Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Association of Genetic Missense Mutation and Economic Traits of Leptin Gene using PCR-RFLP in Korea C밟le(Han-Wo이

PCR-RFLP를 이용한 한우 Leptin gene의 유전자형 변이와 경제형질과의 관련성 분석

  • Lim, H.Y. (Genomic Informatics Center, Hankyong National University) ;
  • Oh, J.D. (Genomic Informatics Center, Hankyong National University) ;
  • Kong, H.S. (Genomic Informatics Center, Hankyong National University) ;
  • Jeon, G.J (Genomic Informatics Center, Hankyong National University) ;
  • Lee, H.K. (Genomic Informatics Center, Hankyong National University) ;
  • Lee, S.S. (Genomic Informatics Center, Hankyong National University) ;
  • Yoon, D.H. (National Livestock Research Institute) ;
  • Kim, C.D. (National Livestock Research Institute) ;
  • Cho, B.W. (Dept. of Animal Science, Miryang National University)
  • 임현진 (국립한경대학교 유전정보연구소) ;
  • 오재돈 (국립한경대학교 유전정보연구소) ;
  • 공홍식 (국립한경대학교 유전정보연구소) ;
  • 전광주 (국립한경대학교 유전정보연구소) ;
  • 이학교 (국립한경대학교 유전정보연구소) ;
  • 이승수 (국립한경대학교 유전정보연구소) ;
  • 윤두학 (축산연구소) ;
  • 김종대 (축산연구소) ;
  • 조병욱 (밀양대학교)
  • Published : 2004.06.30
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Abstract

The identification of the leptin gene in 1994 and it's adipocytes specific protein leptin hal provided the first physiological links to the regulatory system controlling body weight and fat deposits. The meat tastes is mainly determined by quantifY and quality of triglyceride stored in adipose tissue. This study was conducted to analyze genetic cbaracteristics of Hanwoo leptin gene and also to investigate the association of DNA marlcer with some economic meat traits for Hanwoo. The leptin hormone gene polymorphisms were identified by digestion with Kpn2 I and Msp I. Slaughter weight(SWI), slaughter peroentage(SP), longissimus muscle area(LMA), beef marbling score(MS) and back fat thickness(BF) were compared among three genotypes by P(R..RFlJ> and showed significant differences among genotypes. PCR-RFLP(Kpn2 I) were detected significant for SP, MS and BF. The allele was essociated with fatter carcasses and C allele with leaner carcasses.

본 연구는 도체성적을 보유하고 있는 제 31차, 32차 한우 후보종모우 집단 228두를 선발하여 DNA를 분리 정제 후 exon 2에 위치한 bovine leptin gene 염기서열 가운데 특정 염기서열을 갖는 2좌위의 primer를 합성하여 PCR 수행 후 PCR-product를 이용하여 2 종류의 제한효소 Kpn 2 I, Msp I 으로 반응시킨 후 두 가지 형태의 대립 유전자를 검정하여 경제형질과의 관련성을 분석하였다. PCR-RFLP를 통하여 얻어진 leptin gene의 유전자형 빈도는 Kpn2 I의 경우 C 유전자 빈도(0.25)보다 T 유전자 빈도(0.75)가 높게 나타났으며 Msp I으로 처리한 경우 M 유전자 빈도(0.35)보다 m 유전자 빈도(0.65)가 높게 나타났다. 통계적 분석을 통하여 각 유전자형에 대한 경제형질과의 관련성을 분석한 결과 제한효소 Kpn2 I으로 처리한 경우 도체율에서 CT 유전자형과 CC 유전자형 사이에 유의적 차이가 나타났으며(P < 0.05), Msp I의 경우 도체중 Mm 유전자형과 mm 유전자형 사이에 통계적 유의성이 나타났다(P < 0.05).

Keywords

References

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