Korean Journal of Plant Taxonomy (식물분류학회지)

The Korean Society of Plant Taxonomists (한국식물분류학회)
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RAPD marker variations between and within the species of Korean Suaeda

한국산 나문재속의 종내·종간 RAPD marker 변이

  • 심현보 (인하대학교 이과대학 생명과학과) ;
  • 최병희 (인하대학교 이과대학 생명과학과)
  • Received : 2004.03.09
  • Accepted : 2004.03.17
  • Published : 2004.03.31
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Abstract

The genus Suaeda is one of the most popular plants on salt marsh areas in Korean Peninsular. The entities that comprise taxa in Korea exhibit widely overlapping ranges in all morphological attributes. Ramdom amplified polimorphic DNA (RAPD) markers were used to clarify taxonomic delimitation among the Korean taxa of Suaeda and to analyse genetic variations among the populations of S. japoniro in western and southern coastal regions. Six decamer primers amplified a total of 65 scorable bands, of which 61 were polymorphic. In all primers investigated, S. glauro of sect. Schanginia is the most distinctive species, compared with others of sect. Heterosperma. S. japonica, S. maritima, and S. malacosperma, which have been hardly distinguished each other by external morphology, were readily recognized by its specific DNA bands. The characteristic RAPD markers were identified local populations of S. japonica, but this feature was not revealed within population.

나문재속은 염습지에서 가장 흔히 자라는 식물인데, 한국산 나문재속은 형태적 변이가 심하여 종 식별이 어렵다. 본 연구는 RAPD분석을 통해 한국산 나문재속의 종간 분류학적 한계를 명확히 하고, 서, 남해안에서 칠면초 집단간의 유전적 변이를 알아보고자 수행되었다. 실험에 사용된 6개의 primer로부터 65개의 유용한 band를 얻었는데, 그 중 61개가 다형성이었다. RAPD 분석결과 Schanginia절에 속하는 나문재는 Heterosperma절에 속하는 나머지 종들과 조사된 모든 primer에서 뚜렷한 차이를 보였다. 또한 외부형태적으로 구별이 어려운 칠변초, 해홍나물, 기수초에서 종간에 차이가 있는 DNA band가 발견되었다. 칠면초 집단에 대한 조사에서 지역 집단간에 차이를 보이는 RAPD marker가 나타났으나, 지역 내 생육지에 따른 변이는 없었다.

Keywords

References

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