Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Studies on Biological Activities of the Polysaccharides and Oligosaccharides of Orostachys japonicus

와송 다당체 및 올리고당류의 생리 활성

  • Kim Ki Hoon (Department of Biomedical Laboratory Science and Biohealth Products Research Center, Inje University) ;
  • Kim Eun Young (Department of Biomedical Laboratory Science and Biohealth Products Research Center, Inje University) ;
  • Kim Yea Oon (Department of Biomedical Laboratory Science and Biohealth Products Research Center, Inje University) ;
  • Baek Geum Ok (Department of Biomedical Laboratory Science and Biohealth Products Research Center, Inje University) ;
  • Kim Han Bok (Department of Life Science, Hoseo University) ;
  • Lee Dong Seok (Department of Biomedical Laboratory Science and Biohealth Products Research Center, Inje University)
  • 김기훈 (인제대학교 의생명공학대학 임상병리학과, 인제대학교 바이오헬스 소재 연구센터) ;
  • 김은영 (인제대학교 의생명공학대학 임상병리학과, 인제대학교 바이오헬스 소재 연구센터) ;
  • 김예운 (인제대학교 의생명공학대학 임상병리학과, 인제대학교 바이오헬스 소재 연구센터) ;
  • 백금옥 (인제대학교 의생명공학대학 임상병리학과, 인제대학교 바이오헬스 소재 연구센터) ;
  • 김한복 (호서대학교 자연과학부 생명과학전공) ;
  • 이동석 (인제대학교 의생명공학대학 임상병리학과, 인제대학교 바이오헬스 소재 연구센터)
  • Published : 2004.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Polysaccharides were prepared from Orostachys japonicus by extration with hot steam water (OJPl). The OIPl fraction was further purified by Sephadex G-50 gel filtration chromatography to produce FI (polysaccharides) and FII (oligosaccharides) fraction. The average molecular masses o fFI and FII fraction were determined to be 3050 kDa and 13 kDa, respectively. The antimicrobial activity of OIPl was tested against 8 strains of bacteria and one strain of yeast by the disc diffusion method, fluorescein diacetate (FDA) method and broth dilution method. The OIPl exhibited a very strong growth inhibition to Candida albicans. The OIPl remarkably sup­pressed the growth of Salmonella typhimurium and Staphylococcus aureus. The OIPl showed higher growth inhibition to Escherichia coli and Pseudomonas aeruginosa than propolis, positive control. When the anticancer activity of the OIPl, FI or FII was examined against human cancer cell lines and the Sarcoma 180 cells, these widely suppressed the proliferation of cell lines in the MTT assay and morphology study. Especially, they remarkably inhibited the growth of A549, HeLa and AGS cells. Also treatment of cancer cells with OJPl, FI or FII induced apoptotic cell death characterized by DNA fragmentation. The OJPl, FI or FII exhibiting various biological activities such as antimicrobial activity and anticancer activity is expected to be developed as new biohealth products.

와송으로부터 고온 고압의 열수 추출로 다당체 조추출물(OJP1)을 얻어 디스크 확산법, fluorescein diacetate(PDA)법 및 액체 배지 희석법을 통하여 세균과 진균에 대한 항균 활성을 조사하였다. 또한 Sephadex G-50을 통하여 다당체(FI)와 올리고당류(FII)를 분리하고, 이들의 항암 활성을 조사하였다. FI과 FII의 분자량은 각각 30$\~$50 kDa과 1$\~$3 kDa으로 추정되었다. OJP1의 항균 효과는 디스크 확산법에서 Candida albicans가 $20\pm4.9\;mm$의 억제대로 가장 높았으며, Salmonella typhimurium과 Staphylococcus aureus도 각각 $18\pm2.0\;mm$ 와 $17\pm1.0\;mm$의 억제대로 비교적 높게 나타났다. 또한 Escherichia coli와 Pseudomonas aeruginosa에 대해서도 양성 대조군인 프로폴리스보다 높게 나타났으며, FDA법, 액체배지 희석법에서도 비슷한 양상을 나타냈다. OJP1과 Fl, FII의 인체 암세포주와 Sarcoma 180 세포주에 대한 항암 활성을 측정해 본 결과, 이들은 모두 MTT assay와 형태 변화 관찰에서 강력한 암세포주 증식 억제 효과를 나타내었는데, 특히 폐암 세포주인 A549 세포와 자궁 경부암 세포주인 HeLa 세포, 위암 세포주인 AGS에서 현저한 효과를 보였다. 더 나아가 DNA 분절화를 통해 apoptosis 유발 여부를 확인해 본 결과, 대조군에 비해서 이들 물질을 처리한 실험군에서 apoptosis발생을 뜻하는 DNA 분절화 현상이 뚜fut하게 나타났다. 요컨대, OJP1과 Fl, FII는 광범위한 병원성 균주에 대하여 효과적인 항균 활성을 보였으며, 각종 암세포주에 대하여 현저한 항암 활성을 나타내는 것으로 확인되었다.

Keywords

References

  1. 金在佶.1984.原色天然藥物 大事典(上卷). 447. 南山堂. 서울
  2. 小學館. 1985. 小學館編;中藥大事典. 526. 上悔科學技術. 出版社. 上悔
  3. 左春旭, 仲英, 姜岩靑. 1988. 瓦松 中黃美化合物的 分離鑑定 19, 148. 中草藥. 丁杏包
  4. 左春旭, 蔡玉英, 姜岩靑. 1985.瓦松有效成分的 硏究16, 243. 中草藥. 丁杏包
  5. Chakrabarty, S., M. Roy, B. Hazra and R.K. Bhattacharya. 2002. Induction of apoptosis in human cancer cell lines by diospyrin, a plant-derived bisnaphthoquinonoid, and its synthetic derivatives. Cancer Lett. 188, 85-93
  6. Chand, S., I. Lusunzi, D.A. Veal, L.R. Williams and P. Karuso. 1994. Rapid screening of the antimicrobial activity of extracts and natural products. J. Antibiot. 47, 1295-1304
  7. Chang, R. 2002. Bioactive polysaccharides from traditional chinese medicine herbs as anticancer adjuvants. J. Altern. Complement Med. 8, 559-565
  8. Chee, H.Y. 2002. In vitro Evaluation of the antifungal activity of propolis extract on Cryptococcus neoformans and Candida albicans. Mycology 30, 93-95
  9. Chen, X.M. and G.Y. Tian. 2003. Structural elucidation and antitumor activity of a fructan from Cyathula officinalis Kuan. Carbohydr. Res. 338, 1235-1241
  10. Franz, G. 1989. Polysaccharides in pharmacy: current applications and future concepts. Plant Med. 55, 493-497
  11. Ha, Y.L. and W.P. Michael. 1991. Naturally-occuring novel anticarcinogens: Conjugated dienoic derivatives of linoleic acid (CLA) (in Korean). J. Kor. Soc. Food Nutr. 20, 401-407
  12. Kim, H.S. and J.O. Shin. 1997. Isolation and antimicrobial activity of Xanthium strumarium L. extract. Kor. J. Appl. Microbiol. Biotechnol. 25, 183-188
  13. Kono, T., M. Watanabe, K. Koyama, T. Kishimoto, S. Fukushima, T. Sugimura and K. Wakabayashi. 1999. Cytotoxic activity of pierisin, from the cabbage butterfly, Pieris rapae, in various human cancer cell lines. Cancer Lett. 137, 75-81
  14. Lee, B.W. and D.H. Shin. 1991. Screening of natural antimicrobial plant extract on food spoilage microorganisms. Kor. J. Food Sci. Technol. 23, 200-204
  15. Lee, S.W., H.J. Kim and S. Hwangbo. 2001. Studies on the chemical characteristics of korean propolis. Kor. J. Food Sci. Ani. Resour. 21, 383-388
  16. Park, H.J., H.S. Young, J.O. Kim, S.H. Lee and J.S. Choi. 1991. A study on the chemical constituents of Orostachys japonicus A. Berger. Kor. J. Pharm. 22, 78-84
  17. Park, H.J., S.C. Leem, M.S. Lee and H.S. Young. 1994. Triterpene and steroids from Orostachys japonicus. Kor. J. Pharm. 25, 20-23
  18. Roberfroid, M.B. 1977. Health benefits of non-digestible oligosaccharides. Diet. Fiber Health Dis. 22, 211-219
  19. Taylor, K.A.C.C. 1995. A modification of the phenol sulfuric acid method of total sugar determination. Appl. Biochem. Biotechnol. 53, 207-214