Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
권호 표지

Anti-Oxidative and Inhibitory Effect of Saussurea involucrata on MMP-1 in UVA-irradiated Human Dermal Fibroblast

설련의 항산화능와 사람 섬유아세포에서 UVA에 의한 MMP-1발현 저해효과

  • 심관섭 (한불화장품㈜ 기술연구소) ;
  • 김진화 (한불화장품㈜ 기술연구소) ;
  • 나영 (한불화장품㈜ 기술연구소) ;
  • 이동환 (한불화장품㈜ 기술연구소) ;
  • 이범천 (한불화장품㈜ 기술연구소) ;
  • ;
  • 표형배 (한불화장품㈜ 기술연구소)
  • Published : 2005.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

In order to investigate the effects of Saussurea involucrata on (relationship between) aging (and Saussurea involucrata), we examined the activities of antioxidation, in vitro MMP inhibition and UVA-induced MMP-1 expression in human dermal fibroblasts. S. involucrata showed scavenging activities radicals and reactive oxygen species (ROS) with the $IC_{50}$ values of $3.89{\mu}g/mL$ against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and $67.29{\mu}g/mL$ against superoxide radicals in the xanthine/xanthine oxidase system, respectively. At the concentration of $1000{\mu}g/mL$, S. involucrata showed 93.27% inhibition on lipid peroxidation of linoleic acid. S. involucrata inhibited the activities of MMP-1 in a does-dependent manner and the $IC_{50}$ value calculated from semi-log plots was $97.18{\mu}g/mL$. Also, UVA induced MMP expression in human dermal fibroblasts was reduced 42.86% by treatment with S. involucrata, and MMP-1 mRNA expression was reduced in a dose-dependent manner. Therefore S. involucrata was able to significantly inhibit MMP expression in protein and mRNA level. All these results suggested that S. involucrata might act as an anti-aging agent by antioxidation and reducing UVA-induced MMP-1 production.

노화와 연관하여 설련의 항산화 효과, in vitro MMP 활성 저해능, 자외선 조사에 의해 유도된 MMP-1 발현에 대한 영향을 사람섬유아세포를 이용하여 확인하였다. 설련의 항산화 효과를 알아보기 위하여 DPPH radical과 superoxide anion radical 소거효과를 측정하였다. 그 결과 DPPH radical 소거능의 $IC_{50}$ 값은 $3.89{\mu}g/mL$이고, xanthine/xanthine oxidase에 의한 superoxide anion radical 제거능의 $IC_{50}$ 값은 $67.29{\mu}g/mL$이었다. 설련 $1000{\mu}g/mL$에서 93.27%의 지질과산화 저해효과를 나타내었다. MMP-1의 효소활성 저해 효과는 농도 의존적으로 활성을 억제하였으며, $IC_{50}$ 값은 $97.18{\mu}g/mL$이다. 또한 자외선 조사에 의해 사람 섬유아세포에서 발현되는 MMP-1에 대해 단백질의 양적인 변화는 42.86% 감소되었으며, 설련에 의해 농도 의존적으로 MMP-1 mRNA의 발현량도 감소되었다. 이러한 실험결과를 통하여 설련은 항산화 효과뿐만 아니라 자외선 조사에 의해 유도되는 MMP-1 단백질 발현과 mRNA 유전자 수준에서의 조절이 가능함을 확인하였다. 결론적으로 설련은 항산화와 자외선으로부터 생성되는 MMP-1의 발현을 저해함으로써 노화와 따른 피부를 보호하는 항노화 소재로서의 응용가능성을 확인하였다.

Keywords

References

  1. A Oikarinen, The aging of skin: chronoaging versus photoaging, Photodermatol. Photoimmunol. Photomed., 7, 3 (1990)
  2. B. A. Gilchrest, Skin aging and photoaging: an overview, J. Am. Acad Dermatol., 21, 610 (1989) https://doi.org/10.1016/S0190-9622(89)70227-9
  3. J. Uitto, M. J. Fazio, and D. R. Olsen, Molecular mechanism of cutaneous aging, J. Am. Acad. Dermatol., 21, 614 (1989) https://doi.org/10.1016/S0190-9622(89)70228-0
  4. A. M. Klingman and R. M. Laver, Cutaneous aging: the differences between intrinsic aging and photoaging, J. Cutan. Aging. Cosmet. Dermatol., 1, 5 (1988)
  5. S. K Karin, B. Peter, W. Jutta, H. Gernot, M. Weijan, K. Lale, M. Christian, and W. Meinhard, Photoaging of the skin from phenotype to mechanisms, Experimen. Gerontol, 35, 307 (2000) https://doi.org/10.1016/S0531-5565(00)00098-X
  6. J. Y. Seo, H. R. Choi, G. E. Rhie, C. S. Youn, W. W. Choi, J. A. Kim, J. H. Chung, K. H. Kim, K. H. Cho, and H. C. Eun, The effect of retinoic acid and vitamin C on the expression of the procollagen a 1(I), tropoelastin, and MMP-1 in human dermal fibroblast, Kor. J. Invest. Dermtaol., 8, 23 (2001)
  7. W. Meinhard, T. B. lliana, N. Lale, M. Wenjian, A. S. Lars, R. W. Ziba, S. Jutta, and S. K Karin, Solar UV irradiation and dermal photoaging, J. Photochem. Photobio. B., 63, 41 (2001) https://doi.org/10.1016/S1011-1344(01)00194-4
  8. G. J. Fisher, S. W. Kang, and J. J. Voorhees, Retinoic acid inhibits induction of c- Jun protein by ultraviolet radiation that occurs subsequent to activation of mitogen-activated protein kinase pathways in human skin in vivo, J. Clin Invest., 101, 1432 (1998) https://doi.org/10.1172/JCI2153
  9. G. J. Fisher and J. J. Voorhees, Molecular mechanisms of photoaging in human skin in vivo and their prevention by all-trans retinoic acid, Photochem. Photobiol., 69, 154 (1999) https://doi.org/10.1111/j.1751-1097.1999.tb03268.x
  10. M. Barthelman, W. B. Bair, K. K. Stickland, W. Chen, B. N. Tmmermann, S. Valcic, Z. Dong, and G. T. Bowden, (- )-epigallocatechin-3-gallate inhibition of ultraviolet B-inducecl AP-1 activity, Carcinogenesis, 19, 3201 (1998)
  11. G. J. Fisher, S. C. Datta, H. S. Talwar, Z. Q. Wang, J. Varani, S. W. Kang, and J. J. Voorhees, Molecular basis of sun-induced premature skin ageing and retinoid antagonism, Nature, 379, 335 (1996) https://doi.org/10.1038/379335a0
  12. S. Konno, Maitake D-fraction. Alternative & Complementary Therapies, 7, 102 (2001) https://doi.org/10.1089/10762800151125137
  13. N. Kodama, K. Komuta, N. Sakai, and H. Nanba, Effects of D-fraction, a polysaccharide from Grifola frondosa on tumor growth involve activation of NK cells, BioI. Pharm. Bull., 25, 1647 (2002) https://doi.org/10.1248/bpb.25.1647
  14. N. Kodama, Y. Murata, and H. Nanba, Administration of a polysaccharide from Grifola frondosa stimulates immune function of normal mice, J. Med. Food, 7, 141 (2004) https://doi.org/10.1089/1096620041224012
  15. M. S. Blois, Artioxidant determinations by the use of a stable free radical, Nature, 181, 1199 (1958) https://doi.org/10.1038/181958a0
  16. K. Furuno, T. Akasako, and N. Sugihara, The contribution of the pyrogallol moiety to the superoxide radical scavenging activity of flavonoids, Biol. Pharm. Bull., 25, 19 (2002) https://doi.org/10.1248/bpb.25.19
  17. A. M. Romero, M. M. Doval, M. A. Sturla, and M. A. Judis, Antioxidant properties of polyphenolcontaining extract from soybean fermented with Saccharomyces cerevisia, Eur. J. Lipid Sci. Technol., 106, 424 (2004) https://doi.org/10.1002/ejlt.200400953
  18. T. Mosmann, Rapid colorimetric assay for the cellular growth and survival: application to proliferation and cytotoxic assay, J. Immun. Methods, 65, 55 (1983) https://doi.org/10.1016/0022-1759(83)90303-4
  19. A. Kitahara, U. Matsumoto, H. Ueda, and R. Ueoka, R. A remarkable anti oxidation effect of natural phenol derivatives on the autoxidation of $\gamma$ -irradiated methyl linolate, Chem. Pharm. Bull., 40, 2208 (1992) https://doi.org/10.1248/cpb.40.2208
  20. T. Hatano, Constituents of natural medicines with scavenging effects on active oxygen species-Tannins and related polyphenols, Natural Medicines, 49, 357 (1995)
  21. H. Masaki, S. Sakaki, T. Atsumi, and H. Sakurai, Activeoxygen scavenging activity of plant extracts, BioI. Pharm. Bull., 18, 162 (1995) https://doi.org/10.1248/bpb.18.162
  22. P. Kuppusamy and J. L. Zweier, Characterization of free radical generation by xanthine oxidase, J. Biol. Chem., 264, 9880 (1989)
  23. S. H. Cho, Lipid peroxidation and nutrition, Korean J. Lipidology, 3, 23 (1993)
  24. J. H. Chun, S. W. Kang, J. Varani, J. Lin, G. J. Fisher, and J. J. Voorhees, Decreased extracellular signal regulated kinase and increased stress activated MAP kinase activities in aged human skin in vivo, J. Invest. Dermatol., 115, 177 (2000) https://doi.org/10.1046/j.1523-1747.2000.00009.x
  25. J. G. Kim, J. G. Hwang, Y. K. Cho, Y. G. Han, Y. J. Jeon, and K. H. Yang, Protective effects of (- )-epigallocatechin- 3-gallate on UV A-and UVB-induced skin damage, Skin Pharmacol. Appl. Skin Physiol., 14, 11 (2001) https://doi.org/10.1159/000056329
  26. K. Scharffetter, M. Wlaschek, and A. Hogg, UV A irradiation induces collagenase in human dermal fibroblasts in vitro and in vivo, Arch. Dermatol. Res., 283, 506 (1991) https://doi.org/10.1007/BF00371923
  27. M. Wlaschek, K. BoIsen, G. Herrmann, A. Schwarz, F. Wilmroth, P. C. Heinrich, G. Goerz, and K. Scharffetter, UVA induced autocrine stimulation of fibroblast derived collagenase by IL -6: a possilbe mechanism in dermal photodamage?, J. Invest. Dermatol., 101, 164 (1993) https://doi.org/10.1111/1523-1747.ep12363644
  28. H. C. Kim, J. S. Yang, Y. S. Chae, K. S. Suh, and S. T. Kim, The effect of all-trans-retinoic acid and ursolic acid on the ultraviolet a radiation induced AP-1 (Fos/Jun) activity in cultured human dermal fibroblasts, Kor. J. Invest. Dermtaol., 35, 1136 (1997)
  29. M. Barthelman, W. B. Bair, K. K. Stickland, W. Chen, B. N. Timmermann, S. Valcic, Z. Dong, and G. T. Bowden, (- )-epigallocatechin-3-gallate inhibition of ultraviolet B-induced AP-1 activity, Carcinogenesis, 19, 2201 (1998) https://doi.org/10.1093/carcin/19.12.2201
  30. G. J. Fisher, H. S. Talwar, J. Lin, P. Lin, P. McPhillips, Z. Q. Wang, X. Li, Y. Wan, S. W. Kang, and J. J Voorhees, Retinoic acid inhibits induction of c-Jun protein by ultraviolet radiation that occurs subsequent to activation of mitogen activated protein kinase pathways in human skin in vivo, J. Clin. Invest., 101, 1432 (1998) https://doi.org/10.1172/JCI2153