Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
권호 표지

Phellodendri Cortex Herbal-acupuncture Solution Induced Apoptosis in Human Cervical Cancer Cells, SNU-17

  • Seo, Yong-Seok (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Hanny University) ;
  • Seo, Jung-Chul (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Hanny University) ;
  • Lim, Seong-Chul (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Hanny University) ;
  • Jung, Tae-Young (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Hanny University) ;
  • Han, Sang-Won (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Hanny University)
  • Published : 2005.04.20
Download PDF
⟨ Previous Next ⟩

Abstract

Phellodendri Cortex (PC) has been used traditionally in Korea for damp heat leukorrhea with thick, yellow, discharge, foul-smelling diarrhea or dysentery. We investigated whether the Phellodendri Cortex Herbal-acupuncture Solution (PCHS) induced cell-death on SNU-17, human cervical cancer cell. 3-(4,5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay was performed to find out the cytotoxicity of PCHS. The cell death was identified as apoptosis from the results of 4, 6-diamidineo-2-phenylindole (DAPI) staining, terminal deoxy-nucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay. The expression of proapototic gene, Bax, was increased and the expression of apoptotic gene, Caspase-3, was also increased. Considering the above results, PCHS could induce the apoptosis on SNU-17, human cervical cancer cell, via Bax-related Caspase-3 activation. And it might provide the experimental data for the clinical use of Phellodendri Cortex on cervical cancer.

Keywords