Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Selection of Transgenic Potato Plants Expressing NDP Kinase 2 Gene with Enhanced Tolerance to Oxidative Stress

NDP Kinase 2 유전자를 도입한 산화스트레스 내성 형질전환 감자의 선발

  • 탕리 (한국생명공학연구원 식물세포공학연구실) ;
  • 권석윤 (한국생명공학연구원 환경생명공학연구실) ;
  • 윤대진 (경상대학교 대학원 응용생명과학부) ;
  • 곽상수 (한국생명공학연구원 환경생명공학연구실) ;
  • 이행순 (한국생명공학연구원 식물세포공학연구실)
  • Published : 2004.09.01
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Abstract

Arabidopsis NDPK2 (AtNDPK2) is a key singaling component that regulate cellular redox state and known to enhance multiple stress tolerance when over-expressed in Arabidopsis plant (Moon et al. 2003). In order to develop transgenic potato plants with enhanced tolerance to multiple stresses, we placed an AtNDPK2 cDNA under the control of a stress-inducible SWPA2 promoter or enhanced CaMV 35S promoter. Transgenic potato plants (cv. Superior and Atlantic) were generated using an Agrobacterium-mediated transformation system and selected on MS medium containing 100 mg/L kanamycin. Genomic Southern blot analysis confirmed the incorporation of AtNDPK2 cDNA into the potato genome. When potato leaf discs were treated with methyl viologen (MV) at 10 $\mu$M, transgenic plants showed higher tolerance to MV than non-transgenic or vector-transformed plants. The NDPK2 transgenic potato plants will be further used for analysis of stress-tolerance to multiple environmental stresses.

복합스트레스 내성 유전자 NDP kinase 2 유전자를 도입시킨 형질전환 감자를 개발하기 위하여 이 유전자를 산화스트레스에 의해 발현이 강하게 유도되는 SWPA2 프로모터 또는 enhanced CaMV 35S 프로모터에 연결한 벡터를 제작한 후 각각 Agrobacterium 매개로 형질전환 하였다. 기관발생 경로에 의해 kanamycin 저항성 식물체를 재분화 시킨 후 Southern 분석으로 외래 유전자가 안정적으로 감자 게놈내로 삽입되었음을 확인하였다. 형질전환 감자 식물체의 잎 조직을 대상으로 10 $\mu$M methyl viologen에 대한 내성 검정을 조사하여 산화스트레스 내성 형질전환 감자 식물체를 2 개체씩 선발하였다. 선발된 식물체는 건조, 고온 등의 여러 가지 환경스트레스 내성 분석을 실시할 예정이며 이로부터 복합재해에 내성을 지닌 감자 품종을 개발할 수 있을 것으로 기대한다.

Keywords

References

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  2. Production of Transgenic Birdsfoot trefoil Plants by Introduction of 'SWPA2 Promoter + AtNDPK2 Gene' vol.25, pp.4, 2005, https://doi.org/10.5333/KGFS.2005.25.4.281
  3. Enhanced tolerance of transgenic potato plants overexpressing nucleoside diphosphate kinase 2 against multiple environmental stresses vol.17, pp.4, 2008, https://doi.org/10.1007/s11248-007-9155-2