Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Diagnosis of porcine reproductive and respiratory syndrome (PRRS) and its serological survey using the reverse transcription and polymerase chain reaction (RT-PCR) and ELISA

RT-PCR과 ELISA를 이용한 PRRS 진단 및 항체가 조사

  • Chu Keum-Suk (Jangsu-Branch, Jeonbuk Development & Livestock Research Institute) ;
  • Han Keu-Sam (Jangsu-Branch, Jeonbuk Development & Livestock Research Institute) ;
  • Han Jae-Cheol (Iksan-Branch, Jeonbuk Development & Livestock Research Institute) ;
  • Song Hee-Jong (Bio-Safety Research Institute, Chonbuk National University)
  • 추금숙 (전북축산진흥연구소장수지소) ;
  • 한규삼 (전북축산진흥연구소장수지소) ;
  • 한재철 (축산진흥연구소익산지소) ;
  • 송희종 (전북대학교 생체안전성연구소)
  • Published : 2004.12.01
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Abstract

The studies were performed for the PRRS antigen and antibody detection from breeding farms, artificial insemination(AI) center and growing farms in Jeonbuk province. 1. Specific PRRS primers were successfully amplified ORF6 617bp and ORF7 448 bp on agarose gel. 2. RT-PCR method has been establish by commercial kit and the thermal cycler program consisted of 30 cycles: $95^{\circ}C$ for 30 sec, $45^{\circ}C$ for 30 sec, and $72^{\circ}C$ for 45 sec. 3. The results of PRRS antibody test by ELISA method in AI centers were $6.6\%,\;53.3\%$ and breeding farms $65\%,\;65\%\;and\;38.7\%$, respectively. The serological positive of the antibody in gilt higher than sow. 4. The sero-positive of the PRRS antibody showed average $21\%$ in domestic farms, $56.2\%$ in breeding farms, and $29.9\%$ in AI center.

Keywords

References

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