Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Electrochemical Property of Immobilized Spinach Ferredoxin on HOPG Electrode

  • Nam Yun-Suk (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Kim, You-Sung (Department of Chemistry, Sogang University) ;
  • Shin, Woon-Sup (Department of Chemistry, Sogang University) ;
  • Lee, Won-Hong (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Choi, Jeong-Woo (Department of Chemical and Biomolecular Engineering, Sogang University)
  • Published : 2004.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

The stability and electrochemical properties of a self-assembled layer of spinach ferredoxin on a quartz substrate and on a highly oriented pyrolytic graphite electrode were investigated. To fabricate the ferredoxin self-assembly layer, dimyristoylphosphatidylcholine was first deposited onto a substrate for ferredoxin immobilization. Surface analysis of the ferredoxin layer was carried out by atomic force microscopy to verify the ferredoxin immobilization. To verify ferredoxin immobilization on the lipid layer and to confirm the maintenance of redox activity, absorption spectrum measurement was carried out. Finally, cyclic-voltammetry measurements were performed on the ferredoxin layers and the redox potentials were obtained. The redox potential of immobilized ferredoxin had a formal potential value of -540 mV. It is suggested that the redox-potential measurement of self-assembled ferredoxin molecules could be used to construct a biosensor and biodevice.

Keywords

References

  1. Armstrong, F. A., P. A. Cox, H. A. O. Hill, V. J. Lowe, and B. N. Oliver. 1987. Metal ions and complexes as modulators of protein-interfacial electron transport at graphite electrodes. J. Electroanalytical Chem. 217: 331- 366.
  2. Choi, H. G., B. K. Oh, W H. Lee, and J. W. Choi. 2001. Deposition behavior and photoelectrochemical characteristics of chlorophyll a Langmuir-Blodgett films. Biotechnol. Bioprocess Eng. 6: 183- 188.
  3. Choi, J. W., W. Lee, J. M. Cho, Y. K. Kim, S. Y. Park, and W H. Lee. 2002. Control of fed rate using neurocontroller incorporated with genetic algorithm in fed batch cultivation of Scutellaria baicalensis Georgi. J. Microbiol. Biotechnol. 12(4): 687-691.
  4. Choi, J. W., Y. S. Nam, and M. Fujihira. 2004. Nanoscale fabrication of biomolecular layer and its application to biodevices. Biotechnol. Bioprocess Eng. 9(2): 76- 85.
  5. Crispin, D. J., G. Street, and J. E. Varey. 2001. Kinetics of the decomposition of [2Fe-2S] ferredoxin from spinach: Implications for iron bioavailability and nutritional status. Food Chem. 72: 355- 362.
  6. Dijk, C., T. Eijs, J. W Leeuwen, and C. Veeger. 1984. Direct electron transfer between a chemically, viologen-modified glassy-carbon electrode and ferredoxins from spinach and Megasphaera elsdenii. FEBS Lett. 166: 76- 80.
  7. Halagdjian, J., I. Thierry-Chef, and P. Bianco. 1996. Permselective-membrane pyrolytic graphite electrode for the study of microvolumes of [2Fe-2S] ferredoxin. Talanta 43: 1125-1130.
  8. Kievit, O. and G. W Brudvig. 2001. Direct electrochemistry of photosystem I.J. Electroanaiytical Chem. 497: 139-149.
  9. Lehninger, A. L., D. L. Nelson, and M. M. Cox. 1993. Principles ofBiochemistry, pp. 547. 2nd Ed. Worth Publishers, New York, U.S.A.
  10. Nassar, A.-E. F., J. F. Rusking, M. Tominaga, J. Yanagimoto, and N. Nakashima. 1996. Electrochemistry of cast films of ferredoxin and lipid bilayers on electrodes. J. Electroanalytical Chem.416: 183- 185.
  11. Oh, B. K, Y. K. Kim, Y. M. Bae, W H. Lee, and J. W Choi. 2002. Detection of Escherichia coli O157:H7 using immunosensor based on surface plasmon resonance. J. Microbiol. Biotechnol. 12(5): 780- 786.
  12. Otsuka, S. and T. Yamanaka. 1988. Metalloproteins, pp. 154- 155. Kodansha Ltd., Tokyo.
  13. Rusling, J. F. and A.-E. F. Nassar. 1993. Enhanced electron transfer for myoglobin in surfactant films on electrodes. J. Am. Chem. Soc. 115: 11891.
  14. Verhagen, M., T. A. Link, and W. R. Hagen. 1995. Electrochemical study of the redox properties of [2Fe-2S] ferredoxin: Evidence for superreduction of the rieske [2Fe-2S] cluster. FEBS Lett. 363: 75-78. https://doi.org/10.1016/0014-5793(95)00283-F