Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Immobilization of Glucose Oxidase on Multi-Wall Carbon Nanotubes for Biofuel Cell Applications

  • JUNG SOO KEUN (School of Chemical Engineering and Bioengineering, College of Engineering, The University of Ulsan) ;
  • CHAE YOUNG RAE (School of Chemical Engineering and Bioengineering, College of Engineering, The University of Ulsan) ;
  • YOON JONG MOON (School of Chemical Engineering and Bioengineering, College of Engineering, The University of Ulsan) ;
  • CHO BYUNG WON (Eco-Nano Research Center, Korea Institute of Science and Technology) ;
  • RYU KEUN GARP (School of Chemical Engineering and Bioengineering, College of Engineering, The University of Ulsan)
  • Published : 2005.04.01
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Abstract

Glucose oxidase was immobilized on the carboxylated multi-wall carbon nanotubes (MWNT-COOHs) in the presence of a coulping reagent, 1-ethy1-3-(3-dimethylaminopropy1) carbodiimide. Significant amounts of glucose oxidase were also immobilized on MWNT-COOHs without the coupling reagent. Various conditions for the immobilization of glucose oxidase were optimized. Optimal pH for the maximal activity of the immobilized glucose oxidase shifted to 7 from the optimal pH of 6 for the maximal activity of free enzyme due to the carboxy1 groups on the surface of MWNT-COOHs. An electrode of graphite rod with a diameter of 6 mm was fabricated using the immobilized glucose oxidase. The cyclic voltammetry study of the enzyme electrode revealed that the oxidation of glucose and subsequent transfer of electrons from the oxidation of glucose to the electrode were possible by the immobilized glucose oxidase without a mediator, implying that the enzyme electrode can be utilized for the development of biofuel cells.

Keywords

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