Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

Effects of Benincasa Hispida Seed Supplementation on Glyeogen status and Lipid Peroxidatin in Streptozotecin-Induced Diabetic Rats

동과인의 보충이 당뇨 유발 흰쥐의 글리코겐과 지질과산화에 미치는 영향

  • 김명화 (덕성여자대학교 자연과학대학 식품영양학과)
  • Published : 2004.12.01
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Abstract

This study was designed to examine the effects of diets containing different levels of seeds of Benincasa hispida(wax gourd) on glycogen, protein levels and lipid profiles as well as malondialdehyde (MDA) in streptozotocin (STZ)-induced diabetic rats. Sprague-Dawley rats were induced diabetes mellitus by STZ injection (45 mg/kg) into the tail vein and were divided into four groups: normal, STZ-control, and two experimental groups. Normal and STZ-control groups were fed the AIN-93 diet and the two experimental groups were fed a modified diet containing 2.5% and 5.0% of wax gourd seed powder for four weeks. The liver, muscle, lung, kidney, and pancreas were excised after sacrifice, then the glycogen, protein, and lipid peroxidation products were measured. The rats fed 2.5% wax gourd seed group showed higher levels of liver glycogen compared with that of the STZ-control group. The levels of kidney protein were significantly increased in the 2.5% and 5.0% wax gourd seed groups. There were no significant difference cholesterol and liver triglyceride levels of the liver and MDA concentration in the liver, lung, and kidney among all four groups. These results show that wax gourd seed treatment of 2.5% and 5.0% doses did not exhibit profound anti-lipid peroxidation properities.

Keywords

References

  1. Cho NH. Prevention of type II diabetes: overview of diabetes prevention trial. J Korean Diabetes Assoc 26: 26-37,2002
  2. Lim HS, Chun JH, Kim YS, Nam MS. Effect of nutrition education on diabetic management in diabetic patients. Korean Nutr Soc 34: 69-78, 2001
  3. King H, Zimmet P, Raper LR, Balkau B. The natural history of impaired glucose tolerance in the Micronecian population of Nauru: a six-year follow-up study. Diabetologia 26: 39-43, 1984
  4. Vessal M, Hemmati M, Vasei M. Antidiabetic effects of quercetin in streptozotocin-induced diabetic rats. Toxicol Pharmacol 135: 357-364,2003
  5. Song HS. The Encyclopedia of Crops in Korea. Pulkotnamoo, 1998
  6. Ahn DK. The Herb lllustrated-book of Korea. Kyohaksa, 1998
  7. Grover JK, Adiga G, Vats V, Rathi S. Extracts of Benincasa hispida prevent development of experimental ulcers. J Ethnophannacol 78: 159-164,2001 https://doi.org/10.1016/S0378-8741(01)00334-8
  8. Grover JK, Adiga G, Vats V, Rathi S. Preliminary study of fresh juice of Benincasa hispida on morphine addiction in mice. Fitoterapia 71: 707-709, 2000 https://doi.org/10.1016/S0367-326X(00)00227-6
  9. Dong HJ. The Handbook of Oriental Medicine. Namsandang, p. 1170,1994
  10. Yoshizumi S, Murakami T, Kadoya M, Matsuda H, Yamahara J, Yoshikawa M. Medicinal food stiffs. XI. Histamine release inhibitors from wax gourd, the fruits of Benincasa hispida cogn. Cogn Yakugaku Zasshi 118: 188-192, 1998
  11. Uchikoba T, Yonezawa K, Kaneda M. Cucurnisin like protease from the sarcocarp of Benincasa hispida var. Ryukyu Phytochem 49: 2215-2219, 1998 https://doi.org/10.1016/S0031-9422(98)00135-6
  12. Lee KS, Ahn DK, Shin MK, Kim CM. Encyclopedia of Chinese Medicine, pp.1392-1399. Jeong Dam, Seoul, 1997
  13. National products research institute. Seoul national university. Treatise on Asian herbal medicines, 2003
  14. Lim SJ, Jeong JG, Kim MW, Choi SS, Han HK, Park JE. Effects of Benincasa hispida intake on blood glucose and lipid levels in streptozotocin induced diabetic rats. Korean J Nutr 36: 335-343, 2003
  15. Lim SJ, Kim YR. Effects of Benincasc hispida seeds intake on blood glucose and lipid levels in streptozotocin induced diabetic rats. Korean J Nutr 37: 266-272, 2004
  16. Reeves PG. Components of the AIN-93 diets as improvements in the AIN-76A diet. J Nutr 127: 838S-841S, 1997
  17. Rakieten N, Rakieten ML, Nadkami MV. Studies on the diabetogenic actions of streptozotocin. Cancer Chemather Rep 29: 91-98, 1963
  18. Junod A, Lambert AE, Stauffacher W, Renod AE. Diabetogenic action of streptozotocin: relationship of dose to metabolic response. J Clin Invest 48: 2129-2139, 1969 https://doi.org/10.1172/JCI106180
  19. Junod A, Lambert AE, Orci L. Picet R, Gonet AE, Renold AE. Studies of the diabetogenic action of streptozotocin. Proc Soc Exp BioI Med 126: 201-205, 1967
  20. Samson M. Fehlmann M, Dolais-Kitabgi J, Freychet P. Amino acid transport in isolated hepatocytes from streptozotocin diabetic rats. Diabetes 29: 996-1000, 1980 https://doi.org/10.2337/diabetes.29.12.996
  21. Wilson GL. Mechanism of streptozotocin-induced and alloxaninduced damage in rat $\beta$-cells. Diabetologia 27: 587-591, 1984 https://doi.org/10.1007/BF00276973
  22. Hassid WZ, Abraham X. Chemical procedures for analysis of polysaccharides In: methods in enzymology 3. Academic press. pp.34-50, 1957
  23. Giegel JL, Ham SB, Clema W. Serum triglyceride determined colorimetry with an enzyme that produces hydrogen peroxide. J Clin Chem 24: 931-933, 1978
  24. Lowry OH, Rosebrough NJ, Farr AJ, and Randall RR. Protein measurement with the foline phenol reagent. J BioI Chem 193: 265-273, 1951
  25. Uchiyama M, Mihara M. Determination of malondialdehyde precursor in tissue by thiobarbituric acid test. Anal Biochem 86: 271-278,1978 https://doi.org/10.1016/0003-2697(78)90342-1
  26. Meglasson MD, Burch PT, Berner DK, Najafi H, Matschinsky FM. Identification of glucokinase as an alloxan-sensitive glucose sensor of the pancreatic $\beta$-eells. Diabetes 35: 1163-1173, 1986 https://doi.org/10.2337/diabetes.35.10.1163
  27. Rhee SJ, Choe WK, Cha BK, Yang JA, Kim KY. Effects of vitamin E and selenium on the antioxidative defense system in streptozotocin-induced diabetic rats. Korean J Nutr 29: 22-31, 1996
  28. Lombardo YB, Serdikoff C, Thamotharan M, Paul HS, Adibi S. Inverse alterations of BCKA dehydrogenase activity in cardiac and skeletal muscles of diabetic rats. Am J Physiol 40: E685-E692, 1999
  29. Thompson-Culin K. Zussman B. Miller AK. Freed MI. Pharmacokinetics of rosiglitazone in patients with end-stage renal disease. J Inter Med Res 30: 391-399,2002 https://doi.org/10.1177/147323000203000405
  30. Liu SM, Barac-Nieto M. Renal protein degradation in streptozotocin diabetic mice. Diabetes Res Clin Pract 34: 143-148, 1997 https://doi.org/10.1016/S0168-8227(96)01353-8
  31. Ng TB, Parkash A, Tso SS. Purification and characterization of $\alpha$- and $\beta$-benincasins, arginine/glutamate-rich peptides with translation-inhibiting activity from wax gourd seeds. Peptides 24: 11-16,2003 https://doi.org/10.1016/S0196-9781(02)00271-1
  32. Kim HK, Cho DW, Hahm YT. The effects of Coix bran on lipid metabolism and glucose challenge in hyperlipidemic and diabetic rats. J Korean Soc Food Sci Nutr 29: 140-146,2000
  33. Rosenson RS, Shott S, Tangney CC. Hypertriglyceridemia is associated with an elevated blood viscosity rosenson: triglycerides and blood viscosity. Atherosclerosis 161: 433-439, 2002 https://doi.org/10.1016/S0021-9150(01)00656-6
  34. Listenberger LL, Han X, Lewis SE, Cases S, Farese Jr RV, Ory DS, Schaffer JE. Triglyceride accumulation protects against fatty acid-induced lipotoxicity. PNAS 100: 3077-3082,2003 https://doi.org/10.1073/pnas.0630588100
  35. Sachdewa A, Khemani LD. Effect of Hibiscus rosa sinensis Linn. Ethanol flower extract on blood glucose and lipid profile in streptozotocin induced diabetes in rats. J Ethnopharmacol 89: 61-66, 2004 https://doi.org/10.1016/S0378-8741(03)00230-7
  36. Jennings PE, Bamett AH. New approaches to the pathogenesis and treatment of diabetic microangiopathy. Diabetes Med 5: 111-117,1988 https://doi.org/10.1111/j.1464-5491.1988.tb00955.x
  37. Yue-Kevin KM, Chung WS, Leung Alvert WN, Christopher H, Cheng K. Redox changes precede the occurrence of oxidative stress in eyes and aorta, but not in kidneys of diabetic rats. Life Sci 73: 2557-2570, 2003 https://doi.org/10.1016/S0024-3205(03)00662-3
  38. Unlucerci Y, Kocak H, Seferoilu G, Bekpinar S. The effect of aminoguanidine on diabetes-induced inactivation of kidney Na+, K+-Atpase in rats. Pharmacological Res 44: 95-98, 2001 https://doi.org/10.1006/phrs.2001.0842
  39. Jang YY, Song JH, Shin YK, Han ES, Lee CS. Protective effect of boldine on oxidative mitochondrial damage in streptozotocininduced diabetic rats. Pharmacological Res 42: 361-371,2000 https://doi.org/10.1006/phrs.2000.0705
  40. Gupta S, Kataria M, Gupta PK, Murganandan S, Yashroy RC. Protective role of extracts of neem seeds in diabetes caused by streptozotocin in rats. J Ethnopharmacol 90: 185-189,2004 https://doi.org/10.1016/j.jep.2003.09.024
  41. Pushparaj P, Tan CH, Tan BKH. Effects of Averrhoa bilimbi leaf extract on blood glucose and lipids in streptozotocin-diabetic rats. J Ethnopharmacol 72: 69-76,2000 https://doi.org/10.1016/S0378-8741(00)00200-2
  42. Vats V, Yadav SP, Grover JK. Ethanolic extract of Ocimum sanctum leaves partially attenuates streptozotocin-induced alterations in glycogen content and carbohydrate metabolism in rats. J Ethnopharmacol 90: 155-160,2004 https://doi.org/10.1016/j.jep.2003.09.034
  43. Ahmed M, Lakhani S, Gillet M, John A, Raza H. Hypotriglyceridemic and hypocholesterolemic effects of anti-diabetic Momardica charantia (karela) fruit extract in streptozotocin-induced diabetic rats. Diabetes Res Clin Pract 51: 155-161, 2001 https://doi.org/10.1016/S0168-8227(00)00224-2