Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Inhibitory Effect of Ginseng Polysaccharides on Rotavirus Infection

  • Published : 2004.02.01
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Abstract

Polysaccharides and saponins were isolated from the root of Panax ginseng C.A. Meyer (Family Araliaceae), treated at various temperatures, and their inhibitory effects on rotavirus were investigated. As the temperature of processing increased, the molecular weight of the polysaccharides decreased, but the yields of water extracted increased. These polysaccharides inhibited rotavirus infection in MA104 cells, but there were no significant differences in rotavirus infection-inhibitory potency. However, ginseng saponins did not exhibit rotavirus infection-inhibitory activity.

Keywords

References

  1. Albert, M. J. and R. F. Bishop. 1984. Cultivation of human rotavirus in cell culture. J. Med. Virol. 13: 377-383
  2. Bae, E. A., M. J. Han, N. I. Baek, and D. H. Kim. 2001. In vitro anti-Helicobacter pylori activity of panaxytriol isolated from ginseng. Arch. Pharm. Res. 24: 297-299
  3. Bae, E.-A., M. J. Han, M.-J. Song, and D.-H. Kim. 2002. Purification of rotavirus infection-inhibitory protein from Bifidobacterium breve K-110. J. Microbiol. Biotechnol. 12: 553-556
  4. Bae, E. A., M. J. Han, M. K. Choo, S. Y. Park, and D. H. Kim. 2002. Metabolism of 20(S)- and 20(R)-ginsenoside R$_g3$ by human intestinal bacteria and its relation to in vitro biological activities. Biol. Pharm. Bull. 25: 58-63
  5. Bae, E. A., M. K. Choo, E. K. Park, H. Y. Shin, and D. H. Kim. 2002. Metabolism of ginsenoside R$_c$ by human intestinal bacteria and its related antiallergic activity. Biol. Pharm. Bull. 25: 743-747
  6. Bae, E. A., N. Y. Kim, M. J. Han, and D.-H. Kim. 2003. Transformation of ginsenosides to compound K (IH-901) by lactic acid bacteria of human intestine. J. Microbiol. Biotechnol. 13: 9-14
  7. Belogortseva, N. I., J. Y. Yoon, and K. H. Kim. 2000. Inhibition of Helicobacter pylori hemagglutination by polysaccharide fractions from roots of Panax ginseng. Planta Med. 66: 217-220
  8. Bishop, R. F. 1993. Development of candidate rotavirus vaccines. Vaccine 11: 247-254 https://doi.org/10.1016/0264-410X(93)90025-S
  9. Cukor, G. and N. R. Blacklow. 1984. Human viral gastroenteritis. Microbiol. Res. 48: 157-179
  10. Flores, J. and A. Z. Kapokian. 1992. Rotavirus, pp. 255- 288. In R. W. Ellis (ed.), Vaccines: New Approaches to Immunological Problems. Butterworth- einemann press, Boston
  11. Kim, K. H., I. S. Suh, J. M. Kim, C. W. Kim, and Y. J. Cho. 1989. Etiology of childhood diarrhea in Korea. J. Clin. Microbiol. 27: 1192-1196
  12. Kim, Y. S., K. S. Kang, and S. I. Kim. 1990. Study on antitumor and immunomodulating activities of polysaccharide fractions from Panax ginseng: Comparison of effects of neutral and acidic polysaccharide fraction. Arch. Pharm. Res. 13: 330-337
  13. Kim, N. D., S. Y. Kang, J. H. Park, and V. B. Schini-Kirth. 1999. Ginsenoside R$_g3$ mediates endothelium-dependent relaxation in response to ginsenosides in rat aorta: role of K+ channels. Eur. J. Pharmacol. 367: 41-49
  14. Kim, W. Y., J. M. Kim, S. B. Han, S. K. Lee, N. D. Kim, M. K. Park, J. K. Kim, and J. H. Park. 2000. Steaming of ginseng at high temperature enhances biological activity. J. Nat. Prod. 63: 1702-1704
  15. Kim, D.-H., M.-J. Song, E.-A. Bae, and M. J. Han. 2000. Inhibitory effect of herbal medicines on rotavirus infectivity. Biol. Pharm. Bull. 23: 356-358
  16. Konno, T., H. Suzuki, A. Imai, T. Kutsuzawa, N. Ishida, N. Katsushima, M. Sakamoto, S. Kitaoka, R. Tsuboi, and M. Adachi. 1978. A long term survey of rotavirus infection in Japanese children with acute gastroenteritis. J. Infec. Dis. 138: 569-576
  17. Lee, S. J., J. H. Sung, S. J. Lee, C. K. Moon, and B. H. Lee. 1999. Antitumor activity of a novel ginseng saponin metabolite in human pulmonary adenocarcinoma cells resistant to cisplatin. Cancer Lett. 144: 39-43
  18. Lee, J.-C., K. Na, J.-M. Yun, and J.-K. Hwang. 2001. In vitro bifidodogenic effect of nondigestible oligosaccharides isolated from red ginseng Marc. J. Microbiol. Biotechnol. 11: 858-862
  19. Smith, E. M., M. K. Esters, D. Y. Graham, and C. P. Gerba. 1979. A plaque assay for the simian rotavirus SAII. J. Gen. Virol. 3: 513-519
  20. Tanaka, N., O. Tanaka, and S. Shibata. 1972. Chemical studies on the oriental plant drugs. XXVIII. Saponins and sapogenins of ginseng; Stereochemistry of sapogenin of ginsenoside Rb1, Rb2 and Rc. Chem. Pharm. Bull. 20: 1212-1216
  21. Wakabayashi, C., H. Hasegawa, J. Murata, and I. Saiki. 1998. In vivo antimetastatic action of ginseng protopanaxadiol saponins is based on their intestinal bacterial metabolites after oral administration. Oncol. Res. 9: 411-417
  22. Woods, P. A., J. Gentsch, V. Gouvea, L. Mata, A. Simhon, M. Santosham, Z. S. Bai, and S. Urasawa. 1992. Distribution of serotype of human rotavirus in different populations. J. Clin. Microbiol. 30: 781-785