Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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A Simple Purification Procedure of Biologically Active Recombinant Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF) Secreted in Rice Cell Suspension Culture

  • Sharma Niti (Institute for Molecular Biology and Genetics, Basic Science Research Institute, Chonbuk National University) ;
  • Park Seung Moon (Institute for Molecular Biology and Genetics, Basic Science Research Institute, Chonbuk National University) ;
  • Kwon Tae Ho (Institute for Molecular Biology and Genetics, Basic Science Research Institute, Chonbuk National University) ;
  • Kim Dae Hyuk (Institute for Molecular Biology and Genetics, Basic Science Research Institute, Chonbuk National University) ;
  • Yang Moon Sik (Institute for Molecular Biology and Genetics, Basic Science Research Institute, Chonbuk National University)
  • Published : 2004.11.01
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Abstract

A simple purification procedure of bioactive human granulocyte macrophage colony stimulating factor (hGM-CSF) secreted in rice cell suspension culture has previously been described. In this study the protein was purified to apparent homogeneity with an overall yield of $80.1\%$ by ammonium sulfate precipitation and a single chromatographic step involving FPLCanion exchange chromatography. The purified hGM-CSF revealed at least five glycosylated forms ranging from $21.5{\~}29$ kDa, and its biological activity was independent of the glycosylation pattern. This is the first purification report of recombinant hGM-CSF to apparent homogeneity from rice cell suspension cultures.

Keywords

References

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