Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Antioxidant Activity of Monascus Pigment of Monascus purpureus P-57 Mutant

Monascus purpureus P-57 변이주가 생산하는 홍국색소의 항산화효과

  • Park Chi Duck (Department of Microbiology, Keimyung University) ;
  • Jung Hyuck Jun (Department of Microbiology, Keimyung University) ;
  • Lee Hang Woo (Traditional Microorganism Resources Center, Keimyung University) ;
  • Kim Hyun Soo (Department of Microbiology, Keimyung University) ;
  • Yu Tae Shick (Department of Microbiology, Keimyung University)
  • 박치덕 (계명대학교 미생물학과) ;
  • 정혁준 (계명대학교 미생물학과) ;
  • 이항우 (전통미생물자원 개발 및 산업화 연구센터) ;
  • 김현수 (계명대학교 미생물학과) ;
  • 유대식 (계명대학교 미생물학과)
  • Published : 2005.06.01
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Abstract

Antioxidant activity of monascus pigment of Monascus purpureus P-57 mutant was studied. Methanol extract from monascus pigment was separated into five organic solvent fractions; hexane, chloroform, ethyl acetate, butanol and water fractions. Hexane fraction showed the highest free radical scavenging effect on 1,l-diphenyl-2-picryl hydrazyl(DPPH), and the strongest inhibitory effect against xanthine oxidase, followed by chloroform fraction. But butanol and water fraction did not show inhibitory effect against the enzyme. Lineweaver-Burk plot showed that hexane fraction inhibited xanthine oxidase by non-competitive mode.

Monascus purpureue KCCM 60016으로부터 색소생성 이 우수한 변이주 P-57이 생성한 홍국색소의 항산화 활성을 조사하였다. 홍국색소는 chloroform에 아주 잘 추출되며, 특히 hexane에서는 황색색소가 특이적으로 높게 추출되었다. DPPH radical소거 효과는 hexane분획, chloroform 분획, ethyl acetate분획, butanol 분획, water 분획의 순으로 높게 나타났다. Xanthine oxidase저해효과는 hexane분획, chloroform분획, ethyl acetate 분획의 순으로 높게 나타났으며, hexane 분획물의 경우 5 ppm에서 $41.2\%$, 50 ppm에서 $82.4\%$로 높은 저해율을 보였으며, 저해기작은 비경쟁적 저해였다.

Keywords

References

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