Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
권호 표지

Tylosin Production by Mutant Resistant to Oleic Acid

Oleic Acid 내성균주로부터 Tylosin 생산

  • Choi, Du-Bok (Biotechnology Laboratory, B-K Company Ltd.) ;
  • Choi, On-You (United Graduate School of Agricultural Science Shizuoka University) ;
  • Moon, Ok-Ran (Department of Environmental Engineering, Chosun University) ;
  • Yoon, Mi-Ran (Department of Environmental Engineering, Chosun University) ;
  • Ji, Sung-Nam (Department of Environmental Engineering, Chosun University) ;
  • Shin, Dae-Yewn (Department of Environmental Engineering, Chosun University)
  • 최두복 (B-K Company 생명공학 연구실) ;
  • ;
  • 문옥란 (조선대학교 환경공학과) ;
  • 윤미란 (조선대학교 환경공학과) ;
  • 지성남 (조선대학교 환경공학과) ;
  • 신대윤 (조선대학교 환경공학과)
  • Published : 2005.10.31
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Abstract

When rapeseed oil as the carbon source was used for tylosin production from Streptomyces fradiae TP-1239 was very sensitive to oleic acid. Cell growth was restrained by adding 0.8 g/l of oleic acid to the culture broth. Mutant strain TM-224-1 resistant to 1.2 g/l of oleic acid was obtained by screening in solid and liquid media containing oleic acid. The uptake rate of oleic acid by TM-224-1 was approximately 3.8 fold higher than the parent strain. For comparing the TM-224-1 and the parent strain, batch cultures were carried out in a jar fermentor. Cell growth of TM-224-1 strain was higher than the parent strain after two days of culturing. However, after four days of culturing, it was similar to that of the parent strain. The amount of rapeseed oil consumed by TM-224-1 and the parent strain were 60.5 and 78.2 g/l, respectively. The production and yield of tylosin was aproximately 2.0 and 3.2 fold higher than the parent strain, respectively. From these results, it was concluded that this mutant, which was resistant to oleic acid, has improved tylosin production.

Streptomyces fradiae로부터 Tylosin을 효율적으로 생산하기 위해 지방산 내성균주을 분리 했다. 여러 지방산중에서 oleic acid 1.6 g/l 이상이 첨가될 때 세포 성장이 완전히 저해 되었다. 그러나 oleic arid 1.2 g/l에서 얻어진 TM-224-1 균주는 최대 균체농도와 tylosin 생산이 얻어졌다. 또한 oleic acid 소비속도는 parent strain 비해 3.8배 증가했다. Oleic acid 내성균주, TM-224-1 균주와 parent strain을 이용해서 jar fermentor에서 균체농도, tylosin 생산, 그리고 rapeseed oil 소비를 표준조건하에 5일동안 비교하였다. TM-224-1 균주을 이용할 경우 균체 농도는 초기에 parent strain비해 증가했으나 배양중반부터는 감소하기 시작했다. rapeseed oil 소비의 경우는 거의 비슷했다. 그러나 Tylosin 생산 수율은 parent strain 비해 약 3.2배 증가했다.

Keywords

References

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