Mycobiology

The Korean Society of Mycology (한국균학회)
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A New Technique for Single Spore Isolation of Two Predacious Fungi Forming Constricting Ring

  • Singh, Kalika Prasad (Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University) ;
  • Kumar, Dharmendra (Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University) ;
  • Bandyopadhyay, Pinaki (Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University)
  • Published : 2004.12.31
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Abstract

A new technique for single spore isolation was developed for predacious fungi forming constricting rings directly on the spores using Dactylaria brochopaga and Arthrobotrys dactyloides. Constricting rings were induced directly on the spores by transferring the spores in 25 ppm solution of DL-Valine in sterile distilled water. Freshly hatched and thoroughly washed second stage juveniles of Meloidogyne incognita were transferred into cavity blocks containing induced rings for trapping and killing of nematodes. The killed nematodes were surface sterilized with streptomycin and inoculated into petri dishes containing maize meal agar media with 100 ppm streptomycin. The petri dishes were incubated at $29{\pm}1^{\circ}C$ for few days which yielded axenic culture of these fungi.

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References

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  5. from China vol.55, pp.8, 2015, https://doi.org/10.1002/jobm.201400909
  6. Single Spore Isolation as a Simple and Efficient Technique to obtain fungal pure culture vol.140, pp.1755-1315, 2018, https://doi.org/10.1088/1755-1315/140/1/012055
  7. Nematophagous Fungi Associated with Root Galls of Rice Caused by Meloidogyne graminicola and its Control by Arthrobotrys dactyloides and Dactylaria brochopaga vol.155, pp.4, 2007, https://doi.org/10.1111/j.1439-0434.2007.01208.x