Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Primary Dermal Irritation Study of Lemon Grass (Cymbopogon citratus) Essential Oil in Rabbits

토끼에서 레몬그라스(Cymbopogon citratus) 정유의 일차피부자극시험

  • Published : 2005.09.01
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Abstract

The present study was carried out to investigate the skin irritation potential of lemon grass essential oil in rabbits. A volume of 0.5 ml of test article was applied to intact and abraded skins, respectively, for 24 h in 6 healthy male New Zealand White rabbits. Parameters measured during 72 h observation period were mortality, clinical signs, body weight changes, and local irritation. All rabbits showed severe edema at both 24 h and 72 h after the application of test article. These animals also showed very slight to severe erythema and/or slight eschar formation at both 24 h and 72 h. The edema and erythema induced by lemon grass essential oil were recovered on day 7 of additional 11-day recovery period, but the eschar formation was not recovered at the end of recovery period. On the other hands, there were no treatment-related adverse effects on clinical sign, body weight and gross finding in rabbits. Based on these results, it was concluded that a single dermal application of the undiluted lemon grass essential oil caused very slight to severe edema, erythema and eschar formation in rabbits and showed a primary irritation index score of 5.63 indicating severe irritation. The results of this study strongly suggest that lemon grass essential oil should be used with care and in highly diluted forms especially when directly applied to the skin.

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References

  1. 김기현,최영신 (2001): 항균 및 항진균작용이 있는 정유 조성물 (대한민국 특허 제 0285781호), 2001년 1월 6일
  2. 김기현 ,최영신 (2002): 유물 및 기록물의 소독을 위하여 허브 정유를 이용한 소독방법 및 소독장치(대한민국 특허 제 0330963호), 2002년 3월 20일
  3. 식품의약품안전청 (1999): 의약품등의 독성시험기준, 식품의약품 안전청 고시1999-61호, 식품의약품안전청, 서울
  4. 신진영, 신동호, 김성호, 배춘식, 박승춘, 김기현, 배주현, 김종춘 (2005): 토끼에서 oregano oil의 일차 피부 자극시험. 대한수의학회지, 45, 39-43
  5. 정해곤,방진기,성낙술,김성민 (2003): 자원식물의 기능성 정유 성분 이용고찰. 한작지 48, 41-48
  6. Bankole, S.A, Joda, A.O. and Ashidi, J.S. (2005): The use of powder and essential oil of Cyrnbopogon citratus against mould deterioration and aflatoxin contamination of 'egusi' melon seeds. J. Basic. Microbiol., 45, 20-30 https://doi.org/10.1002/jobm.200410435
  7. Brophy, J.J., Goldsack, R.J., Fookes, C.J.R and Forster, P.I. (1995): Leaf oils of the genus Backhousia (Myrtaceae). J. Essent. Oil Res., 7, 237-254 https://doi.org/10.1080/10412905.1995.9698514
  8. Choi, D.W., Kim, J.H., Cho, S.Y., Kim, D.H. and Chang, S.Y. (2002): Regulation and quality control of herbal drugs in Korea. Toxicology, 181-182, 581-586 https://doi.org/10.1016/S0300-483X(02)00487-0
  9. Choi, H.S., Song, H.S., Ukeda, H. and Sawamura, M. (2000): Radical-scavenging activities of citrus essential oils and their components: detection using 1, 1-diphenyl-2-picrylhydrazyl. J. Agric. Food Chem., 48, 4156-4161 https://doi.org/10.1021/jf000227d
  10. Craig, A.M., Karchesy, J.J., Blythe, L.L., Gonzalez-Hernandez, M.D.L. and Swan, L.R (2005): Toxicity studies on western juniper oil (Juniperus occidentalis) and Port-Orford-cedar oil (Charnaecyparis lawsoniana) extracts utilizing local lymph node and acute dermal irritation assays. Toxicol. Lett., 154, 217-224 https://doi.org/10.1016/j.toxlet.2004.08.004
  11. de Sousa, A.C., Alviano, D.S., Blank, A.F., Alves, P.B., Alviano, C.S. and Gattass, C.R. (2004): Melissa officinalis L. essential oil: antitumoral and antioxidant activities. J. Pharm. Pharmacol., 56, 677-681 https://doi.org/10.1211/0022357023321
  12. Deng, J.F. (2002): Clinical and laboratory investigations in herbal poisonings. Toxicology, 181-182, 571-576 https://doi.org/10.1016/S0300-483X(02)00484-5
  13. Draize, J.H., Woodard, G. and Calvery, H. (1944): Methods for the study of irritation and toxicity of substances applied topically to the skin and mucous membranes. J. Pharmacol. Exp. Ther., 82, 377-390
  14. Friedman, M., Henika, P.R., Levin, C.E. and Mandrell, R.E. (2004): Antibacterial activities of plant essential oils and their components against Escherichia coli O157 : H7 and Salmonella enterica in apple juice. J. Agric. Food Chem., 52, 6042-6048 https://doi.org/10.1021/jf0495340
  15. Hayes, A.J. and Markovic, B. (2002): Toxicity of Australian essential oil Backhousia citriodora (Lemon myrtle). Part 1. Antimicrobial activity and in vitro cytotoxicity. Food Chem. Toxicol., 40, 535-543 https://doi.org/10.1016/S0278-6915(01)00103-X
  16. Onawunmi, G.O., Yisak, W.A. and Ogunlana, E.O. (1984): Antibacterial constituents in the essential oil of Cymbopagan citratus (DC.) Stapf. J. Ethnopharmacol., 12, 279286
  17. Patrick, E. and Maibach, H.I. (1989): Dermatotoxicology in Principles and Methods of Toxicology (A.W. Hayes, Ed.) Raven Press, New York, pp. 383-406
  18. Prashar, A, Hili, P., Veness, R.G. and Evans, C.S. (2003): Antimicrobial action of palma rosa oil (Cymbopogon martinii) on Saccharomyces cerevisiae. Phytochemistry, 63, 569-575 https://doi.org/10.1016/S0031-9422(03)00226-7
  19. Prashar, A., Locke, I.C. and Evans, C.S. (2004): Cytotoxicity of lavender oil and its major components to human skin cells. Cell Prolif., 37, 221-229 https://doi.org/10.1111/j.1365-2184.2004.00307.x
  20. Sugiura, M., Hayakawa, R., Kato, Y., Sugiura, K. and Hashimoto, R. (2000): Results of patch testing with lavender oil in Japan. Contact Dermatitis, 43, 157-160 https://doi.org/10.1034/j.1600-0536.2000.043003157.x