Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Inhibitory Effect of Methanolic Extract from Adenophorae Radix on Melanogenesis

사삼 메탄올 추출물의 멜라닌생성 억제효과

  • Lim Nan Young (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kwon Gang Joo (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kim Youn Seok (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Baik Soon Ki (Department of Environmental EM, Dongshin University) ;
  • Lim Ju Rak (Jinan Medical Herbs Experimental Station, Jeonbuk Provincial ARES) ;
  • Mun Yeun Ja (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Woo Won Hong (Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 임난영 (원광대학교 한의학전문대학원) ;
  • 권강주 (원광대학교 한의학전문대학원) ;
  • 김윤석 (원광대학교 한의학전문대학원) ;
  • 백순기 (동신대학교 환경공학과) ;
  • 임주락 (전라북도농업기술원, 진안숙근약초시험장) ;
  • 문연자 (원광대학교 한의학전문대학원) ;
  • 우원홍 (원광대학교 한의학전문대학원)
  • Published : 2004.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Effects of methanolic extract from Adenophorae Radix (AR) on melanogenesis were investigated in mouse melanoma B16F10 cells. The methanol extract of AR was partitioned into Hexane, Ethyl acetate (EA), Butanol, H₂O, and exogenously added to the culture medium for 72 hours at the concentration of 10, 50, 100 and 200 ㎍/㎖. Of the four partitions, Hexane and EA partion of AR reduced tyrosinase activity, which is the key enzyme for a melanogenesis, as well as melanin contents. But the EA partition was less toxic for B16F10 cells and has more efficient melanin-reducing effect than the former. In addition, the EA partition dramatically lightened the color of cell pellet and significantly decreased the level of tyrosinase protein expression. In these results, EA partition of AR reduced melanin synthesis of B16F10 mouse melanoma cells by down regulating the tyrosinase activity and tyrosinase protein expression. Therefore, it is anticipated that AR is a candidate for an efficient whitening agent which supresses melanogenesis.

Keywords

References

  1. 조직학 박경아 등
  2. 한의학논문집 v.8 no.2 사삼 (沙渗)에 대한 문헌적 고찰 서영배;신동훈
  3. 한국의 보약 최태섭
  4. Hifu. Skin Res. v.34 Clinical effects of arbuein in patients with chloasma in Japanese. Sugai, T.
  5. Plast Reconstr. Surg. v.85 Experimental study on the hyperpigmentation of skin graft. Kim, C. W.
  6. J. Invest. Dermatol. v.81 Selective aberration and pigment loss in melanosomes of maligment melanoma cells in vitro by glycosylation inhibitors: Premelanosomes as glycoprotein. Mishima, Y.;Imokawa, G.
  7. Cancer Res. v.42 Loss of melanogenic properties in tyrosinase induced by glucosylation inhibitors within malignant melanoma cells. Imokawa, G.;Mishima, Y.
  8. J. Invest. Dermatol. Proc. v.4 Biochemical control of melanogenesis and melanosomal organization. Hearing, V. J.
  9. Arch Dermatol. Res. v.278 Differential analysis of experimental hypermelanosis induced by UVB, PUVA and allergic dermatitis using a brownish guinea pig model. Imokawa, G.;Kawai, M.;Mishima, Y.;Motegi, I.
  10. Pigment Cell Res. v.7 Analysis of the UV-induced melanogenesis and growth arrest of human melanocytes. Abdel-Malek, Z.; Swope, V. B.;Smalara, D.;Babcock, G.;Dawes, S.;Nordlund, J. J.
  11. Dermatovenerologija, Zagreb. Dobric. Grafoplast Skin infections caused by protozoa. Stipic, V.;Dobric, I.(ed.)
  12. EMBO j. v.13 Tyrosinase related protein (TRP-1)functions as a DHICA oxidase activity in melanin biosynthesis Kobayashi T;Urabe K;Winder Aj(Etal.)
  13. Biochemical Pharmacology v.57 Inhibitors of mamalian melanocytes tyrosinase: In vitro comparisons of alkyl esters of gentisic acid with other putative inhibitors. Curto E V;Kwong C;Hermersdorfer H;Glatt H;Santis C;Virador V;Hearing V J;Dooley P
  14. Life Science. v.65 no.21 Inhibition of tyrosinase by green tea components Jk NO;DY Soung;YJ Kim;KH Shim;YS Jun;SH Rhee;T Yokizawa;HY Chung
  15. J. Invest. Dermatol. v.75 no.122 Recent advances in the chemistry of melanogenesis in mammals. Prota G.
  16. Yakhak Hoeji. v.42 no.4 Isolation of inhibitory components oa tyrosinase activity from the bark of Paeonia moutan. SH Lee;JS Park;SY kim;JJ kim;SR Chung
  17. A textbook of histology Bloom W;Fawcett DW
  18. J. Photochemistry and Photobiology B. Biology v.61 Melanin content of cultured human melanocytes and UV-induced cytoyoxicity. J. Photochemistry and Photobiology Sandra M. De Leeuw;Nico P. M. Smit;Monique Van Veldhoven;Ed M. Pennings;Stan Pavel;Johannes W. I. M. Simons;Albert A. Schothorst
  19. Hifu. Skin Res. v.34 Clinical effects of arbutin in patients with chlosma in Japanease. Sugai, T
  20. Biochemical Pharmacology. v.57 Inhibitors of mamalian melanocytes tyrosinase: In vitro comparisons of alkyl esters of gentisic acid with other putative inhibitors. Curto E V;Kwong C;Hermersdorfer H;Glatt H;Santis C;Virador V;Hearing V J;Dooley P
  21. 본초학회지 v.7 no.1 제니와 사삼중의 유리당 함량 및 효능에 관한 비교연구 안덕균;김재학
  22. v.15 no.1 사삼의 면역조절 작용에 대한 실험적 연구 신동훈;서영배
  23. 동의생리병리학회지 v.18 no.2 沙參 물 추출물의 멜라닌 형성 억제 효과 강현성;임난영;문연자;임홍진;박민철;임규상;김남권;우원홍
  24. J. Immun. Methods. v.65 Rapid colorimetric assay for the cellular growth and survival; application to proliferation and cytotoxic assay. Mosmann, T.
  25. Eur. J, Biochem. v.255 Mechanism of melanogenesis inhibition by tumor necrosis factor­alpha in B16/F10 mouse melanoma cells. Martinez­Esparza, M.;Jimenez-Cervantes, C.;Solano, F.;Lozano, J.A.;Garcia­Borron, J. C.
  26. Cancer Res v.45 Regulation of melanin synthesis of B16 mouse melanoma cells by 1$\alpha$,25­dehydroxyvitamin D3 and retinoic acid. Hosei, J.;E.;Suda, T.;Kuroki, T.
  27. Original Res Does $\alpha$-MSH have a role in regulating skin pigmentation in humans. Anthony J. Thody;Alison Graham
  28. 임상본초학 신민교
  29. 中藥大辭典 이경순(외)
  30. 한국 본초도감 안덕균
  31. 동의생리병리학회지 v.16 no.6 감초 물추출물의 멜라닌 형성 억제효과 문연자;김진;임난영;이승연;곽섭;황충연;우원홍
  32. Exp Cell Res v.207 no.1 Eumelanin biosynthesis is regulated by coordinate expression of tyrosinase and tyrosinase-related protein-1genes. Kuzumaki T;Matsuda A;Wakamatsu K;Ito S;Ishikawa K
  33. J Invest Dermatol v.107 no.5 Regulation of tyrosinase mRNA in mouse melanoma cells by alpha-melanocyte- stimulating hormone. Rungta D;Corn TD;Fuller BB
  34. Pigment Cell Res v.1 Induction of melanogenesis suppression: Cellular pharmacology and mode of differential action. Mishima Y.;Hatta S.;Ohyma, Y.;Inazu, M.
  35. Pigment Cell Res v.10 Biological role of tyrosinase related protein and its biosynthesis and transport from TGN to stage I melanosome, late endosome, througt gene transfection study. Jimbow K;Gomez PF;Toyofuku K;Chang D;Miura S;Tsujiya H;Park JS
  36. 대전대학교 한의학연구소 논문집 v.8 no.2 沙蔘에 관한 문헌적 고찰 신동훈(외)
  37. 대한한의학 방제학회지 v.10 no.2 더덕추출물이 멜라닌생성에 미치는 영향 이승연;김진만;오현철;임숙정;황충연;문연자;우원홍
  38. 약학회지 v.46 no.2 소목의 부탄올 추출물이 B16/F10 흑색종세포의 멜라닌 합성에 미치는 효과 천현자;황상구;정동훈;백승화;전병훈;우원홍
  39. 동의생리병리학회지 v.15 no.6 소목의 에틸아세테이트 추출물이 B169. 천현자, 김용수, 남항/F10 흑색종세포의 멜라닌 합성에 미치는 효과 천현자;김용수;남항우;윤성찬;우원홍