The Korean Journal of Ecology

The Ecological Society of Korea (한국생태학회)
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Spatial Genetic Structure and Genetic Diversity of a Rare Endemic Juniperus chinensis var. sargentii in Mt. Halla, Korea

희귀식물인 눈향나무(Juniperus chinensis var. sargentii)의 공간분포에 따른 유전구조 및 유전적 다양성

  • Choi, Hyung-Soon (Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Hong, Kyung-Nak (Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Chung, Jae-Min (Korea National Arboretum) ;
  • Kim, Won-Woo (Department of Forest Genetic Resources, Korea Forest Research Institute)
  • Published : 2004.10.01
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Abstract

Juniperus chinensis var. sargentii Henry is a short and creeping evergreen shrub which reaches about 50㎝ in height and occurs in the northeast Asia and in high mountains over the South Korea. Its distribution is restricted, and the number of individuals are gradually decreasing. This study was conducted to estimate spatial pattern, genetic diversity and spatial genetic structure of J. chinensis var. sargentii. A total of 131 clumps were studied in the study area (40m × 60m). The spatial pattern of this population was random (Aggregation index R=1.031). In spite of the small number and the limited distribution, the level of genetic diversity (Shannon's index 1=0.463) was relatively high as compared with those of other plant species with similar ecological characteristics. ISSR genotypes of all individuals were investigated to find the genetic relationship of clumps and genets. Fifteen clumps were composed to be clones, and a total of 116 unique genotypes were composed by separate genets. Spatial autocorrelation analysis using Tanimoto distance showed that the genetic patch was established within 8m. The effect of clonal reproduction on genetic structure was almost nothing.

눈향나무(Juniperus chinensis var. sargentii Henry)는 사할린, 일본 등의 동북아시아와 한반도에서는 전국의 고산지대에서 자라는 상록성 소관목으로서 희귀 및 멸종 위기 식물(산림청)로 지정되어 있다. 본 연구에서는 한라산에 서식하는 눈향나무 집단의 분포형태 및 특성, 유전적 다양성과 공간적 유전구조를 파악하였다. 조사구(40m × 60m)에는 총 131개의 눈향나무 clump가 자생하고 있었으며 이들 전체 본수를 대상으로 군집지수를 계산한 바, 임의분포하고 있음을 확인하였다 모든 개체들에 대하여 ISSR 유전자형을 비교한 결과, 15개체는 클론으로, 나머지는 각기 서로 다른 genet으로 구성 되 어 있었다. 21개 의 ISSR 표지자를 바탕으로 계산한 Shannon의 다양성지수(I=0.463)는 적은 개체수와 제한적 분포에도 불구하고 유전적 다양성이 다른 수종에 비해 비교적 높은 편으로 나타났다. 공간적 자기상관 분석방법을 이용하여 공간적 유전구조를 파악한 결과, 조사지역내의 눈향나무 집단은 8m 이내에서 유전적으로 유사한 군락구조를 갖고 있는 것으로 나타났으며, 클론 번식이 공간적 유전 구조에 미치는 영향은 거의 없는 것으로 판단되었다.

Keywords

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