines. III. Absolute stereo structures of new dihy-droflavonoids, Hovenitins I. II and III, Isolated from Hovenia duleis Thunb : Inhibitory effect on alcohol-induced muscular relaxation and hepatoprotective activity. Yakugaku Zasshi. 117, 108 (1997)
  19. Lee, M. K., Kim, Y. G., An, S. W., Kim, M. H., Lee, J. H. and Lee, H. Y. : Biological activities of Hovenia dulcis Thunb. Korean J. Medicinal Crop Sci. 7, 185 (1999)
  20. Stoelting, M. S. and Tjeerdema, R. S. : Glutathione-dependent biotrans- formation of 1-chloro-2,4-dinitrobenzene in arterial and venous blood of the striped bass (Marone saxitilis). Aquat. Toxicol. 50, 177 (2000) https://doi.org/10.1016/S0166-445X(99)00107-1
  21. Song, M. R., Choe, S. N. and Park，K. H. : Glutathione S-transferase activity and its changes to chemical pollution in edible shells and fishes. Korean J. Food Sci. Technol. 30，206 (1998)
  22. Chang, M., Zhang, F, Shen, L., Pauss, N., Alam, I., Breemen , R. B. van, Blond, S. Y. and Bolton, J. L. : Inhibition of glutathione S-transferase activity by the quinoid metabolites of equine estrogens. Chem. Res. Toxicol. 11, 758 (1998) https://doi.org/10.1021/tx9702190
  23. Ishikawa, T., Kobayashi, K., Sogame, Y. and Hayashi, K. : Evidence for leukotriene C4 transport mediated by an ATP-dependent glutathione S-conjugate carrier in rat heart and liver plasma membranes. FEBS Lett. 259, 95 (1989) https://doi.org/10.1016/0014-5793(89)81503-0
  24. Cha, B. C., Lee, E. H. and Cho, J. Y. : Glutathione S-transferase, activity and hyaluronidase inhibitory effect of medicinal plants. Kor. J Pharmacogn. 35, in press (2004)
  25. Igarashi, T. and Kitagawa, H. : Glutathione S-transferase, Tokuseiseigagaku I, Chichin Shokan, Tokyo p. 298 (1989)
  26. Na, C. S., Chung, N. C., Yang, K. H., Kim, S. H., Chung, H. S. and Dong, M. S. : Hepatoprotective and blood alcohol lowering effects of fruit peduncle estract of Hovenia dulcis var. Koreana in the in vitro and in vivo animal models. Yarhar Hoeji 48, 34 (2004)