DOI QR코드

DOI QR Code

Optimum Lens Organization and System Set-up for 2-Dimensional Imaging of Biophoton

생물 광자의 이차원 영상을 위한 렌즈계 최적화와 장치 구성

  • Yi Seung-Ho (Dept. of Meridian & Acupuncture, College of Korean Medicine, Kyung Hee University) ;
  • Shim Seong-Bo (Optics Lab, School of Physics, Seoul National University) ;
  • Kim Jai-Soon (Optics Lab, School of Physics, Seoul National University) ;
  • Yang Joon-Mo (Biomedical Lab, School of Physics, Seoul National University) ;
  • Lee Changhoon (Biomedical Lab, School of Physics, Seoul National University) ;
  • Soh Kwang-Sup (Biomedical Lab, School of Physics, Seoul National University)
  • 이승호 (경희대학교, 한의대 경혈학교실) ;
  • 심성보 (서울대학교 자연과학대학 물리학부 광학 연구실) ;
  • 김재순 (서울대학교 자연과학대학 물리학부 광학 연구실) ;
  • 양준모 (서울대학교 자연과학대학 물리학부 한의학 물리 연구실) ;
  • 이창훈 (서울대학교 자연과학대학 물리학부 한의학 물리 연구실) ;
  • 소광섭 (서울대학교 자연과학대학 물리학부 한의학 물리 연구실)
  • Published : 2004.12.01

Abstract

We developed a 2-D imaging system to detect ultra weak biophoton by using a high sensitive fast CCD camera. We installed an illumination apparatus to generate delayed luminescence from plant leaves. Shutters at the illumination system and detector were synchronized to each other. The lens system of the CCD camera was newly designed to accommodate the specific requirements to enhance the collection efficiency for biophoton. Based on the lens simulation, commercial lenses were chose for the lens system. All the equipment was installed in a multi-purpose dark box and the delayed luminescence of Euonymus japonica was successfully acquired.

초 미약 생물광자의 이차원적 영상을 얻기 위해 고속 초고감도 CCD 사진기를 검출기로 사용한 실험 장치를 고안하였다. 본 장치에는 식물의 잎으로부터 방출하는 지연광 (delayed luminescence)를 발생시키기 위한 조명 장치를 설치하였으며, 조명 장치와 검출기에 있는 셔터들은 서로 연동되어 동작하도록 이루어졌다. CCD 사진기에 사용된 렌즈계는, 상용렌즈들로만 구성되었는데, 시료에서 나오는 생물광자의 집광 효율 (collection efficiency)을 높이도록 최적화된 렌즈 설계에서 나온 결과를 기초로 하였다. 이 장치들을 다용도 암 상자에 장착하여 사철나무 잎에서 나오는 지연광의 영상을 성공적으로 얻었다.

Keywords

References

  1. A. G. Gurvich, 'Ueber den Begriff des embryonalen Feldes,' Arch. Entw. Mech. Org., vol. 51, pp. 383-415, 1922
  2. F. A. Popp, et. al. 'Biophoton emission, new evidence for coherence and DNA as source,' Cell Biophysics, vol. 6, pp. 33-52, 1984 https://doi.org/10.1007/BF02788579
  3. M. Kobayashi, et. al. 'Two-dimensional photon counting imaging and spatiotemporal characterization of ultraweak photon emission from a rat's brain in vivo,' J of Neuroscience Methods, vol. 93, pp. 163-168, 1999 https://doi.org/10.1016/S0165-0270(99)00140-5
  4. F. A. Popp, J. J. Chang, A. Herzog, Z. Yan and Y. Yan, 'Evidence of non-classical (squeezed) light in biological systems,' vol. 293, no. 1-2, pp. 98-102, 2002 https://doi.org/10.1016/S0375-9601(01)00832-5
  5. F.-A. Popp, K. H. Li, W. P. Mei, M. Galle and R. Neurohr, 'Physical aspects of biophotons,' Experimentia, vol. 44, pp. 576-585, 1988 https://doi.org/10.1007/BF01953305
  6. M. Kobayashi, B. Devaraj, M. Usa, Y. Tanno, M. Takeda and H. lnaba, 'Two-dimensional imaging of ultraweak photon emission from germinating soybean seedlings with a high sensitive CCD camera,' Photochemistry and Photobiology, vol. 65 no. 3, pp. 535-537. 1997 https://doi.org/10.1111/j.1751-1097.1997.tb08600.x
  7. V. Vladimir, 'Reactive oxygen species, water, photons, and life,' Rivista di Biologia/Biology Forum., vol. 94, no. 2 pp. 237-258. 2001
  8. F. Musumeci, A. Triglia, and F. Grasso, 'Experimental evidence on Ultraweak photon emission from normal and tumour human tissues,' in Recent Advances in Biophoton Research (F. A. Popp et. al ed. World Scientific, Singapore, 1992) pp. 307-326
  9. M. Takeda, Y. Tanno, M. Kobayashi, M. Usa, N. Ohuchi, S. Satomi and H. Inaba, 'A novel method of assessing carinaoma cell proliferation by biophoton emission,' Cancer Lett., vol. 127, pp. 155-160, 1998 https://doi.org/10.1016/S0304-3835(98)00064-0
  10. B. Devaraj, M.Usa and H. Inaba, 'Biophotons: ultraweak light emission from living systems,' Current Opinion in Solid State & Materials Science, vol. 2, pp. 188-193, 1997 https://doi.org/10.1016/S1359-0286(97)80064-2
  11. ZEMAX, Optical Design Program, User's Guide, Ver10, Focus software, Incorporated: July, 2002
  12. Francis A. Jenkins, Harvey E. White, Fundamentals of Optics, 4th edition, pp.150. 1976
  13. Warren J. Smith, Modem Optical Engineering, 3rd edition, 2001
  14. Rudolf Kingslake, Lens Design Fundamentals, 1978