Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Micropropagation of Philodendron wend-imbe through Adventitious Multi-bud Cluster Formation

다아체 형성에 의한 Philodendron Wend-imbe의 대량번식

  • Published : 2004.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

In order to micropropagate uniform plantlets of Philodendron wend-imbe, the shoot tips were cultured on media supplemented with 0.5-10.0mg/L BA or 0.01-1.0 mg/L thidiazuron (TDZ). The multi-bud clusters from basal part of shoots formed vigorously on media containing 5.0-10.0 mg/L BA or 0.05-0.1 mg/L TDZ. Shoot formation from the bud cluster sections (5-7mm) was achieved favorably on medium with 5.0mg/L BA and 20 g/L sucrose. Lowering of sucrose in medium to 20 g/L was effective for the inhibition of callus growth from basal part of shoots. Growth of shoots and their rooting were favorable on media containing 1.0-2.0 mg/L IBA or 0.1mg/L NAA. The rooted plantlets were acclimatizated effectively in soil mixed with perlite 1: peat moss 1 or peat moss alone.

본 실험은 Philodendron wend-imbe를 기내배양하여 일시에 균일한 식물체를 대량생산하기 위하여 실시하였다. Philodendron의 경정에서 다아체 형성은 BA 5.0-10.0mg/L 또는 TDZ 0.05-0.1 mg/L가 첨가된 MS 배지에서 양호하였다. 형성된 다아체 절편체 (5-7mm)를 BA 5.0 mg/L와 sucrose 20g/L가 첨가된 MS 배지에서 배양한 결과, 신초 및 다아체의 증식이 매우 양호하였으며, 신초기부에서 callus 발생이 억제되었다 다아체 절편체에서 신초의 발생 및 발근은 IBA 1.0-2.0mg/L 또는 NAA 0.1 mg/L가 첨가된 배지가 효과적이었으며, 발근된 신초의 순화는 perlite와 peat moss의 1:1 혼합용토 또는 peat moss가 적합하였다.

Keywords

References

  1. Earle ED, Langhans RW (1974) Propagation of Chrysanthemum in vitro. I. Multiple plantlets from shoot tips and the establishment of tissue culture. J Amer Soc Hort Sci 99: 128-132
  2. Fellman CD, Read PE, Hosier MA (1987) Effect of thidiazuron and CPPU on meristem formation and shoot proliferation. HortScience 22: 1197-1200
  3. Hackett WP (1985) Juvenility, maturation and rejuvenation in wood plants. Hort Rev 7: 109-158
  4. Han BH, Joung HY, Ko JY (1997) In vitro propagation of Ficus benjamina by shoot tip culture. J Kor Soc Hort Sci 38: 315-319
  5. Han BH, Yae BW, Yu HJ, Shin JS (2001) In vitro propagation of Spathiphyllum floribundum cv. Cupid. Korean J Plant Tiss Cult 28: 209-213
  6. Kusey WE, Hammer PA, Weiler TC (1980) In vitro propagation of Gypsophila paniculata L. `Bristol Fairy'. HortScience 15: 600-601
  7. Murashige T, Skoog F (1962) A revised medium for rapid growth and biossays with tobacco tissue culture. Physiol Plant 15: 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  8. Pennazio S (1975) Effect of adenine and kinetin on development of carnation tips cultured in vitro. J Hort Sci 50: 161-164
  9. Pierik RLM (1990) Commercial micropropagation in Europe. In: Debergh P, Zimmerman R (eds), Micropropagation, Kluwer Academic Publishers, Dordrecht, pp 155-166
  10. Reynolds JF (1987) Chemical regulation in tissue culture; An overview. HortScience 22: 1192-1194
  11. Schnapp SR, Preece JE (1986) In vitro growth reduction of tomato and carnation microplants. Plant Cell Tiss Org Cult 6: 3-8 https://doi.org/10.1007/BF00037752
  12. Takayama S, Misawa M (1982) Regulation of organ formation by cytokinin and auxin in Lilium bulbscales grown in vitro. Plant Cell Physiol 23: 67-74 https://doi.org/10.1093/oxfordjournals.pcp.a076331
  13. Ziv M, Arial T (1991) Bud proliferation and plant regeneration in liquid-cultured Philodendron treated with ancymidol and paclobutrazol. J Plant Growth Regul 10: 53-57 https://doi.org/10.1007/BF02279311
  14. Ziv M, Yogev T, Krebs O (1986) Effects of paclobutrazol and chlomequat on growth pattern and shoot proliferation of normal and variant Aechmea faciata `Baker' plants regenerated in vitro. Israel J Bot 35: 175-182

Cited by

  1. In vitro Mass Propagation of Ardisia pusilla DC. vol.32, pp.4, 2005, https://doi.org/10.5010/JPB.2005.32.4.281