Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effects of Membrane Filter and Sucrose Concentrations on the Growth of Balloon Flower (Platycodon grandiflorum A. DC.) Plantlets In Vitro

Membrane Filter와 Sucrose 농도가 도라지(Platycodon grandiflorum A. DC.) 기내 배양묘의 생장에 미치는 영향

  • 최소라 (전라북도농업기술원 진안숙근약초시험장) ;
  • 김명준 (전북대학교 농업과학기술연구소) ;
  • 은종선 (전북대학교 원예학과 생물산업연구소) ;
  • 안민실 (전라북도농업기술원 남원고냉지화훼시험장) ;
  • 임회춘 (전라북도농업기술원 남원고냉지화훼시험장) ;
  • 류정 (전라북도농업기술원 진안숙근약초시험장) ;
  • 유동현 (전라북도농업기술원)
  • Published : 2004.09.01
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Abstract

The shoots of balloon flower (Platycodon grandiflorum A. DC.) in vitro germinated from seeds were cultured on MS basal medium containing 0.1 mg/L NAA under the various sucrose concentrations and with/without membrane filter (MF) on the lid of vessel. The growth responses were checked to obtain healthy plantlets. The $CO_2$ and $C_2$H$_4$ concentration in vessel without MF were higher than those with MF. The $CO_2$ concentration without MF was increased as days in culture went by whereas the $C_2$H$_4$ concentration was decreased. The plant growth with MF and high sucrose concentration was good. Fresh and dry weight of plantlets cultured in sucrose 4.5% with MF were higher than those in no sucrose without MF. Also the content of chlorophyll of plantlets cultured with MF was high and the content of sugar was shown a similar results and a remarkable difference between MF treatments, especially. Stomata cultured with MF was closer than that without MF and mesophyll of leaf were more developed with MF or in high sucrose concentration. When the plantlets were transplanted in the pot at 25$\pm$2$^{\circ}C$, 75% relative humidity and low PPFD (photosynthetic photon flux density), the percentage of survival after 13 days without MF was 0% but it was 100% with MF regardless of sucrose concentrations.

기내 배양환경을 조절하여 건전한 유식물체를 획득하기 위해 도라지를 무균발아시켜 shoot부분을 NAA 0.1 mg/L가 첨가된 MS 기본배지에 치상한 후 membrane filter 부착 유무와 sucrose 농도를 달리 처리하여 생장반응을 조사하였다. Membrane filter 미부착구에서는 배양용기 내의 $CO_2$와 $C_2$H$_4$ 농도가 membrane filter 부착구에 비해 높았으며 membrane filter가 부착되지 않았을 때 $CO_2$ 농도는 배양기간이 경과될수록 증가하였으나 $C_2$H$_4$ 농도는 감소하는 경향이었다. 식물체 생장은 membrane filter 부착구가 미부착구에 비해 양호하였고, 미부착구에서는 sucrose 농도가 증가할수록 생육이 양호하게 나타났다. 생체중과 건물중은 membrane filter를 부착하지 않은 sucrose 4.5% 처리구에 비해 membrane filter를 부착한 sucrose 무첨가구에서 비슷하거나 높았다. 엽록소 함량은 membrane filter 부착구에서 미부착구에 비해 높았으며 당 함량도 비슷한 경향이었는데 두 처리간 차이는 더 컸다. 기공은 membrane filter 부착구에서 미부착구에 비해 닫혀 있었으며 엽육조직은 membrane filter를 부착하거나 sucrose 농도가 높을수록 잘 발달하였다. 또한 상토에 이식한 후 13일째 생존율은 sucrose 처리 농도에 관계없이 membrane filter 미부착구는 0%인데 비해 부착구는 100%로 나타났다.

Keywords

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Cited by

  1. The Growth Response of Balloon Flower (Platycodon grandiflorum A. DC.) Plantlets In Vitro as Affected by Air Exchanges and Light Intensity vol.32, pp.1, 2005, https://doi.org/10.5010/JPB.2005.32.1.023
  2. Plant Regeneration via Adventitious Shoot Formation in Platycodon grandiflorum (Jacq. A. DC.) vol.44, pp.3, 2017, https://doi.org/10.5010/JPB.2017.44.3.330