Effect of Feeding Frequency of Extruded Diets Containing Different Macro-nutrient Levels on Apparent Nutrient Digestibility in Grower Flounder Paralichthys olivaceus

영양소 함량이 다른 부상 배합사료의 공급횟수가 육성기 넙치의 영양소 소화율에 미치는 영향

  • Seo, Joo-Young (Faculty of Marine Bioscience and Technology, Kangnung Notional University) ;
  • Choi, Kyoung-Hyun (Faculty of Marine Bioscience and Technology, Kangnung Notional University) ;
  • Choi, Jin (Faculty of Marine Bioscience and Technology, Kangnung Notional University) ;
  • Lee, Sang-Min (Faculty of Marine Bioscience and Technology, Kangnung Notional University)
  • 서주영 (강릉대학교 해양생명공학부) ;
  • 최경현 (강릉대학교 해양생명공학부) ;
  • 최진 (강릉대학교 해양생명공학부) ;
  • 이상민 (강릉대학교 해양생명공학부)
  • Published : 2005.08.25

Abstract

Two feeding trials were carried out to investigate apparent nutrient digestibility of flounder fed experimental diets containing different levels of macro-nutrients by satiation feeding rate (Exp-1) and feeding frequency (Exp-2). Triplicate groups of fish averaging 280 g were fed three experimental diets which contained different levels of carbohydrate, protein and lipid by two feeding regimes (satiation and 80% satiation) and four feeding frequencies (three meals a day, two meals a day, one meal a day and one meal every two days). Feces were collected using a fecal collection column attached to fish rearing tanks for 6 weeks. Apparent digestibilities of dry matter, protein, lipid, energy and carbohydrate were not affected by feeding satiation rate in Exp-1. Apparent protein digestibility was not affected by feeding frequency, whereas affected by dietary composition in Exp-2. Apparent protein digestibility of fish fed a high-protein diet showed a tendency to become higher compared to that of fish fed high-carbohydrate diet and high-lipid diet at the same feeding frequency. Apparent lipid digestibility was not affected by dietary composition, however, affected by feeding frequency. Apparent digestibilities of energy and carbohydrate were affected by both dietary composition and feeding frequency. Apparent digestibities of energy and carbohydrate in fish fed the high-protein diet showed a tendency to become higher compared to that of fish fed the high-carbohydrate diet and high-lipid diet at the same feeding frequency. Apparent digestibities of energy and carbohydrate tended to decrease with increasing of feeding frequency at the same dietary composition.

평균체중 280 g의 넙치를 대상으로 탄수화물, 단백질 및 지질 함량이 다른 3종류의 EP사료(HC, HP및 HL)를 제조하여, 사료 공급율과 공급휫수를 달리하여 영양소 소화율을 조사하였다. 사료의 공급율에 따른 실험(실험 1)은 파 사료별로 1일 1회 만복 공급구와 만복의 80% 공급구로 설정하였다. 사료 공급횟수에 따른 실험(실험 2)에서는 사료의 공급휫수를 각 사료마다 2일 1회, 1일 1회, 1일 2회 및 1일 3회로 설정하였다. 사료 공급율을 달리한 실험 1에서 넙치의 건물, 단백질, 지질, 에너지 및 NFE 소화율은 사료 조성과 공급율에 영향을 받지 않았다. (P>0.05).사료 공급횟수를 달리한 실험 2리 건물 소화율은 동일한 공급횟수에서 HP 공급구가 HC와 HL 공급구와 비교하여 높은 소화율을 보였다. 단백질 소화율은 사료 조성에 영향을 받아 HP 공급구가 수치상으로 높은 값을 보였으나 실험구간에 유의차는 없었다(P>0.05).지질 소화율은 사료 공급횟수에 영향을 받아 1일 2회와 3회 공급구에서 감소하는 경향을 보였다. NFE 소화율은 사료 조성과 공급횟수에 경향을 받았으며, 동일한 공급횟수에서 1일 3회 공급구를 제외한 실험 구에서 HP 공급구의 소화율이 높은 경향을 보였다. 그리고 동일한 사료에서는 2일 1회에서 1일 2회까지 공급횟수가 증가하면서 NFE 소화율이 낮아지는 경향을 보였다. 에너지 소화율은 동일한 공급 횟수에서 1일 1회 공급구를 제외하고 HP를 공급한 실험구가 다른 실험구에 비해 높았고, HP 공급구들 간에는 사료공급횟수에 영향을 받지 않았다.

Keywords

References

  1. Aksnes, A., 1995. Growth, feed efficiency and quality of salmon (Salrno salar) given feeds with different ratios of carbohydrate and protein. Aquacult. Nutr., 1,241-248 https://doi.org/10.1111/j.1365-2095.1995.tb00050.x
  2. Anderson, J. S., A. J. Jackson, A. J. Matty and B. S. Capper, 1984. Effects of dietary carbohydrate and fiber on the tilapia Orechrornis niloticus (Linn.). Aquaculture, 37, 303-314 https://doi.org/10.1016/0044-8486(84)90296-5
  3. AOAC, 1990. Official Methods of Analysis. 15th edition. Association of Official Analytical Chemists. Arlington, Virginia, USA. 1298 pp
  4. Austreng, E., A. Skrede and A. Eldegard, 1979. Effect of dietary fat source on the digestibility of fat and fatty acids in rainbow trout and mink. Acta Agric. Scand., 29, 119-126 https://doi.org/10.1080/00015127909435220
  5. Beamish, F. W. G and T. E. Medland, 1986. Protein sparing effects in large rainbow trout,Salrno gairdneri. Aquaculture, 55,35-42 https://doi.org/10.1016/0044-8486(86)90053-0
  6. Cho, C. Y. and S. J. Slinger, 1979. Apparent digestibility measurement in feed stuffs for rainbow trout. In: J. H. Halver and K. Tiews. (eds.), Finfish Nutrition and Fishfeed Technology, Vol. II. Heeneman, Berlin, pp. 239-247
  7. De Silver, S. S., R. M. Gunasekera and K. F. Shim, 1991. Interactions of varying dietary protein and lipid levels in young red tilapia: evidence ofprotein sparing. Aquaculture, 95, 305-318 https://doi.org/10.1016/0044-8486(91)90096-P
  8. Duncan, D. B., 1955. Multiple-range and multiple F tests. Biometrics, 11, 1-42 https://doi.org/10.2307/3001478
  9. Furuukawa, A. and H. Tsukahara, 1966. On the acid digestion method for the detennination of chromic oxide as an index substance in the study of digestibility of fish feed. Bull. Jpn. Soc. Sci. Fish., 32, 502-506 https://doi.org/10.2331/suisan.32.502
  10. Grisdale-Helland, B. and S. J. Hellannd, 1997. Replacement of protein by fat and carbohydrate in diets for Atlantic salmon (Salrno salar) at the end of the freshwater stage. Aquaculture, 139, 157-163 https://doi.org/10.1016/0044-8486(95)01145-5
  11. Hajen, W. E., D. A. Higgs, R. M. Beames and B. S. Dosanjh, 1993. Digestibility of various feedstuffs by post-juvenile chinook salmon (Oncorhynchus tshawytscha) in sea water. 2. Measurement of digestibility. Aquaculture, 112, 333-348 https://doi.org/10.1016/0044-8486(93)90394-E
  12. Hastings, W. H., 1969. Nutritional score. In; O. W. Neuhaus and J. E. Halver (eds.), Fish in research. Academic press, New York, USA. pp. 263-292
  13. Hemre, G-I., O. Lie, E. Lied and G Lambertsen, 1989. Starch as an energy source in feed for cod (Gadus rnorhua): digestibility and retention. Aquaculture, 80, 261-270 https://doi.org/10.1016/0044-8486(89)90174-9
  14. Hemre, G-I., K. Sandnes, O. Lie, O. Torrissen and R. Waagbo, 1995. Carbohydrate nutrition in Atlantic salmon: I. Growth and feed utilization. Aquacult. Res., 26, 149-154 https://doi.org/10.1111/j.1365-2109.1995.tb00896.x
  15. Henken, A. M., D. W. Kleingeld and P. A. T. Tijssen, 1985. The effect of feeding level on apparent digestibility of dietary dry matter, crude protein and gross energy in the African catfish Clarias gariepinus (Burchell 1822). Aquaculture, 51, 1-11 https://doi.org/10.1016/0044-8486(85)90235-2
  16. Hillestad, M., F. Johnsen and T. Aasgaard, 2001. Protein to carbohydrate ratio in high-energy diets for Atlantic salmon (Sa/rno salar L.). Aquacult. Res., 32, 517-529 https://doi.org/10.1046/j.1365-2109.2001.00569.x
  17. Hilton, J. W., J. I. Atkinson and S. Slinger, 1983. Effect of increased dietary fiber on the growth of rainbow trout (Salrno gairdneri). Can. J. Fish. Aquacult. Sci., 40, 81-85 https://doi.org/10.1139/f83-012
  18. Holmgren, S., D. J. Grove and D. J. Fletcher, 1983. Digestion and control of gastrointestinal motility. In: J. C. Rankin and R. T. Dugan (eds.), Control Processes in Fish Physiology. Wiley, New York, NY, USA, pp. 23-40
  19. Kikuchi, K., 1999. Use of defatted soybean meal as a substitute for fish meal in diets of Japanese flounder (Paralichthys olivaceus). Aquaculture, 179, 3-11 https://doi.org/10.1016/S0044-8486(99)00147-7
  20. Kim, K. D., S.-M. Lee, H. G Park, S. C. Bai and Y. H. Lee, 2002. Essentiality of dietary n-3 highly unsaturated fatty acids in juvenile Japanese flounder (Paralichthys olivaceus). J. World Aquacult. Soc., 33, 432-440 https://doi.org/10.1111/j.1749-7345.2002.tb00022.x
  21. Kim, G-D., H.-S. Jang, J.-Y. Seo and S.-M. Lee, 2005. Effect of feeding frequency of extruded pellet on growth and body composition ofjuvenile flounder, Paralichthys olivaceus during the winter season. J. Aquacult., 18, 31-36
  22. Kim, S. M., S.-M. Lee and B.-D. Yoon, 2003. Effect offennented food garbage in diet on growth and body composition ofjuvenile flounder (Paralichthys olivaceus). J. Fish. Sci. Tech., 6, 45-50 https://doi.org/10.5657/fas.2003.6.2.045
  23. Larsen, F. M., M. N. Wilson and P. J. Moughan, 1994. Dietary fiber viscosity and amin acid digestibility, proteolytic digestive enzyme activity and digestive organ weights in growing rats. J. Nutr., 124, 833-841
  24. Lee, S.-M., 1997. Effects of feeding rates on growth, feed frequency and body composition of the juvenile Korean rockfish (Sebastes schlegeli). Kor. J. Anim. Nutr. Feed, 21, 327-334
  25. Lee, S.-M., 2002. Apparent digestibility coefficients of various feed ingredients for juvenile and grower rockfish (Sebastes schlegeli). Aquaculture, 202, 79-95
  26. Lee, S.-M., C. S. Park and I. C. Bang, 2002. Dietary protein requirement of young Japanese flounder Paralichthys olivaceus fed isocaloric diets. Fish. Sci., 68, 158-164 https://doi.org/10.1046/j.1444-2906.2002.00402.x
  27. Lee, S.-M., C. H. Seo and Y. S. Cho, 1999. Growth ofthe juvenile olive flounder (Paralichthys olivaceus) fed the diets at different feeding frequencies. J. Kor. Fish. Soc., 32, 18-21
  28. Lee, S.-M., K.-D. Kim and S. P. Lall, 2003. Utilization of glucose, maltose, dextrin and cellulose by juvenile flounder (Paralichthys olivaceus). Aquaculture, 221, 427-438 https://doi.org/10.1016/S0044-8486(03)00061-9
  29. Lee, S.-M., S. H. Cho and K. D. Kim, 2000a. Effects of dietary protein and energy levels on growth and body composition of juvenile flounder Paralichthys olivaceus. J. World Aquacult. Soc., 31, 306-315 https://doi.org/10.1111/j.1749-7345.2000.tb00882.x
  30. Lee, S.-M., S. H. Cho and D. J. Kim, 2000b. Effects of feeding frequency and dietary energy level on growth and body composition ofjuvenile flounder Paralichthys olivaceus. Aquacult. Res., 31, 917-921 https://doi.org/10.1046/j.1365-2109.2000.00505.x
  31. Lee, S.-M., U.-G Hwang and S. H. Cho, 2000c. Effects offeeding frequency and dietary moisture content on growth, body composition and gastric evacuation of juvenile Korean rockfish (Sebastes schlegeli). Aquaculture, 187, 399-409 https://doi.org/10.1016/S0044-8486(00)00318-5
  32. McGoogan, B. B. and R. C. Reigh, 1996. Apparent digestibility of selected ingredients in red drum (Sciaenops acellatus) diets. Aquaculture, 16, 39-46 https://doi.org/10.1016/0044-8486(79)90170-4
  33. Morales, A. E., G Cardenete, M. De la Higuera and A. Sanz, 1994. Effect of dietary protein source on growth, feed conversion and energy utilization in rainbow trout Oncorhynchus mykiss. Aquaculture, 124, 117-126 https://doi.org/10.1016/0044-8486(94)90367-0
  34. Shah, N., M. T. Atallah, R. P. Mahoney and P. L. Pellet, 1982. Effect of dietary fiber components of fecal nitrogen excretion and protein utilization in growing rats. J. Nutr., 112, 658-666 https://doi.org/10.1093/jn/112.4.658
  35. Smith, L. S., 1989. Digestive functions in teleost fishes. In: J. E. Halver (ed.), Fish Nutrition. Academic Press, London, UK. pp. 332-411
  36. Smith, R. W. and R. T. Lovell, 1973. Determination of apparent protein digestibility in feds for channel catfish. Trans. Am. Fish. Soc., 102, 831-835 https://doi.org/10.1577/1548-8659(1973)102<831:DOAPDI>2.0.CO;2
  37. Storebakken, T., K. D. Shearer, S. Refstie, S. Lagocki and J. McCool, 1998. Interaction between salinity, dietary carbohydrate source and carbohydrate concentration on the digestibility ofmacronutrients and energy in rainbow trout (Oncorhynchus mykiss). Aquaculture, 163, 347-359 https://doi.org/10.1016/S0044-8486(98)00259-2
  38. Sugiura, S. H., F. M. Dong, C. K. Rathbone and R. W. Hardy, 1998. Apparent digestibility and mineral availabilities in various feed ingredients for salmonids feeds. Aquaculture, 159, 177-202 https://doi.org/10.1016/S0044-8486(97)00177-4
  39. Sullivan, J. A. and R. C. Reigh, 1995. Apparent digestibility of selected feedstuffs in diets for hybrid striped bass (Marone saxatilis man x Marone chrysops woman). Aquaculture, 138,313-322 https://doi.org/10.1016/0044-8486(95)01071-8
  40. Takeuchi, T., Y. Shina, T. Watanabe, S. Sekiya and K. Imaizumi, 1992. Suitable protein and lipid levels in diet for fingerlings of yellowtail. Nippon Suisan Gakkaishi, 58, 1333-1339 https://doi.org/10.2331/suisan.58.1333