Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Physiology and Growth Properties of Halophilic Bacteria Isolated from Jeotgal (Salted Seafood)

젓갈(염장발효식품)에서 분리한 호염세균의 생리 및 성장특성

  • Jung Yoo Jeong (Department of Biological Engineering, Seokyeong University) ;
  • Park Doo Hyun (Department of Biological Engineering, Seokyeong University)
  • 정유정 (서경대학교 이공대학 생물공학과) ;
  • 박두현 (서경대학교 이공대학 생물공학과)
  • Published : 2004.12.01
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Abstract

Two species of halophilic bacteria were isolated from five salted seafoods and identified by 16S rDNA sequenc­ing homology. One was identified as Halomonas subglaciescola and other four strains were belong to Halomo­nas marina. The identity of all isolates with standard organisms was above $95\%.$ H. subglaciescola, H. marina IN, and H. marina SH-2 grew in salinity condition from $3%\;to\;18\%$ NaCl but growth of H. marina SQ and H. marina SH-l grew in salinity environment from $8\%\;to\;17\%.$ Maximum biomass of H. subglaciescola, H. marina IN, H. marina SQ, H. marina SH-1, and H. marina SH-2 growing in LB medium containing $15\%$ NaCl were about 3.2, 4.5, 4.5, 5.7, and 4.2, however the maximum biomass in LB medium containing $5\%$ NaCl were about 2.2, 1.1, 0.7, 0.2, and 2.4 as optical density at 660 nm, respectively. In scanning electron micrograph, unknown material (mucus) attached to outer membrane of all isolates was observed. When mucus isolated from halophilic bacterial cell was added to culture of E. coli, E. coli grew in medium containing $15\%$ NaCl.

다섯 종류의 젓갈로부터 다섯 종의 호염성 세균을 분리하였다. 이들의 165-rDNA 염기서열을 분석한 결과 한 종은 Halomonas subglaciescola, 다른 종들은 H. marina에 속하는 것으로 판명되었다. H. subglaciescola, H. marina IN, and H. marina SH-2는 염도가 $3\%$에서 $20\%$에 이르는 넓은 범위의 배양 조건에서, H. marina SQ and H. marina SH-1는 염도가 $8\%$ $19\%$에 이르는 비교적 좁은 범위의 배양 조건에서 성장하는 것으로 나타났다. $15\%$ NaCl을 포함하는 LB 배지에서 배양한 H. subglaciescola, H. marina IN, H. marina SQ, H. marina SH-1, H. marina SH-2의 최대 균체량은 흡광도로서 각각 3.2, 4.5, 4.5, 5.7, 4.2 이었으나 $5\%$의 NaCl을 포함하는 동일 배지에서 최대 균체량은 각각 2.2, 1.1, 0.7, 0.2, 2.4 이었다. 균체의 주사전자현미경 사진에서 균체의 표면에 부정형의 이물질이 부착되 어 있는 것으로 확인되었다. 각 균체의 배양액에서 점성을 갖는 물질을 분리하여 대장균의 배양액에 첨가하였을 때 대장균은 $15\%$ NaCl을 갖는 배지에서 성장하였다. 이것은 호염균의 세포 표면에 부착한 물질이 NaCl의 차단효과를 갖기 때문으로 생각된다.

Keywords

References

  1. 조덕제, 김우흥, 채수규, 홍종만, 1997, 식품분석. 지구문화사, 51-53
  2. Aharon, O. 1999. Bioenergetics aspects of halophilism. Microbiol. Mol. Biol. Rev. 63, 334-348
  3. Brison, J., D. Courtois, and F. Denis. 1974. Microbiological study of a hypersaline lake in French Somaliland. Appl. Microbiol. 27, 819-822
  4. Cánovas, D., C. Vargas, L., N. Csonka, A.Ventosa, and J.J.Nieto. 1996. Osmoprotectants in Halomonas elongata: high-affinity betaine transport system and choline-betaine pathway. J. Bacteriol. 178: 7221-7226
  5. Cánovas, D., C. Vargas, L.N. Sconka, A. Ventosa, and J.J. Nieto. 1998. Synthesis of glycine betaine from exogenous choline in the moderately halophilic bacterium Halomonas elongata. Appl. Environ. Microbiol. 64: 4095-4097
  6. Choquet, C.G., I. Ahoshai, M. Klein, and D.J. Kushner. 1991. Formation and role of glycine betaine in the moderate halophile Vibrio costicola, site for action of Cl$^-$ ions. J. Bacteriol. 171: 880- 886
  7. Hirsch, P. 1980. Distribution and pure culture studieds of morphologically distinct solar lake microorganism, P. 41-60. In A. Nissenbaum (ed.), Hypersaline brines and evaporitic environments. Elsevier/North-Holland Biomedical Press. Amstrerdam, The Nertherland
  8. Hirsch, P. and B. Hoffmann. 1989. Dichotomicrobium thermohalophilum, gen. Nov., spec. nov., budding prosthecate bacteria from the solar lake and some related strains. Syst. Appl. Microbiol. 11: 291-301
  9. Kushner, D.J. 1991. Growth and nutrition of halophilic bacteria, P.87-103. In R.H. Vreeland and L.I. Hochstein (ed.), The biology of halophilic bacteria. CRC Press, Inc., New York
  10. LeFevere, E. and L.A. Round. 1919. A preliminary report upon some halophilc bacteria. J. Bacteriol. 4: 177-182
  11. Lowt, S.E., M.K. Jain, and J.G. Zeikus. 1993. Biology, ecology, and biotechnological application of anaerobic bacteria adjusted to environmental stresses in temperature, pH, salinity, or substrates. Microbiol. Rev. 57: 451-509
  12. Martin, D.D., R.A. Ciulla, and M.F. Robert. 1999. Osmoadaptation in Archaea, Appl. Envir. Microbiol. 65: 1815-1825
  13. Marquez, M.C., A. Ventosa, and F. Ruiz-Berraquero. 1987. A taxonomic study of heterotrophic halophilic and non-halophilic bacteria from a solar saltern. Gen. Microbiol. 133: 202-208
  14. Miller, K.J. and S. Leschine. 1984. A halotolerant Planococcus from Antarctic dry valleys. Curr. Microbiol. 11: 205-210
  15. Mojica, F.J., E. Cisneros, C. Ferrer, F.R. Valera, and G. Juez. 1997. Osmotically induced response in representatives of halophilic prokaryotes, the bacterium Halomonas elongata and the archaeon Haloferax volcanii. J. Bacteriol. 179: 5471-5481
  16. Mullakhaubhai, M.F. and H. Larsen. 1975. Halobacterium volcanii spec. nov., a Dead Sea halobacterium with a moderate salt requirement. Arch. Microbiol. 104: 1231-1235
  17. Ollivire, B., P. Caumette, J.-L.Garcia, and R.A. Mah. 1994. Anaerobic bacteria from hypersaline environments. Microbiol. Rev. 58: 27-38
  18. Ryu, H.J., Y.J.Jeong, D.H.Park. 2004. Growth and physiological properties of wild type and mutants of Halomonas subglaciescola DH-1 in saline environment. J. Microbiol. 42: 174-180
  19. Skerman, V.B.D., V. McGowan, and P.H.A. Sneath (ed.). 1980. Approved list of bacterial names. Int. J. Syst. Bacteriol. 30: 225- 420
  20. Vreeland, R.H., C.D. Lithchfield, E.I. Martin, and E. Elliot. 1980. Halomonas elongata, a new genus and species of extremely salttolerant bacteria. Int. J. Syst. Bacteriol. 30: 485-495
  21. Vreeland, R.H., B.D. Mierau, C.D. Litchfield, and E.L. Martin. 1983. relationship of the internal solute composition to the salt tolerance of Halomonas elongata. Can. J. Microbiol. 29: 407-414
  22. Vreeland, R.H. 1987. Mechanisms of halotolerance in microorganisms, Crit. Rev. Microbiol. 143: 311-356