Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Increased Production of Exoinulinase in Saccharomyces cerevisiae by Expressing the Kluyveromyces marxianus INU1 Gene Under the Control of the INU1 Promoter

  • KIM HEE EUN (Division of Biological Sciences, and Research Center for Industrial Development of Biofood Materials) ;
  • QIN RUI (Basic Science Research Institute, Chonbuk National University) ;
  • CHAE KEON SANG (Division of Biological Sciences, and Research Center for Industrial Development of Biofood Materials)
  • Published : 2005.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

Aspergillus niger inuE gene and Kluyveromyces marxianus INUI gene coding for exoinulinase were expressed in Saccharomyces cerevisiae under the control of K. marxianus INUI promoter. Recombinant S. cerevisiae expressing K. marxianus exoinulinase produced maximum 85 U/ml into culture medium, which was 9- to 14-fold higher than the activity produced by any other strain reported so far. In addition, K. marxianus INUI promoter produced 20- fold higher activity than S. cerevisiae glyceraldehydes phosphate dehydrogenase (GPD) promoter in S. cerevisiae.

Keywords

References

  1. Bergkamp, R. J. M., T. C. Bootsman, H. Y. Toschka, A. T. A. Mooren, L. Kox, J. M. A. Verbakel, R. H. Geerse, and R. J. Planta. 1993. Expression of an $\alpha-galactosidase$ gene under control of the homologous inulinase promoter in Kluyveromvces marxianus. Appl. Microbiol. Biotechnol. 40: 309- 317
  2. Brevnova, E. E., D. G. Kozlov, B. D. Efremov, and S. V. Benevolensky. 1998. Inulinase-secreting strain of Saccharomyces cerevisiae produces fructose. Biotechnol. Bioeng. 60: 492-497 https://doi.org/10.1002/(SICI)1097-0290(19981120)60:4<492::AID-BIT11>3.0.CO;2-K
  3. Ito, H., Y. Fukuda, K. Murata, and A. Kimura. 1983. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153: 163- 168
  4. Kim, H.-S., D. W. Lee, E. J. Ryu, T-B. Uhm, M.-S. Yang. J. B. Kim, and K.-S. Chae. 1999. Expression of the INU2 gene for an endoinulinase of Aspergillus ficuum in Saccharomyces cerevisiae. Biotech. Lett. 21: 621- 623 https://doi.org/10.1023/A:1005567403830
  5. Laloux. O., J. P. Cassatt, J. Delcour, J. Van Beeumen, and J. Vandenhaute. 1991. Cloning and sequencing of the inulinase gene of Kluyveromyces marxianus var. marxianus ATCC 12424. FEBS Lett. 289: 64- 68 https://doi.org/10.1016/0014-5793(91)80909-M
  6. Miller, G. L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal. Chem. 31: 426- 428 https://doi.org/10.1021/ac60147a030
  7. Moriyama, S., H. Tanaka, M. Uwataki, M. Muguruma, and K. Ohta. 2003. Molecular cloning and characterization of an exoinulinase gene from Aspergillus niger strain 12 and its expression in Pichia pastoris. J. Biosci. Bioeng. 96: 324-331 https://doi.org/10.1016/S1389-1723(03)90131-9
  8. Nehlin, J. O. and H. Ronne. 1990. Yeast MIG1 repressor is related to the mammalian early growth response and Wilms tumor finger proteins. EMBO J. 9: 2891- 2898
  9. Nehlin, J. O., M. Carlberg, and H. Ronne. 1991. Control of yeast GAL genes by MIG1 repressor: A transcriptional cascade in the glucose response. EMBO J. 10: 3373- 3377
  10. Ohta, K., S. Hamada, and T. Nakamura. 1993. Production of high concentrations of ethanol from inulin by simultaneous saccharification and fermentation using Aspergillus niger and Saccharomyces cerevisiae. Appl. Environ. Microbiol. 59: 729- 733
  11. Pandey, A., C. R. Soccol, P. Selvakumar, V. T Soccol, N. Krieger, and J. D. Fontana. 1999. Recent developments in Microbiol inulinases. Appl. Biochem. Biotechnol. 81: 35- 52 https://doi.org/10.1385/ABAB:81:1:35
  12. Park, S., H.-Y. Jeong, H.-S. Kim, M.-S. Yang, and K.-S. Chae. 2001. Enhanced production of Aspergillus ficuum endoinulinase in Saccharomyces cerevisiae by using the SUC2-deletion mutation. Enz. Microbial Technol. 29: 107-110 https://doi.org/10.1016/S0141-0229(01)00358-1