KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Characteristics of Cell Growth and Poly[3-hydroxybutyrate-co-4-hydroxybutyrate] Synthesis by Alcaligenes latus and Comamonas acidovorans

Alcaligenes latus와 Comamonas acidovorans의 균체성장 및 Poly[3-hydroxybutyrate-co-4-hydroxybutyrate] 합성 특성

  • Song Jae Yang (Department of Chemical Engineering, Chungbuk National University) ;
  • Kim Beam Soo (Department of Chemical Engineering, Chungbuk National University)
  • 송재용 (충북대학교 공과대학 화학공학과) ;
  • 김범수 (충북대학교 공과대학 화학공학과)
  • Published : 2004.10.01
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Abstract

Characteristics of cell growth and poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] synthesis was investigated through flask and batch cultures of Alcaligenes latus and Comamonas acidovorans. The specific growth rate of C. acidovorans increased with yeast extract concentration and decreased with 1,4-butanediol concentration. Optimum glucose concentration for growth of C. acidovorans was 20 g/L. In one-step flask cultures of C. acidovorans, final dry cell weight and PHA content decreased with the ratio of 1,4-butanediol to glucose, while the 4HB fraction in copolymers gradually increased to 100 $mol\%$ with an initial 1,4-butanediol concentration of 20 g/L as single carbon source. The specific growth rate of A. latus decreased with v-butyrolactone concentration and optimum sucrose concentration for growth was 10 g/L. In batch cultures of A. latus, 4HB fraction increased with initial v-butyrolactone concentration. P(3HB-co-4HB) with 19 $mol\%$ 4HB was obtained when the initial ratio of v-butyloractone (g/L) to sucrose (g/L) was 10 : 10.

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References

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