Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Spermiogenesis in the Korean Squirrel, Tamias sibiricus

다람쥐(Tamias sibiricus)의 정자변태

  • Jung, Tae-Dong (Division of Biology and chemistry, College of Natural Scineces, Kyungnam University) ;
  • Lee, Jung-Hun (Division of Biology and chemistry, College of Natural Scineces, Kyungnam University) ;
  • Kim, Sang-Sik (Electron Microscopy Facility, Department of Ophthalmology, Pusan National University Hospital)
  • 정태동 (경남대학교 자연과학대학 생명화학부) ;
  • 이정훈 (경남대학교 자연과학대학 생명화학부) ;
  • 김상식 (부산대학교 대학병원)
  • Published : 2004.09.01
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Abstract

Spermiogenesis in the Korean squirrel, Tamias sibiricus, was investigated by transmission electron microscopy. Spermiogenesis was divided into Golgi, cap, acrosome, maturation and spermiation phases based on the characteristics of acrosomal changes and nuclear shape. Beside, the Golgi, cap and acrosomal steps were subdivided into three phases of early, middle and late phase respectively, the maturation step was divided into two phases of early and late phase, and spermiation step has only one phase. Thus, the spermiognesis of T. sibiricus was divided into a total of twelve phases. In Golgi phase (steps 1-3), a well developed Golgi complex was located close to the vesicles, the acrosomal vesicle fixd to a recess of nuclear membrane at step 3. During cap phase (steps 4-6), the acrosomal vesicle spred over the nuclear surface to cover a third of the nucleus, and the acrosomal granule was not yet flattened. At acrosomal phase (steps 7-9), the nucleus and acrosome were elongated but nucleoplasm was not condensed. During maturation phase (steps 10-11), the nucleoplasm was more condensed, and the mitochondria completely arranged the center of axoneme. The spatulate-sperm head was completely formed at spermiation phase (step 12).

한국산 다람쥐(Tamias sibiricus)의 정자변태 과정을 투과전자현미경으로 조사하였다. 정자변태는 첨체변화와 핵의 형태의 특징을 기초로 하여 골지기, 두모기, 첨체기, 성숙기 그리고 이탈기로 구분하였고, 골지 두모 첨체기는 각각 전 중 후기, 성숙기는 전 후기, 이탈기는 1기로 세분하였다. 따라서 다람쥐(Tamias sibiricus)의 정자변태는 12기(phases)로 구분되어졌다. 골지기(steps 1-3)의 경우, 잘 발달된 골지복합체는 첨체소포가까이에 위치하고, 첨체소포는 3단계에서 핵막과 융합하여 함입되어있다. 두모기(steps 4-6)에서는, 첨체소포가 핵의 표면 위에 넓게 퍼지며 핵의 1/3을 덮고, 첨체과립은 아직 분산되지 않았다. 첨체기(steps 7-9)동안에 있어서, 핵과 첨체는 신장되었으나 핵질은 농축되지 않았다. 성숙기(steps 10-11)에서는 핵질이 더욱 농축되어 졌으며, 미토콘드리아들은 축사의 중심에 완전하게 배열되어졌다. 이탈기(step 12)에서 정자머리는 완전하게 주걱형태의 모양을 갖추고 있었다.

Keywords

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