Transactions of the Korean Society of Mechanical Engineers A (대한기계학회논문집A)

The Korean Society of Mechanical Engineers (대한기계학회)
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Study on PDMS/Class Microthermostat Fabrication and Evaluation for Restriction Enzyme Reaction

제한효소 반응용 PDMS/유리 마이크로 항온조 제작 및 특성평가

  • 진석호 (한양대학교 대학원 정밀기계공학과) ;
  • 조용진 (한양대학교 대학원 정밀기계공학과) ;
  • 안유민 (한양대학교 기계공학과, 마이크로바이오칩센터)
  • Published : 2004.10.01
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Abstract

In this paper, we report a microthermostat using PDMS (poly-dimethylsiloxane) and glass. This PDMS/glass chip is able to maintain constant temperature that is necessary for restriction enzyme reaction. Since PDMS is the low-cost and the mass-producible material and has very good biochemical compatibility, PDMS chip has more benefit than general Si chip. Heater was made of Au wiring patterned on Pyrex glass. A reaction chamber has a capacity of about 3 ${mu}ell$. We performed a restriction enzyme reaction by using the fabricated microthermostat and conventional method. Then, with the electrophoresis, we made a comparison between the result from the micro reactor and the one from conventional method.

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References

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