Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

Effects of Soybean Protein on Bone Mineral Density and Bone Mineral Content in Ovariectomized Rats

난소절제 쥐에서 콩단백질의 섭취가 골밀도와 골무기질 함량에 미치는 영향

  • Choi Mi-Ja (Department of Food and Nutrition, Keimyung University) ;
  • Jung Ji-Won (Department of Food and Nutrition, Keimyung University)
  • 최미자 (계명대학교 식품영양학과) ;
  • 정지원 (계명대학교 식품영양학과)
  • Published : 2005.05.01
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Abstract

The purpose of this study was to examine whether soybean protein isolate with rich isoflavones which was the same amount as used for earlier study prevents bone induced by ovarian hormone deficiency. Forty female Sprague-Dawley rats (body weight 210 $\pm$ 5 g) were divided into two groups, ovariectomy (OVX) and sham surgery groups, which were each randomly divided into two subgroups that were fed casein and soybean protein isolate with rich isoflavones (isoflavones 3_4 mg/g protein). The diets were fed for 9 weeks after operation. The results of this study indicate that body weight gain and food intake were higher in OVX groups than in SHAM groups regardless of diets. Weight gain were higher in soy isolate groups than in casein groups. Serum Ca concentration was lower in OVX rats than in SHAM rats when fed casein diet. Within the OVX groups, serum Ca concentration was higher in rats fed soy isolate diet than in rats fed casein diet. Serum ALP were higher in OVX groups than in SHAM groups. Within the OVX, soy isolate groups, indices of serum osteocalcin, were a trend for greater than those of the OVX casein group. Crosslink value were increased in ovariectomy groups. Spine BMD and femur BMD of ovariectomy groups were significantly lower than SHAM groups. However, soy isolate with rich isoflavones group in ovariectomy groups, spine BMC and femur BMC were significantly increased after 9 weeks. In conclusion, the soy protein with rich isoflavones was beneficial for bone in ovareiectomized rats and the results were similar to the findings of our previous study which showed a beneficial effect on bone in growing rats.

Keywords

References

  1. Korea National Statistical Office. Complete life Tables, 2004
  2. Park HS. Effects of soybean foods intake on menopausal symptoms, serum hormones, serum lipids, and bone metabolism in middle-aged women. Dissertation of Ph D. Ewha Womans University, Seoul, 2003
  3. Anderson LE, Garm J, Skouby SO, Jespersen J. Effects of hormone replacement therapy on hemostatic cardiovascular risk factors. Am J Obstet Gynecol 180: 283-289, 1999 https://doi.org/10.1016/S0002-9378(99)70201-2
  4. Setchell KDR, Borriello SP, Hulme P, Axelson M. Nonsteroidal estrogens of dietary origin: Possible roles in hormone dependent disease. Am J Clin Nutr 40: 569-578, 1984
  5. Washburn S, Burke GL, Morgan T, Anthony M. Effect of soy protein supplementation on serum lipoproteins, blood pressure and menopausal symptoms in perimenopausal women. Menopause 6: 7-13, 1999
  6. Song TT, Hendrich S, Murphy PA. Estrogenic activity of glycitein, a Soy Isoflavone. J Agric Food Chem 47: 1607-1610, 1999 https://doi.org/10.1021/jf981054j
  7. Arjmandi BH, Alekel L, Hollis BW, Amin D, Stacewicz-Sapuntzakis M, Guo P, Jukreja SC. Dietary soybean protein prevents bone loss in an ovariectomized rat model of osteoporosis. J Nutr 126: 161-167, 1996
  8. Alekel DL, Germain AS, Peterson CT, Hanson KB, Stewart JW, Toda T. Isoflavone-rich soy protein isolate attenuates bone loss in the lumbar spine of perimenopausal women. Am J Clin Nutr 72: 844-852,2000
  9. Toda T, Uesugi T, Hirai K, Nukaya H, Tsuji K, Ishida H. New 6-O-acyl isoflavone glycosides from soybeans fermented with Bacillus subtilis (natto): I. 6-O-succinylated isoflavone glycosides, and their preventive effects on bone loss in ovariectomized rats fed acalcium-deficient diet. Bioi Phann Bull 22: 1193-120I, 1999
  10. Deyhim F, Stoecker BJ, Brusewitz GH, Atjrnadi BH. The effects of estrogen depletion and isoflavone on bone metabolism in rats. Nutrition Research 23: 123-130, 2003 https://doi.org/10.1016/S0271-5317(02)00477-3
  11. Park YH. The effect of isoflavone supplementation on bone metabolism in ovariectomized SD rats. MS Thesis. Yonsei University, Seoul,2000
  12. Stephanie CB, Susanne NH, Beth MB, Maren H, Scott CM. Dietary soy protein maintains some indices of bone mineral density and bone formation in aged ovariectomized rats. J Nutr 133: 1244-1249,2003
  13. Choi MJ, Jo HJ. Effects of soy protein and isoflavones on bone mineral density in growing female rat. Kor J Nutr 36: 359-367, 2003
  14. Kim MS. Beneficial effect of soy isoflavone on bone loss and hyperlipidemia in ovariectomized rats. Dissertation of Ph. D, Seoul National University, Seoul, 1999
  15. Kang YJ. Effects of isoflavones on bone mineral density and bone mineral content in ovariectomized rats. MS Thesis. Keimyung University, Daegu, 2004
  16. Kim WY, Choi HK, Lee HS. The effects of dietary ca levels on ca and skeletal metabolism in ovariectomized rats of different ages. Kor J Nutr Soc 31(4): 716-728, 1998
  17. Kim YK. Effects of soybean isoflavones on the bone metabolism in ovariectomized growing rats. MS Thesis. Hanyang University, Seoul, 2002
  18. Jang HR. The effect of soy isoflavone supplementation on the factors relating to vascular disease in ovariectomized and hyperlipidemic rats. MS Thesis. Seoul National University, Seoul, 2002
  19. Wronski TJ, Cinton M, Doherty AL, Donn LM. Estrogen treatment prevents osteopenia and depresses bone turnover in ovariectomizedrats. Endocrinol 123: 681-868,1988 https://doi.org/10.1210/endo-123-2-681
  20. Utian WH. Biosynthesis and physiologic effects of estrogen and pathophysiologic effects of estrogen deficiency: a review. Am J ObestetGynecol 161: 1828-1831,1989
  21. Gordin JM, Siiteri PK, Mcdonald PC. Source of estrogm production in postmenopausal women. J Clin Endocrin Meta 76: 207-218,1993 https://doi.org/10.1210/jc.76.1.207
  22. Williams JP, Iordan SE, Barnes S, Blair HC. Tyrosine kinase inhibitor effects on avian osteoclastic acid transport. Am J Clin Nutr 68(6S): 1369-1374, 1998
  23. Chung LW, Gleave ME, Hsi JT, Hong SJ, Zhau HE. Reciprocal mesenchymal epithelial interaction affecting prostate turnor growth and hormonal responsiveness. (review) Cancer Surv 11: 91-121,1991
  24. Lee MH, Park YH, Oh HS, Kwak TS. Isoflavone Content in Soybean and its Processed Products. J Kor Food Sci Nutr 34(3): 365-369, 2002
  25. Mituka BM, Rawnsley HM. Clinical biochemical and hemotologycal reference value in nomal experimental animals and normal humans. 2nd edition, Masson, New York, p160, 1987
  26. Barzel US, Massey LK. Excess dietary protein can adversely affect bone. J Nutr 128: 1051-1053, 1998
  27. Riis B, Thomsen K, Christiansen C. Does calcium supplementation prevent postmenopausal bone loss? N Engl J Med 316: 173-177, 1987 https://doi.org/10.1056/NEJM198701223160401
  28. Fanti O, Faugere MC, Gang Z, Schmidt J, Cohen D, Malluche HH. Systematic administration of genistein partially prevents bone loss in ovariectomized rats in a nonestrogen-like mechanism. Am J Clin Nutr 68(suppI): 1517s(abstr), 1998
  29. Scheiber MD, Liu JH, Subbiah MTR, Rebar RT, Setchell KDR. Dietary inclusion of whole soy foods results insignificant reductions in clinical risk factors for osteoporosis and cardiovascular disease in normal postmenopausal women [NAMS Fellowship Findings]. Menopause 8 (5) : 384-392, 2001 https://doi.org/10.1097/00042192-200109000-00015
  30. Lees DJ, Ginn TA. Soy protein isolate diet does not prevent increased cortical bone turnover in ovariectomized macques. Clcif Tissue Int 62: 557-558, 1998 https://doi.org/10.1007/s002239900479
  31. Uesugi T, Toda T, Tsuji K, Ishida H. Comparative study on reduction of bone loss and lipid metabolism abnormality in ovariectomized rats by soy isoflavones, daidzin, genistin, and glycitin, Bioi Pharm Bull 24 (4) : 368-372,2001 https://doi.org/10.1248/bpb.24.368
  32. Cecchettin M, Bellomitti S, Cremonesi G, Solimeno LP, Torri G. Metabolic and bone effects afteradministration of ipriflavone and salmon calcitonin in postmenopausal osteoporosis. Biom & Pharm 49:465-468,1995 https://doi.org/10.1016/0753-3322(96)82691-6
  33. Jeon HS. Effects of soy protein and isoflavones on bone mineral content and bone mineral density in growing rats consuming a low calcium diet. MS Thesis. Keimyung University, Taegu, 2003
  34. Funada M, Kawashima T, Yano H, Kawashima R. Effects of a high protein diet on bone formation and calcium metabolism in rats. J Nutr Sci Vitaminol 36: 559-567, 1990
  35. Lee DH, Sung CJ. Effect of soy isoflavone supplementation on bone metabolism marker and urinary mineral excretion in post- menopausal women. Kor J Nutr Soc 36 (5) : 476-482, 2003
  36. Uesugi T, Fukui Y, Yamori Y. Beneficial effects of soybean isoflavone supplementation on bone metabolism and serum lipids in postmenopausal Japanese women: A four-week study. Original Research 21 (2) : 97-102, 2002
  37. Sung CJ, Baek SK, Lee HS, Kim MH, Choi SH, Lee SY, Lee DH. A study of body anthropometry and dietary factors affecting bone mineral density in Korean pre - and postmenopausal women. J Kor Food Sci Nutr 30 (1): 159-167, 2000
  38. Lee YS, Kim EM. Effect of ovariectomy and dietary calcium levels on bone metabolism in rats fed low calcium diet during growing period. Kor J Nutr Soc 31: 279-288, 1998
  39. Potter SM, Baum JA, Teng H, Stillman RJ, Shay NF, Erdman JWJ. Soy protein and isoflavones: their effects on blood lipids and bone density in postmenopausal women. Am J Clin Nutr 68 (suppl) : 1375-1379, 1998
  40. Rice MM, LaCroix AZ, Lampe JW. Soy consumption and bone mineral density in older Japanese American women in King Country, Washington. J Nutr 130: 685, 2000
  41. Dalais FS, Rice GE, Wahlqvist ML, Grehan M, Murkies AL, Medley G, Ayton R, Strauss BJ. Effects of dietary phytoestrogens in postmenopausal women. Climacteric 1: 124-129,1998 https://doi.org/10.3109/13697139809085527
  42. Somekawa Y, Chiguchi M, Ishibashi T, Aso T. Soy intake related on menopausal symptoms, serum lipids, and bone mineral density in postmenopausal Japanese women. Obstet Gynecol 97 (1) : 109-115, 1997 https://doi.org/10.1016/S0029-7844(00)01080-2
  43. Atkinson C, Compston AJ, Day NE, Dowsett M, Bingham SA. The effects of phytoestrogen isoflavones on bone density in women: a double-blind, randomized, placebo-controlled trial. Am J Clin Nutr 79: 326-333, 2004